Goat
Expressional analysis of immune-related miRNAs in breast milk
Authors: R.S. Na, G.X. E, W. Sun, X.W. Sun, X.Y. Qiu, L.P. Chen and Y.F. Huang
Immune-related miRNAs in breast milk are extracellular miRNAs that are related to immune organ development and regulation of the immune function in infants and young animals. The goal of this study was to compare the expression levels of five immune-related miRNAs in breast milk in black goats, humans, and dairy cattle. The miRNAs from milk were extracted.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.4
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.4
Copy number and integration sites in growth hormone transgenic goats
Authors: Q. Zhang, J. Lin, Q.H. Yu, W.W. Hu and Q. Yang
Transgenic goats have been utilized for years to produce valuable protein. However, when transgenic goats are produced by random integration of inserted genes into cells, the copy number and integration sites of these genes in the goat genome are typically indefinite. Most polymerase chain reaction (PCR)-based methods that have been utilized to determine .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.10
- DOI:
- 10.4238/2015.March.20.10
Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis
Authors: E.C.B. Silva, M.A. Pelinca, A.C. Acosta, D.M.F. Silva, M.A. Gomes Filho and M.M.P. Guerra
Successful DNA extraction is indispensable for molecular methods based on polymerase chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this study was to evaluate three methods to extract DNA from goat sperm for use in PCR. Eight goat semen pools were used for DNA extraction by using the DNea.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.21
- DOI:
- 10.4238/2014.August.7.21
Genetic diversity and relatedness between Canind?© and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers
Authors: T.S. C?¢mara, J.F. Nunes, F.M. Diniz, G.R. Silva, A.M. Ara?ºjo
The aim of this study was to access the genetic diversity and relatedness between Canind?© and British Alpine goat breeds in the States of Piau? and Cear?¡ using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype .. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019569
- DOI:
- 10.4238/gmr16019569
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
Authors: D?¡rcio ?talo Alves Teixeira, Luciana Magalh?£es Melo, Carlos Alberto de Almeida Gadelha, Rodrigo Maranguape Silva da Cunha, Carlos Bloch Jr., Gandhi R?¡dis-Baptista, Benildo Sousa Cavada and Vicente Jos?© de Figueir?ªdo Freitas
Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In t.. Read More»
- Genet. Mol. Res. 5(1):
Promoter methylation and histone modifications affect the expression of the exogenous DsRed gene in transgenic goats
Authors: M.T. Nuo, J.L. Yuan, W.L. Yang, X.Y. Gao, N. He, H. Liang, M. Cang and D.J. Liu
Transgene silencing, which is common in transgenic plants and animals, limits the generation and application of genetically modified organisms, and is associated with the exogenous gene copy number, the methylation status of its promoters, and histone modification abnormalities. Here, we analyzed the expression of the exogenou.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038560
- DOI:
- 10.4238/gmr.15038560
Stable transfection and identification of a hair follicle-specific expression vector of IGFBP-5 in goat fetal fibroblasts
Authors: X.J. Wang, H.M. Su, Y. Liang, Y.F. Wang, X.D. Guo, Z.G. Wang and D.J. Liu
The insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the 6 members of the IGFBP family and is involved in the regulation of cell growth, apoptosis, and other IGF-stimulated signaling pathways. To determine the significance of IGFBP-5 in the Inner Mongolia Cashmere goat (Capra hircus), a hair follicle-specific expression vector of IGFBP-5, .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.16
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.16
Polymorphisms of the cocaine-amphetamine-regulated transcript (CART) gene and their association with reproductive traits in Chinese goats
Authors: P.Q. Wang, L.M. Deng, B.Y. Zhang, M.X. Chu and J.Z. Hou
Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorp.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1091
- DOI:
- 10.4238/vol10-2gmr1091
Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody
Authors: H.L. Zheng, H. Li, Y.S. Sun, Z.Y. Yang and Q. Yu
Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.25.8
- DOI:
- 10.4238/2014.August.25.8
Genetic relatedness between Ardi, Black Bedouin and Damascus goat breeds
Authors: R.M. Al-Atiyat and R.S. Aljumaah
The present study aimed to analyze genetic relatedness and differentiation of common native goat populations in some countries of the Middle East. The populations were Ardi, Black Bedouin, and Damascus goats in the Kingdom of Saudi Arabia, Jordan, and Syria, respectively. Domesticated goats of the Middle East are mostly relate.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.June.18.8
- DOI:
- 10.4238/2014.June.18.8