Illumina Sequencing
Characterization of the complete Chloroplast genome of Correa carmen, a valuable winter-flowering shrub
Authors: Minghui Chen, Yuxia Zong, Shiping Cheng, Zhilu Zhang
Correa carmen is considered important because of its considerable ornamental and economic value. The most striking characteristic of C. carmen flowers is their long winter-flowering period. In the present study, we generated the first complete C. carmen chloroplast genome sequence based on Illumina paired-end sequencing data. .. Read More»
- Genet. Mol. Res. 16(4):
- gmr16039815
- DOI:
- 10.4238/gmr16039815
Transcriptome profiling of the crofton weed gall fly Procecidochares utilis
Authors: X. Gao, J.Y. Zhu, S. Ma, Z. Zhang, C. Xiao, Q. Li, Z.Y. Li and G.X. Wu
Procecidochares utilis is a tephritid gall fly, which is known to be an effective biological agent that can be used to control the notoriously widespread crofton weed Eupatorium adenophorum. Despite its importance, genetic resources for P. utilis remain scarce. In this study, 1.2 Gb sequences were generated using Illumina pair.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.March.19.1
- DOI:
- 10.4238/2014.March.19.1
Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing
Authors: D.-A. Fang1,2, Y.-F. Zhou2, J.-R. Duan2, M.-Y. Zhang2, D.-P. Xu2, K. Liu2, P. Xu1,2* and Q. Wei3*
RNA-Seq technology has been widely applied to transcriptomics, genomics, molecular marker development, and functional gene studies. In the genome, microsatellites are simple sequence repeats (SSR) with a high degree of polymorphism that are used as DNA markers in many molecular genetic studies. Using traditional methods such as magnetic bead enr.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.1
- DOI:
- 10.4238/2015.November.13.1
Development and characterization of microsatellite markers for Solen grandis using Illumina sequencing approach
Authors: Y.W. Feng, H.L. Jiang, Y.T. Liu, X.P. Wu, X.Q. Liu and X.M. Wei
Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019621
- DOI:
- 10.4238/gmr16019621
Identification of differentially expressed genes associated with flower color in peach using genome-wide transcriptional analysis
Authors: Y. Zhou, X.X. Wu, Z. Zhang and Z.H. Gao
Flower color is an important trait of the ornamental peach (Prunus persica L.). However, the mechanism responsible for the different colors that appear in the same genotype remains unclear. In this study, red samples showed higher anthocyanins content (0.122 ± 0.009 mg/g), which was significantly different from that in white samples (0.066 ±.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.5
- DOI:
- 10.4238/2015.May.11.5
Development and validation of single nucleotide polymorphism markers in Odontobutis potamophila from transcriptomic sequencing
Authors: H.W. Zhang, S.W. Yin, L.J. Zhang, X.Y. Hou, Y.Y. Wang and G.S. Zhang
Transcriptome sequencing technology has been applied in the development and discovery of single nucleotide polymorphism (SNP) markers in fish. In this study, a panel of 120 expressed sequence tag (EST)-derived SNPs was selected by several selection filters from the resultant EST library of Odontobutis potamophila using Illumina Sequencing. In total, 37 SN.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.18
- DOI:
- 10.4238/2015.March.20.18
De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress
Authors: L.Q. Li, J. Li, Y. Chen, Y.F. Lu and L.M. Lu
Tobacco is an economically important crop, and its potassium content can greatly affect the quality of tobacco leaves. However, the molecular mechanism involved in potassium starvation in tobacco has not been elucidated to date. In this study, Illumina (Solexa) sequencing technology was used to analyze the transcriptome of tobacco seedlings under low-pota.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038599
- DOI:
- 10.4238/gmr.15038599
Comparative transcriptome analysis reveals three potential antiviral signaling pathways in lymph organ tissue of the red swamp crayfish, Procambarus clarkii
Authors: Z.-Q. Du
The red swamp crayfish (Procambarus clarkii) is one of the most economically important farmed aquatic species in China. Compared with its relatively well-known antibacterial and antifungal mechanisms, the antiviral mechanism is still unclear. We used Illumina-based RNA sequencing and bioinformatic technology to obtain high-qua.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048858
- DOI:
- 10.4238/gmr15048858
Analysis of alterations to the transcriptome of Loquat (Eriobotrya japonica Lindl.) under low temperature stress via de novo sequencing
Authors: R.G. Gong, J. Lai, W. Yang, M.A. Liao, Z.H. Wang and G.L. Liang
Loquat (Eriobotrya japonica Lindl.), which originates from the cooler hill regions of southwestern China, is a typical subtropical evergreen tree. Loquat is one of the most important economic crops in China, but the available genomic information is very limited. Here, we present the first deep transcriptomic analysis of loquat. De novo assembly generated .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.6
- DOI:
- 10.4238/2015.August.14.6
Identification of genes associated with the increased number of four-seed pods in soybean (Glycine max L.) using transcriptome analysis
Authors: Z.Z. Liu, D. Yao, J. Zhang, Z.L. Li, J. Ma, S.Y. Liu, J. Qu, S.Y. Guan, D.D. Wang, L.D. Pan, D. Wang and P.W. Wang
Seed number per pod is an important component of yield traits in soybean (Glycine max L.). In 2010, we identified a natural mutant with an increased number of four-seed pods from a soybean variety named ‘Jinong 18’ (JN18). Subsequent observations indicated that the trait was stably inherited. To identify and understand the function of genes as.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.39
- DOI:
- 10.4238/2015.December.28.39