Issr
Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays)
Authors: E.C. Oliveira, A.T. Amaral J�?ºnior, L.S.A. Gon�?§alves, G.F. Pena, S.P. Freitas J�?ºnior, R.M. Ribeiro and M.G. Pereira
We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize h.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr767
- DOI:
- 10.4238/vol9-2gmr767
Genetic diversity and population structure of Eucommia ulmoides Oliver, an endangered medicinal plant in China
Authors: J. Yu, Y. Wang, L. Peng, M. Ru and Z.S. Liang
Eucommia ulmoides Oliver, one of the tertiary relict species found only in China, is the only extant species of Eucommiaceae. Using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers, we studied the genetic diversity and population genetic structure of 187 accessions from 17 E. ulmoides populations throughout it.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.30.5
- DOI:
- http://dx.doi.org/10.4238/2015.March.30.5
Genetic diversity of Ceratoides arborescens, a species endemic to China, detected by intersimple sequence repeat (ISSR)
Authors: P.C. Wang, L.L. Zhao, B.T. Mo, Y. Zhang, J. Chen and L.B. Wang
In order to investigate genetic diversity and population structure of Ceratoides arborescens, six populations were selected from different steppe types in Inner Mongolia grasslands of China. Intersimple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among natural populations of C. arborescens. Thirteen ISSR primers gen.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.18
- DOI:
- 10.4238/2015.May.25.18
RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass
Authors: R.S.N de Lima, R.F. Daher, L.S.A. Gon�?§alves, D.A. Rossi, A.T. do Amaral J�?ºnior, M.G. Pereira and F.J.S. L�?©do
Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparativel.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1107
- DOI:
- 10.4238/vol10-3gmr1107
Characterization and identification of ISSR markers associated with oil content in sea buckthorn berries
Authors: J. Ding, C.J. Ruan, Y. Guan, J.Y. Shan, H. Li and Y.H. Bao
Bioactive oils extracted from sea buckthorn (Hippophae rhamnoides) berries contain highly nutritional and medicinal compounds; however, the oil contents of sea buckthorn berries are very low. Thirteen inter-simple sequence repeat (ISSR) primers were used to identify markers associated with oil content of dry pulp in 51 cultivars and lines, which clustered.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038278
- DOI:
- 10.4238/gmr.15038278
Assessment of genetic relationship among Rhododendron cultivars using amplified fragment length polymorphism and inter-simple sequence repeat markers
Authors: J.J. Xu, B. Zhao, H.F. Shen, W.M. Huang and L.X. Yuan
Genetic relationships of 17 Rhododendron cultivars, China, were assessed using inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) markers. A total of 133 bands were obtained using nine selected ISSR primers, 129 (96.99%) of which were polymorphic; 267 bands were amplified by four AFLP primer pairs, 251 (94.01%) of which .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038467
- DOI:
- 10.4238/gmr.15038467
Genetic diversity in natural populations of mangaba in Sergipe, the largest producer State in Brazil
Authors: A.N.R. Soares, M.F. Vit�?³ria, A.L.S. Nascimento, A.S. Ledo, A.R.C. Rabbani and A.V.C. Silva
Mangaba (Hancornia speciosa Gomes) is found in areas of coastal tablelands in the Brazilian Northeast and Cerrado regions. This species has been subjected to habitat fragmentation that is mainly due to human activity, and requires conservation strategies. The aim of this study was to analyze the structure and inter- and intrapopulation genetic diversity o.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038624
- DOI:
- 10.4238/gmr.15038624
Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers
Authors: P.V. Tiago, M.P. Carneiro-Le�?£o, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima
Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará d.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1069
- DOI:
- 10.4238/vol10-3gmr1069
RAPD and ISSR-assisted identification and development of three new SCAR markers specific for the Thinopyrum elongatum E (Poaceae) genome
Authors: G.H. Xu, W.Y. Su, Y.J. Shu, W.W. Cong, L. Wu and C.H. Guo
Diploid Thinopyrum elongatum, a wild relative of wheat, contains many agronomically desirable traits and has potential for increasing genetic variability and introducing desirable characters in this crop. Few molecular markers are available for rapid screening of T. elongatum genome segments in the wheat genetic background. We.. Read More»
- Genet. Mol. Res. 11(2):
- http://dx.doi.org/2012.June.29.7
- DOI:
- http://dx.doi.org/10.4238/2012.June.29.7
Genetic relationships analysis of olive cultivars grown in China
Authors: M.M. Zhan, Z.Z. Cheng, G.C. Su, A.Y. Wang, H.P. Chen, Z.S. Yang, Z. Shan and Q.M. Huang
The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISS.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.13
- DOI:
- 10.4238/2015.June.1.13