Authors: X.B. Qiao, K.Y. Xu, B. Li, X. Luan, T. Xia and X.Z. Fan
We analyzed meat samples of nine pure lines of rabbit and its 37 hybrid combinations by sequencing and single-strand conformation polymorphism techniques to explore genetic polymorphisms of all the three exon regions and part of the 5'-regulatory region of the myostatin (MSTN) gene. Thus, we detected a single nucleotide mutati.. Read More»
Authors: N. Promwatee, B. Laopaiboon, T. Vongpralub, Y. Phasuk,S. Kunhareang, W. Boonkum and M. Duangjinda
Four Thai synthetic chicken lines (Kaen Thong, Khai Mook Esarn, Soi Nin, and Soi Pet) originated from Thai native and exotic commercial chickens were evaluated for their growth and carcass traits with the purpose of developing a Thai broiler breeding program. Insulin-like growth factor I (IGF-I) gene is known to play an important role in growth, prolifera.. Read More»
Authors: J.B. Gao, Y.K. Li, N. Yang, X.H. Ma, C. Adoligbe, B.J. Jiang, C.Z. Fu,G. Cheng and L.S. Zan
The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of bovine Dickkopf homolog 4 (DKK4) are associated with body measurement traits in Qinchuan cattle. By using PCR-SSCP technology and DNA sequencing, we discovered 5 DKK4 SNPs in Qingchuan cattle, including -65G>A and -77G>T in the 5êÂ?Â?-untranslated region, 1532C.. Read More»
Authors: C.H. Dong1, X.M. Song2, L. Zhang2, J.F. Jiang2, J.P. Zhou1 and Y.Q. Jiang2
Prolactin (PRL) plays central roles in mammals’ reproduction, gland development, milk secretion, and the expression of milk protein genes. In dairy cattle, the PRL gene is a potential quantitative trait locus and genetic marker related to milk performance traits. Here, a total of 586 randomly selected Chinese Holstein cows were genotyped for locus P.. Read More»
Authors: Y.F. Zeng, X.Z. Ding, S.R. Cheng and S.J. Yu
Insulin-like growth factor II (IGF-II) plays a key role in mammalian growth and is involved in stimulating fetal cell division, differentiation, and metabolic regulation. IGF-II is considered a candidate gene for genetic markers of growth and carcass traits. Therefore, in this study, the associations of single nucleotide polymorphisms (SNPs) in the IGF-II.. Read More»
Authors: X.H. Du, Q.F. Gan, Z.R. Yuan, X. Gao, L.P. Zhang, H.J. Gao,J.Y. Li and S.Z. Xu1
Myogenic determination factor 1 (MyoD1) and myogenic factor 6 (Myf6) genes belong to the myogenic differentiation (MyoD) gene family, which play key roles in growth and muscle development. The study aimed to investigate the effects of variants in cattle MyoD1 and Myf6 on carcass and meat traits. We screened single nucleotide polymorphisms (SNPs) of both g.. Read More»
Authors: M. Akkurt
Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols .. Read More»
Authors: P. Madesis, I. Ganopoulos, P. Ralli and A. Tsaftaris
The ability to discriminate all species is the ultimate target in barcoding. The Mediterranean basin is a center of origin for legumes and thus they have played a key role in feeding the Mediterranean population. It is also a region with important protected designation of origin and protected geographical indication legumes that provide income in rural ar.. Read More»
Authors: X. Liu, X.Y. Guo, X.Z. Xu, M. Wu, X. Zhang, Q. Li, P.P. Ma, Y. Zhang, C.Y. Wang, F.J. Geng, C.H. Qin, L. Liu, W.H. Shi, Y.C. Wang and Y. Yu
DNA methylation is essential for adipose deposition in mammals. We screened SNPs of the bovine DNA methyltransferase 3b (DNMT3b) gene in Snow Dragon beef, a commercial beef cattle population in China. Nine SNPs were found in the population and three of six novel SNPs were chosen for genotyping and analyzing a possible association with 16 meat quality trai.. Read More»
Authors: Q. Deng, Y. Gao, C.H. Li1,, X.F. Yu, J.S. Ren, S.J. Li, C.Z. Chen, B. Yuan, Y. Ding, H. Jiang and J.B. Zhang
In this study, the performance of 300 Changbaishan Black cattle treated for superovulation from June to September was evaluated to determine the optimal conditions and herds for bovine embryo production. Data analysis revealed that cattle treated in July and August had higher numbers of available embryos (NAE), M1 embryos (NM1.. Read More»