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Animal Genetics   Research Article

Rabbit MSTN gene polymorphisms and genetic effect analysis

Authors: X.B. Qiao, K.Y. Xu, B. Li, X. Luan, T. Xia and X.Z. Fan

We analyzed meat samples of nine pure lines of rabbit and its 37 hybrid combinations by sequencing and single-strand conformation polymorphism techniques to explore genetic polymorphisms of all the three exon regions and part of the 5'-regulatory region of the myostatin (MSTN) gene. Thus, we detected a single nucleotide mutati.. Read More»

Genet. Mol. Res. 13(2):
2014.April.8.1
DOI:
10.4238/2014.April.8.1
Animal Genetics   Research Article

Insulin-like growth factor I gene polymorphism associated with growth and carcass traits in Thai synthetic chickens

Authors: N. Promwatee, B. Laopaiboon, T. Vongpralub, Y. Phasuk,S. Kunhareang, W. Boonkum and M. Duangjinda

Four Thai synthetic chicken lines (Kaen Thong, Khai Mook Esarn, Soi Nin, and Soi Pet) originated from Thai native and exotic commercial chickens were evaluated for their growth and carcass traits with the purpose of developing a Thai broiler breeding program. Insulin-like growth factor I (IGF-I) gene is known to play an important role in growth, prolifera.. Read More»

Genet. Mol. Res. 12(4):
2013.March.15.6
DOI:
10.4238/2013.March.15.6
Animal Genetics   Research Article

Novel SNPs in the exon region of bovine DKK4 gene and their association with body measurement traits in Qinchuan cattle

Authors: J.B. Gao, Y.K. Li, N. Yang, X.H. Ma, C. Adoligbe, B.J. Jiang, C.Z. Fu,G. Cheng and L.S. Zan

The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of bovine Dickkopf homolog 4 (DKK4) are associated with body measurement traits in Qinchuan cattle. By using PCR-SSCP technology and DNA sequencing, we discovered 5 DKK4 SNPs in Qingchuan cattle, including -65G>A and -77G>T in the 5êÂ?Â?-untranslated region, 1532C.. Read More»

Genet. Mol. Res. 12(4):
2013.February.28.30
DOI:
10.4238/2013.February.28.30
Animal Genetics   Research Article

New insights into the prolactin-RsaI (PRL-RsaI) locus in Chinese Holstein cows and its effect on milk performance traits

Authors: C.H. Dong1, X.M. Song2, L. Zhang2, J.F. Jiang2, J.P. Zhou1 and Y.Q. Jiang2

Prolactin (PRL) plays central roles in mammals’ reproduction, gland development, milk secretion, and the expression of milk protein genes. In dairy cattle, the PRL gene is a potential quantitative trait locus and genetic marker related to milk performance traits. Here, a total of 586 randomly selected Chinese Holstein cows were genotyped for locus P.. Read More»

Genet. Mol. Res. 12(4):
2013.November.22.3
DOI:
10.4238/2013.November.22.3
Human Genetics   Research Article

Genetic polymorphisms of the IGF-II gene intron 8 coding region and its association with growth and carcass traits in yak

Authors: Y.F. Zeng, X.Z. Ding, S.R. Cheng and S.J. Yu

Insulin-like growth factor II (IGF-II) plays a key role in mammalian growth and is involved in stimulating fetal cell division, differentiation, and metabolic regulation. IGF-II is considered a candidate gene for genetic markers of growth and carcass traits. Therefore, in this study, the associations of single nucleotide polymorphisms (SNPs) in the IGF-II.. Read More»

Genet. Mol. Res. 12(4):
2013.December.11.11
DOI:
10.4238/2013.December.11.11
Cytogenomics   Research Article

Polymorphism of MyoD1 and Myf6 genes and associations with carcass and meat quality traits in beef cattle

Authors: X.H. Du, Q.F. Gan, Z.R. Yuan, X. Gao, L.P. Zhang, H.J. Gao,J.Y. Li and S.Z. Xu1

Myogenic determination factor 1 (MyoD1) and myogenic factor 6 (Myf6) genes belong to the myogenic differentiation (MyoD) gene family, which play key roles in growth and muscle development. The study aimed to investigate the effects of variants in cattle MyoD1 and Myf6 on carcass and meat traits. We screened single nucleotide polymorphisms (SNPs) of both g.. Read More»

Genet. Mol. Res. 12(4):
dx.doi.org/2013.December.13.4
DOI:
dx.doi.org/10.4238/2013.December.13.4
Microbial Genetics   Research Article

Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.)

Authors: M. Akkurt

Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols .. Read More»

Genet. Mol. Res. 11(3):
2012.August.13.8
DOI:
10.4238/2012.August.13.8
Plant Genetics   Research Article

Barcoding the major Mediterranean leguminous crops by combining universal chloroplast and nuclear DNA sequence targets

Authors: P. Madesis, I. Ganopoulos, P. Ralli and A. Tsaftaris

The ability to discriminate all species is the ultimate target in barcoding. The Mediterranean basin is a center of origin for legumes and thus they have played a key role in feeding the Mediterranean population. It is also a region with important protected designation of origin and protected geographical indication legumes that provide income in rural ar.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.10
DOI:
10.4238/2012.July.10.10
Animal Genetics   Research Article

Novel single nucleotide polymorphisms of the bovine methyltransferase 3b gene and their association with meat quality traits in beef cattle

Authors: X. Liu, X.Y. Guo, X.Z. Xu, M. Wu, X. Zhang, Q. Li, P.P. Ma, Y. Zhang, C.Y. Wang, F.J. Geng, C.H. Qin, L. Liu, W.H. Shi, Y.C. Wang and Y. Yu

DNA methylation is essential for adipose deposition in mammals. We screened SNPs of the bovine DNA methyltransferase 3b (DNMT3b) gene in Snow Dragon beef, a commercial beef cattle population in China. Nine SNPs were found in the population and three of six novel SNPs were chosen for genotyping and analyzing a possible association with 16 meat quality trai.. Read More»

Genet. Mol. Res. 11(3):
2012.June.29.1
DOI:
10.4238/2012.June.29.1
Plant Genetics   Research Article

Effects of choice of month of treatment and parity order on bovine superovulation traits

Authors: Q. Deng, Y. Gao, C.H. Li1,, X.F. Yu, J.S. Ren, S.J. Li, C.Z. Chen, B. Yuan, Y. Ding, H. Jiang and J.B. Zhang

In this study, the performance of 300 Changbaishan Black cattle treated for superovulation from June to September was evaluated to determine the optimal conditions and herds for bovine embryo production. Data analysis revealed that cattle treated in July and August had higher numbers of available embryos (NAE), M1 embryos (NM1.. Read More»

Genet. Mol. Res. 14(4):
2015.November.24.14
DOI:
10.4238/2015.November.24.14