Microsatellite Loci
Isolation and characterization of polymorphic microsatellite loci from aerial yam (Dioscorea bulbifera L.)
Authors: Q.Q. Yan, Y. Li, X.Q. Sun, J.L. Guo, Y.Y. Hang and M.M. Li
Dioscorea bulbifera L. is widely distributed in pantropical regions along the equator. The taxonomic treatment of this species is ambiguous due to its extreme polymorphic morphological characters. In order to provide tools to facilitate the study of genetic diversity, population structure, patterns of gene flow, and the mating.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.12.3
- DOI:
- http://dx.doi.org/10.4238/2014.March.12.3
Development and characterization of microsatellite loci in Excentrodendron hsienmu (Chun & How) H.T. Chang & R.H. Miau
Authors: W.H. Shen, Z.H. Li, Y.H. Peng, M.H. Yang, Z.Q. Tan and W. Zheng
Microsatellite markers were isolated using dual-suppression-PCR for the endangered species Excentrodendron hsienmu (Tiliaceae) to evaluate the genetic diversity and population structure of this species. A total of 11 polymorphic microsatellite loci were characterized in E. hsienmu. The number of alleles per locus ranged from 2 to 9, with an average of 5.2.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038060
- DOI:
- 10.4238/gmr.15038060
Isolation and characterization of microsatellite loci from an endangered tree species, Toona ciliata var. pubescens
Authors: J. Liu Z.-X. Sun Y.-T. Chen J.-M. Jiang
Toona ciliata var. pubescens is considered an endangered tree species native to China. In order to help develop a conservation program for this species, we evaluated its genetic diversity and population genetics. We isolated microsatellite DNA loci using streptavidin beads. A genomic library, enriched with microsatellites, was constructed and screened by .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.19.4
- DOI:
- 10.4238/2012.September.19.4
Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae)
Authors: J.-T. Tian Z.-H. Liu L.-Q. Zhou B. Wu P. Liu A.-G. Yang
Blood clams (Scapharca broughtonii) are widely cultivated and consumed in noutheast Asia. Forty-eight polymorphic microsatellite loci were developed for this clam using magnetic-bead hybridization enrichment. The number of alleles per locus ranged from 2 to 14. Polymorphism of these loci was assessed in 30 individuals from a population collected from coas.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.11.2
- DOI:
- 10.4238/2012.October.11.2
Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae)
Authors: H. Li, Z.-S. Zhou, J. Guo and L.H. Lin
We isolated and characterizated 12 polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae). The loci were screened in 37 E. velox individuals. The number of alleles ranged from 6 to 16. The observed heterozygosity ranged from 0.432 to 0.919, and the expected heterozygosity ranged from 0.685 to 0.902. These microsatelli.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.9.5
- DOI:
- 10.4238/2012.October.9.5
Isolation and characterization of microsatellite markers for the Korean rockfish, Sebastes schlegeli
Authors: C.-C. Bai, S.-F. Liu, Z.-M. Zhuang, L. Lin, H. Wang and Y.-Q. Chang
The Korean rockfish (Sebastes schlegeli) is an important commercial fish that is widely used in aquaculture. We isolated and characterized 18 polymorphic microsatellite loci from the Korean rockfish using a (GT)13-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1522
- DOI:
- 10.4238/vol10-3gmr1522
Development of polymorphic microsatellites for Sillago sihama based on next-generation sequencing and transferability to Sillago japonica
Authors: R.X. Wu, H.R. Zhang, S.F. Niu, Y. Zhai and X.F. Liu
Sillago sihama (Forsskål, 1775), a commercially important marine fishery species in the Indo-West Pacific, is being developed as a target species for aquaculture and stock enhancement in China. However, due to the limitations of traditional isolation methods, the available microsatellite loci, or simple sequence repeats .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049046
- DOI:
- 10.4238/gmr15049046
Polymorphic microsatellite loci for the Japanese anchovy Engraulis japonicus (Engraulidae)
Authors: L. Lin, L. Zhu, S.-F. Liu, Y.-Q. Su and Z.-M. Zhuang
We isolated and characterized 14 polymorphic microsatellite loci in the Japanese anchovy (Engraulis japonicus) using a (GT)13-enriched genomic library. The numbers of alleles per locus ranged from 6 to 31, with a mean of 17.8. The observed and expected heterozygosities ranged from 0.180 to 0.949 and from 0.172 to 0.966, with means of 0.731 and 0.825, resp.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1085
- DOI:
- 10.4238/vol10-2gmr1085
Genetic characterization of hatchery populations of Korean spotted sea bass (Lateolabrax maculatus) using multiplex polymerase chain reaction assays
Authors: H.S. An, H.Y. Kim, J.B. Kim, D.S. Chang, K.D. Park, J.W. Lee, J.I. Myeong and C.M. An
The spotted sea bass, Lateolabrax maculatus, is an important commercial and recreational fishery resource in Korea. Aquacultural production of this species has increased because of recent resource declines, growing consumption, and ongoing government-operated stock release programs. Therefore, the genetic characterization of h.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.14
- DOI:
- 10.4238/2014.August.28.14
Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota
Authors: J.B. Shangguan, Z.B. Li, Y.F. Ning, Y.S. Huang, Y. Yuan, J. Lu, B.B. Li and X.Q. Mao
The sea cucumber Holothuria leucospilota has high medicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic m.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.12.9
- DOI:
- 10.4238/2015.June.12.9