Authors: H. Xue, C.H. Lu and X.B. Wu
We isolated and characterized 11 microsatellite loci for the Chinese yew, Taxus chinensis var. mairei, an endangered tree species in China, by constructing a (CA)12-enriched library. The number of alleles per locus ranged from 5 to 10. The observed heterozygosities ranged from 0.2500 to 0.8333 and the expected heterozygosities.. Read More»
Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang
The small yellow croaker (Larimichthys polyactis) is a highly valued fish for human consumption found in the Western Pacific that was considered endangered until recently because of overfishing. We selected microsatellite markers for this species from markers developed for Miichthys miiuy, also of the family Sciaenidae. Among .. Read More»
Authors: C.-C. Bai, S.-F. Liu, Z.-M. Zhuang, Y.-J. Yuan, H.-B. Liu and F.-Q. Dai
Synechogobius hasta is an important commercial marine fish with distinctive features of rapid growth and short lifespan. We isolated and characterized 17 microsatellite markers for S. hasta using a (GT)13-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern .. Read More»
Authors: Q.H. Gu, B.X. Xiong, Y.T. Zhu, Q. Wang and P.S. Shi
Although it is a major freshwater gastropod species, genetic diversity of Bellamya aeruginosa was completely unknown. Eighteen microsatellite loci were isolated and characterized from (AC)15-enriched genomic libraries of the freshwater snail B. aeruginosa. Most of the 18 loci were successfully amplified and high polymorphic information content values were.. Read More»
Authors: Y.W. Feng, H.L. Jiang, Y.T. Liu, X.P. Wu, X.Q. Liu and X.M. Wei
Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp.. Read More»
Authors: Isolation and characterization of 8 microsatellite loci from Chrysophyllum gonocarpum (Sapotaceae)
Chrysophyllum gonocarpum is a tropical tree species that is very important in the recovery of heterogeneous forests and of degraded areas of permanent preservation. We identified microsatellite loci for C. gonocarpum to assess the genetic variability and the patterns of the population structure of the species. We isolated 8 microsatellite primers by using CT.. Read More»
Authors: E.S.L. Santos
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Authors: Y.F. Ning, Z.B. Li, Q.H. Li, G. Dai, J.B. Shangguan, Y. Yuan and Y.S. Huang
The rabbitfish Siganus fuscescens is an economically valuable species that is widely distributed throughout the estuaries, intertidal, and offshore coasts of the Indo-Pacific and eastern Mediterranean. Ten novel microsatellite loci from the genome of S. fuscescens were developed using the fast isolation protocol with amplified fragment length polymorphism.. Read More»
Authors: M.F. Alves P.A.V. Barroso A.Y. Ciampi L.V. Hoffmann V.C.R. Azevedo U. Cavalcante
Gossypium mustelinum is the only cotton species native to Brazil; it is endemic to the semi-arid region of the northeast. The populations are found near perennial and semi-perennial sources of water, such as ponds or pools in intermittent streams. Problems with in situ conservation derive from human interference in its habitat, mainly because of excessive.. Read More»
Authors: H.-T. Ma, H.-B. Jiang, X.-Q. Liu, X.-P. Wu and X.-M Wei
The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individua.. Read More»