Microsatellite
Isolation and characterization of novel polymorphic microsatellite loci in Perinereis aibuhitensis
Authors: Y. Liu, D. Yu, Q. Wang, H. Liu, S. Guan and M. Liu
Perinereis aibuhitensis is a commercially and ecologically important intertidal worm. In this study, eight polymorphic microsatellite loci were isolated in this species for the first time, and a wild population was used to estimate the properties of these loci. The number of alleles per locus ranged from 6 to 20, and the observed and expected heterozygosi.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017144
- DOI:
- 10.4238/gmr.15017144
Molecular characterization of patchouli (Pogostemon spp) germplasm
Authors: S.S. Sandes1, M.I. Zucchi2, J.B. Pinheiro3, M.M. Bajay3, C.E.A. Batista3,F.A. Brito1, M.F. Arrigoni-Blank1, S.V. Alvares-Carvalho1, R. Silva-Mann1 and A.F. Blank1
atchouli [Pogostemon cablin (Blanco) Benth.] is an aromatic, herbaceous plant belonging to the Lamiaceae family native to Southeast Asia. Its leaves produce an essential oil regularly used by the perfume and cosmetics industries. However, since patchouli from the Philippines and India were described and named Pogostemon patchouli, there has been a diverge.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017458
- DOI:
- 10.4238/gmr.15017458
Development of microsatellite markers in the tetraploid fern Ceratopteris thalictroides (Parkeriaceae) using RAD tag sequencing
Authors: X.Y. Yang1*, Z.C. Long2*, A.W. Gichira2, Y.H. Guo1, Q.F. Wang2 and J.M. Chen2
To understand the genetic variability of the tetraploid fern Ceratopteris thalictroides (Parkeriaceae), we described 30 polymorphic microsatellite markers obtained using the restriction site-associated DNA (RAD) tag sequencing technique. A total of 26 individuals were genotyped for each marker. The number of alleles per locus ranged from 4 to 10, and the .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017550
- DOI:
- 10.4238/gmr.15017550
Molecular diversity of Pakistani mango (Mangifera indica L.) varieties based on microsatellite markers
Authors: T. Nazish, G. Shabbir, A. Ali, S. Sami-ul-Allah, M. Naeem, M. Javed,S. Batool, H. Arshad, S.B. Hussain, K. Aslam, R. Seher, M. Tahir and M. Baber
Understanding the genetic diversity of different Pakistani mango varieties is important for germplasm management and varietal characterization. Microsatellites are efficient and highly polymorphic markers for comparative genome mapping, and were used in the present study to determine the genetic relatedness and variability amo.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029560
- DOI:
- 10.4238/gmr16029560
New set of microsatellites for Chinese tallow tree, Triadica sebifera
Authors: Y.F. Zhuang, Z.F. Wang and L.F. Wu
Chinese tallow (Triadica sebifera) is an important crop and ornamental tree. After it was introduced into the USA, it gradually became a noxious invasive tree in south-eastern America since the middle of the 1900s. Because only six microsatellites were reported previously in T. sebifera, to better understand the genetic divers.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029624
- DOI:
- 10.4238/gmr16029624
Development of a polymorphic short tandem repeat locus multiplex system for efficient human identification
Authors: R.G. Rodovalho, E.L. Rodrigues, G.S. Santos, L.M. Cavalcanti,P.R. Lima, A.G. Rodovalho, R.G. Vital, M.A.D. Gigonzac and A.D. da Cruz
This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S44.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029557
- DOI:
- 10.4238/gmr16029557
Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples
Authors: M.I. Espinosa, A. Bertin, F.A. Squeo, A. Cort�©s and N. Gouin
Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control regi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.13
- DOI:
- 10.4238/2015.January.23.13
Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm
Authors: C.P. Santos, J.B. Pinheiro, M.I. Zucchi, M.M. Bajay, J.B. Campos, M.F. Arrigoni-Blank, J.A.O. Pinto and A.F. Blank
The conservation of plants in germplasm banks ensures the characterization and availability of these resources for future generations. The present study used DNA markers to obtain genetic information about germplasm collections of Lippia sidoides and L. gracilis, which are maintained in an Active Germplasm Bank (AGB). Genetic variability of samples in the.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037781
- DOI:
- 10.4238/gmr.15037781
Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)
Authors: Z.B. Li, Y.S. Huang, J.B. Shangguan, Y.F. Ning, Y. Yi and G. Dai
Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.August.28.5
- DOI:
- http://dx.doi.org/10.4238/2015.August.28.5
Isolation and characterization of microsatellite loci for Cunninghamia lanceolata (Lamb.) Hook
Authors: Y.X. Li, Z.S. Wang, J.K. Sui, Y.F. Zeng, A.G. Duan and J.G. Zhang
As a result of human activities, wild populations of Cunninghamia lanceolata (Cupressaceae) have sharply declined in recent years. The development and implementation of a valid conservation strategy require a clear understanding of the genetic makeup of this species. Eleven polymorphic microsatellite loci were isolated and characterized from samples of 52.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.19
- DOI:
- 10.4238/2015.January.23.19