Microsatellite
Isolation and characterization of polymorphic microsatellite loci in Aleurodicus dispersus (Hemiptera, Aleyrodidae)
Authors: G.-C. Ma, X. Wu, C.-L. Ma, H.-L. Wu, H.-Y. Hu, L.-M. Niu and Y.-G. Fu
Ten microsatellite markers were isolated and characterized from Aleurodicus dispersus, the spiraling whitefly, an exotic pest species that is considered to be one of the most serious agricultural pests on Hainan Island, China. The polymorphism of these loci was examined in individual whiteflies from Hainan Island and from the .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.18.2
- DOI:
- 10.4238/2011.October.18.2
Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies
Authors: A.C. Kelly, N.E. Mateus-Pinilla, M. Douglas, M. Douglas, P. Shelton and J. Novakofski
Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for pop.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.19.1
- DOI:
- 10.4238/2011.October.19.1
Isolation and characterization of microsatellite loci in Sisyrinchium (Iridaceae) and cross amplification in other genera
Authors: R.B. Miz, L.O. Tacuati�¡, F.W. Cidade, A.P. de Souza, F. Bered, L. Eggers and T.T. de Souza-Chies
Recent phylogenetic studies on Sisyrinchium strongly suggest that species classified in section Hydastylus and section Viperella belong to a single group of plants in recent adaptive radiation (Clade IV). These species neither present clear morphological differentiation among them nor show clear identification using DNA barcode markers. Thus, the main goa.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038474
- DOI:
- 10.4238/gmr.15038474
A set of novel microsatellite markers developed for an economically important tree, Dracontomelon duperreanum, in China
Authors: Z.F. Wang, H.L. Cao, L.F. Wu, Y. Guo, Q.M. Mei, M. Li, Y. Wang and Z.M. Wang
Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associ.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029578
- DOI:
- 10.4238/gmr16029578
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila
Authors: P.P. Wang, M. Ren, S.Q. Chen, S.W. Yin, C. Zhao, H.Y. Zhang, X.R. Li, Q.Q. Cao and G.Q. Zhou
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequen.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029129
- DOI:
- 10.4238/gmr16029129
Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae
Authors: B.B. Cheng, Q.W. Sun,Y.Q. Zheng
Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.7.15
- DOI:
- 10.4238/2015.December.7.15
New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis
Authors: C.X. Tian, X.-F. Liang, M. Yang, Y.Q. Dou, H.Z. Zheng, L. Cao,Y.C. Yuan and C. Zhao
The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1... Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.22.1
- DOI:
- 10.4238/2014.January.22.1
Application of functional genomic information to develop efficient EST-SSRs for the chicken (Gallus gallus)
Authors: M.R. Bakhtiarizadeh, B. Arefnejad, E. Ebrahimie and M. Ebrahimi
Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity.. Read More»
- Genet. Mol. Res. 11(2):
- dx.doi.org/2012.May.21.12
- DOI:
- dx.doi.org/10.4238/2012.May.21.12
Isolation and characterization of novel microsatellite loci in the wrinkled frog
Authors: D.Y. Kim1*, D. Kim2*, S.K. Park2, H. Lee1, H.Y. Suk2 and M.S. Min1
The wrinkled frog, Rana rugosa, is a species complex that inhabits plains and mountains near freshwater bodies throughout East Asia, encompassing China, Korea, Japan, and the Russian Primorye region. Although extensive efforts are required to estimate cryptic diversity in the R. rugosa complex, no specifically designed microsatellite loci are available. H.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017183
- DOI:
- 10.4238/gmr.15017183
Allele frequencies of microsatellite loci for genetic characterization of a Sicilian bovine population
Authors: M. Cosenza, S. Reale, T. Lupo, F. Vitale and S. Caracappa
Short tandem repeats are used as an effective method to trace DNA markers in genotyping. Using a standardized kit, we tested 11 microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in a sample of 495 Sicilian cattle. The aim of this study was to investigate the allele frequencies in the Sicilian cattle population to prov.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.12
- DOI:
- 10.4238/2015.January.30.12