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Microsatellite

Human Genetics   Research Article

Microsatellite marker analysis reveals the distinction between the north and south groups of hard clam (Meretrix meretrix) in China

Authors: X.F. Gu, Y.H. Dong, H.H. Yao, X.L. Zhou, X.Y. Qi and Z.H. Lin

Meretrix meretrix is one of the important commercial bivalves in China. A total of 198 individual clams were collected from 5 locations characteristic of the clam’s 5 main natural habitats in China, that is, Shandong, Jiangsu, Fujian, Guangdong, and Guangxi. Ten polymorphic microsatellite markers were selected to examine the genetic diversity and id.. Read More»

Genet. Mol. Res. 14(1):
2015.February.6.23
DOI:
10.4238/2015.February.6.23
Plant Genetics   Research Article

Next-generation sequencing identification and characterization of microsatellite markers in Aconitum austrokoreense Koidz., an endemic and endangered medicinal plant of Korea

Authors: Y.-E. Yun, J.-N. Yu, G.H. Nam, S.-A. Ryu, S. Kim, K. Oh and C.E. Lim

We used next-generation sequencing to develop 9 novel microsatellite markers in Aconitum austrokoreense, an endemic and endangered medicinal plant in Korea. Owing to its very limited distribution, over-harvesting for traditional medicinal purposes, and habitat loss, the natural populations are dramatically declining in Korea. All novel microsatellite mark.. Read More»

Genet. Mol. Res. 14(2):
2015.May.11.13
DOI:
10.4238/2015.May.11.13
Plant Genetics   Research Article

Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)

Authors: Z.Z. Tian F.Q. Zhang, Z.Y. Cai and S.L. Chen

Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequ.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038406
DOI:
10.4238/gmr.15038406
Plant Genetics   Research Article

Development of expressed sequence tag-based microsatellite markers for the critically endangered Iso�«tes sinensis (Isoetaceae) based on transcriptome analysis

Authors: A.W. Gichira, Z.C. Long, Q.F. Wang, J.M. Chen and K. Liao

Isoëtes sinensis is a critically endangered quillwort. To facilitate studies on the conservation genetics of this species, we developed expressed sequence tag-simple sequence repeat (EST-SSR) markers. A total of 50,063 unigenes were predicted by transcriptome sequencing, 5294 (10.6%) of which significantly matched 3011 Gene Ontology annotations and 2.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038497
DOI:
10.4238/gmr.15038497
Cytogenomics   Research Article

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species

Authors: G.M. Guimar�£es-Marques J.S. Batista H.M. Guimar�£es M.V. Naice-Daou M.P. Lima K.M. Formiga J.M.M. Santos C.A.P. Lima M.S. Rafael

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity rang.. Read More»

Genet. Mol. Res. 11(4):
2012.December.6.3
DOI:
10.4238/2012.December.6.3
Animal Genetics   Research Article

Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona

Authors: M.V.C. Viana, E.A. Miranda, A.K. de Francisco, C.A.L. Carvalho and A.M. Waldschmidt

Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intrac.. Read More»

Genet. Mol. Res. 10(4):
2011.November.22.11
DOI:
10.4238/2011.November.22.11
Plant Genetics   Research Article

Genetic divergence of common bean cultivars

Authors: J.S. Veloso, W. Silva, L.R. Pinheiro, J.B. dos Santos, N.S. Fonseca Jr. and M.P. Euzebio

The aim of this study was to evaluate genetic divergence in the ‘Carioca’ (beige with brown stripes) common bean cultivar used by different institutions and in 16 other common bean cultivars used in the Rede Cooperativa de Pesquisa de Feijão (Cooperative Network of Common Bean Research), by using simple sequence repeats associated with .. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.22.22
DOI:
http://dx.doi.org/10.4238/2015.September.22.22
Animal Genetics   Research Article

Development of novel microsatellite markers in the Korean rockfish Sebastes schlegeli

Authors: H.-T. Ma, C.-F. Jia, J.-M. Yang, F. Wang, R. Xue, C.-H. Han and H.-B. Jiang

The Korean rockfish Sebastes schlegeli is a valuable recreational and commercial fish in China, and is cultured in land-based tanks and net cages. Fifteen microsatellite markers were developed for this species, and their polymorphisms were examined in a population. The allele number of the 15 markers ranged from 2 to 13, with an average of 5.933 per locus.. Read More»

Genet. Mol. Res. 14(2):
2015.May.12.12
DOI:
10.4238/2015.May.12.12
Microbial Genetics   Short communication

Microsatellite markers for Amazon pellona Pellona castelnaeana (Clupeiformes: Pristigasteridae)

Authors: A.M. Ximenes, E.J. Hern�¡ndez-Ruz, V.N. Machado, L.R.R. Rodrigues, G.C.S. Ritter and I.P. Farias

The Amazon pellona is one of the few species of Pristigasteridae with recognized commercial value in the Amazon. We isolated 24 and characterized 8 microsatellite loci for this species. The number of alleles ranges from 2-8 per locus. Observed heterozygosities ranged from 0.052-0.823, while expected heterozygosities from 0.052-0.836. These 8 microsatellit.. Read More»

Genet. Mol. Res. 14(2):
2015.May.18.1
DOI:
10.4238/2015.May.18.1
Animal Genetics   Research Article

Genetic structure and diversity in natural and stocked populations of the mandarin fish (Siniperca chuatsi) in China

Authors: M. Yang, C. Tian, X.-F. Liang, H. Zheng, C. Zhao and K. Zhu

The Chinese perch, or mandarin fish (Siniperca chuatsi), is a freshwater fish that is endemic to East Asia. In this study, we investigated the genetic diversity and structure of nine natural mandarin fish populations (from the Yangtze River and Amur River basins) and six hatchery stocks (from central and south China) using microsatellite markers. The resu.. Read More»

Genet. Mol. Res. 14(2):
2015.May.18.5
DOI:
10.4238/2015.May.18.5