Authors: L. Liu, L. Lin, C.H. Li, S.N. Xu, Y. Liu and Y.B. Zhou
We isolated and characterized 22 polymorphic microsatellite loci in Lutjanus erythropterus using a (GT)13-enriched genomic library. We found between 2 and 8 alleles per locus, with a mean of 4.85. The observed and expected heterozygosities ranged from 0.065 to 0.867 and from 0.085 to 0.832, respectively, with means of.. Read More»
Authors: X.Q. Mao, Z.B. Li, Y.F. Ning, J.B. Shangguan, Y. Yuan Y.S. Huang and B.B. Li
Rabbitfish, Siganus fuscescens, is widely distributed in the Indo-Pacific regions and eastern Mediterranean. Its dwelling place includes reef flats, coral reef regions, and seagrass meadows in tropical area and reef areas or shallow waters in locations at high latitudes. In the present study, 10 new polymorphic microsatellite markers were screened from 30.. Read More»
Authors: S. Arlud, G.X. E and S.C. Zeng
Microsatellite DNA markers, which are assumed to drift, have been widely used to assess genetic diversity in all major domestic animal species. These markers provide insight into the arrival and dispersion history of a species, with regard to their content or management history. However, no direct evidence supports current standard microsatellite markers .. Read More»
Authors: T. Li, J. Zhao, W. Li, Y. Shi, X.Y. Hong and X.P. Zhu,
Pelodiscus sinensis is a common freshwater soft-shell turtle found in China, and is an important aquaculture species. In this study, 20 polymorphic microsatellite primers were developed from the transcriptome. The genetic diversity of three populations of P. sinensis was evaluated, using 72 individuals. The number of alleles per locus ranged from 3 to 26... Read More»
Authors: S. Peyachoknagul, C. Nettuwakul, P. Phuekvilai, S. Wannapinpong and K. Srikulnath
Vandaceous orchids are a group of orchid genera in the subfamily Vandoideae. Among this group, Mokara, Phalaenopsis, and Vanda are the most popular and commercially important orchids in Thailand. Novel microsatellite markers were developed from Mokara, the intergeneric hybrid from 3 genera Vanda.. Read More»
Authors: M.A. Moraes, T.Y.K. Kubota, E.C.B. Silva, A.M. Silva, J. Cambuim, M.L.T. Moraes, E. Furlani Junior and A.M. Sebbenn
Hymenaea stigonocarpa is a deciduous and monoecious Neotropical tree species pollinated by bats. Due to overexploitation and habitat destruction, the population size has drastically diminished in nature. No previous study has investigated Mendelian inheritance, linkage, and genotypic disequilibrium in the available microsatellite markers in this species. .. Read More»
Authors: P.R.O. Silva, O.N.J. Jesus, S. Creste, A. Figueira, E.P. Amorim and C.F. Ferreira
Microsatellite markers have been widely used in the quantification of genetic variability and for genetic breeding in Musa spp. The objective of the present study was to evaluate the discriminatory power of microsatellite markers derived from ‘Calcutta 4’ and ‘Ouro’ genomic libraries, and to analyze the genetic variability among 30.. Read More»
Authors: M. Ruiz-GarcÃ?Âa, M. Pinedo-Castro, K. Luengas-Villamil, C. VergaraJ.A. Rodriguez and J.M. Shostell
We sequenced the mitochondrial DNA (mtDNA) control region of 59 peccaries (44 white-lipped peccaries, Tayassu pecari, and 15 collared peccaries, Pecari tajacu). We also genotyped 3 DNA microsatellites from 78 white-lipped peccaries representing the 4 putative morphological subspecies (i.e., spiradens, aequatoris, pecari, and albirostris) present in northw.. Read More»
Authors: G.C. Yu, Q.Z. Tang, K.R. Long, T.D. Che, M.Z. Li and S.R. Shuai
Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleoti.. Read More»
Authors: W.C. Yang, K.Q. Tang, J. Mei, W.B. Zeng and L.G. Yang
Chinese native buffaloes have faced the threat of extinction, along with an increase in crossbreeding with domesticated river buffaloes; consequently, conservation of local buffalo genetic resources has become a priority. A Chinese native breed, Jianghan, is often crossed intentionally and unintentionally with imported breeds .. Read More»