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Microsatellite

Animal Genetics   Subspeciality Notes

Isolation and characterization of polymorphic microsatellite loci in the hard-shelled mussel, Mytilus coruscus (Mytilidae)

Authors: T.-J. Xu, Y.-N. Sun, Y.-T. Yuan, Z. Liao and R.-X. Wang

Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to sev.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr856
DOI:
10.4238/vol9-3gmr856
Human Genetics   Research Article

High frequency of microsatellite instability in sporadic colorectal cancer patients in Iran

Authors: M. Moghbeli, O. Moaven, E. Dadkhah, M. Farzadnia, N.M. Roshan, H. Asadzadeh-Aghdaee, M.M. Bahar, R. Raeisossadati, M.M. Forghanifard, S.R.E.A. Bakhtiari, A. Baradaran and M.R. Abbaszadegan

Microsatellite instability in sporadic colorectal cancer patients was assessed, and the clinicopathological associations were evaluated in northeastern Iran, which is a high-risk region for gastrointestinal malignancies. Microsatellite instability (MSI)status of tumoral tissue, compared to normal tissue, was assessed with a st.. Read More»

Genet. Mol. Res. 10(4):
2011.December.14.4
DOI:
10.4238/2011.December.14.4
Animal Genetics   Research Article

Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers

Authors: X.N. Luo, M. Yang, X.F. Liang, K. Jin, L.Y. Lv, C.X. Tian, Y.C. Yuan and J. Sun

In this study, 12 polymorphic microsatellites were investigated to determine the genetic diversity and structure of 5 consecutive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breed.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.25.1
DOI:
http://dx.doi.org/10.4238/2015.September.25.1
Medical Genetics   Research Article

Genomic structure, polymorphism and expression analysis of growth hormone-releasing hormone and pituitary adenylate cyclase activating polypeptide genes in the half-smooth tongue sole (Cynoglossus semilaevis)

Authors: Q. Ma, S.F. Liu, Z.M. Zhuang, L. Lin, Z.Z. Sun, C.L. Liu, Y.Q. Su and Q.S. Tang

Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase activating polypeptide (PACAP) regulate development and somatic growth in teleosts; they may be associated with sexual growth dimorphism in the half-smooth tongue sole (Cynoglossus semilaevis). We found that the full-length GHRH and PACAP gene sequences ob.. Read More»

Genet. Mol. Res. 10(4):
2011.December.12.1
DOI:
10.4238/2011.December.12.1
Animal Genetics   Research Article

Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae)

Authors: S. Yang, L. Wang, Y. Zhang, X.C. Liu, H.R. Lin and Z.N. Meng

An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, .. Read More»

Genet. Mol. Res. 10(4):
2011.December.12.3
DOI:
10.4238/2011.December.12.3
Cytogenomics   Research Article

Worldwide diversity of the Y-chromosome tetra-local microsatellite DYS464

Authors: F.S.G. Kehdy and S.D.J. Pena

Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 r.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr968
DOI:
10.4238/vol9-3gmr968
Animal Genetics   Short communication

Development of microsatellite markers for Hoplias malabaricus (Erythrinidae)

Authors: S.G.C.A. Gondim, L.V. Resende, R.P.V. Brondani, R.G. Collevatti, N.J. Silva-J�ºnior, R.R. Pereira and M.P.C. Telles

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fo.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr877
DOI:
10.4238/vol9-3gmr877
Microbial Genetics   Research Article

Assessment of microsatellites in estimating inter- and intraspecific variation among Neotropical Crocodylus species

Authors: A. Bashyal, B.A. Gross, M. Venegas-Anaya, F. Lowrance and L.D. Densmore III

We tested microsatellites that were developed for the saltwater crocodile (Crocodylus porosus) for cross-species amplification and to provide an estimate of inter- and intraspecific variation among four species of Neotropical crocodiles (C. rhombifer, C. intermedius, C. acutus, and C. mo.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.2
DOI:
10.4238/2014.July.25.2
Animal Genetics   Research Article

Development of polymorphic expressed sequence tag-single sequence repeat markers in the common Chinese cuttlefish, Sepiella maindroni

Authors: R.H. Li, S.K. Lu, C.L. Zhang, W.W. Song, C.K. Mu and C.L. Wang

The common Chinese cuttlefish (Sepiella maindroni) is one of the popular edible cephalopod consumed across Asia. To facilitate the population genetic investigation of this species, we developed fourteen polymorphic microsatellite makers from expressed sequence tags of S. maindroni. The number of alleles at ea.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.3
DOI:
10.4238/2014.July.25.3
Animal Genetics   Research Article

Development of eight novel microsatellite markers for Huoyan geese

Authors: Z.Z. Cao, D. Su, Y.Y. Zhao, M. Liu, M. Gao and X.H. Luan

In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers we.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.10
DOI:
10.4238/2014.July.25.10