Authors: T.-J. Xu, Y.-N. Sun, Y.-T. Yuan, Z. Liao and R.-X. Wang
Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to sev.. Read More»
Authors: M. Moghbeli, O. Moaven, E. Dadkhah, M. Farzadnia, N.M. Roshan, H. Asadzadeh-Aghdaee, M.M. Bahar, R. Raeisossadati, M.M. Forghanifard, S.R.E.A. Bakhtiari, A. Baradaran and M.R. Abbaszadegan
Microsatellite instability in sporadic colorectal cancer patients was assessed, and the clinicopathological associations were evaluated in northeastern Iran, which is a high-risk region for gastrointestinal malignancies. Microsatellite instability (MSI)status of tumoral tissue, compared to normal tissue, was assessed with a st.. Read More»
Authors: X.N. Luo, M. Yang, X.F. Liang, K. Jin, L.Y. Lv, C.X. Tian, Y.C. Yuan and J. Sun
In this study, 12 polymorphic microsatellites were investigated to determine the genetic diversity and structure of 5 consecutive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breed.. Read More»
Authors: Q. Ma, S.F. Liu, Z.M. Zhuang, L. Lin, Z.Z. Sun, C.L. Liu, Y.Q. Su and Q.S. Tang
Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase activating polypeptide (PACAP) regulate development and somatic growth in teleosts; they may be associated with sexual growth dimorphism in the half-smooth tongue sole (Cynoglossus semilaevis). We found that the full-length GHRH and PACAP gene sequences ob.. Read More»
Authors: S. Yang, L. Wang, Y. Zhang, X.C. Liu, H.R. Lin and Z.N. Meng
An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, .. Read More»
Authors: F.S.G. Kehdy and S.D.J. Pena
Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 r.. Read More»
Authors: S.G.C.A. Gondim, L.V. Resende, R.P.V. Brondani, R.G. Collevatti, N.J. Silva-J�ºnior, R.R. Pereira and M.P.C. Telles
We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fo.. Read More»
Authors: A. Bashyal, B.A. Gross, M. Venegas-Anaya, F. Lowrance and L.D. Densmore III
We tested microsatellites that were developed for the saltwater crocodile (Crocodylus porosus) for cross-species amplification and to provide an estimate of inter- and intraspecific variation among four species of Neotropical crocodiles (C. rhombifer, C. intermedius, C. acutus, and C. mo.. Read More»
Authors: R.H. Li, S.K. Lu, C.L. Zhang, W.W. Song, C.K. Mu and C.L. Wang
The common Chinese cuttlefish (Sepiella maindroni) is one of the popular edible cephalopod consumed across Asia. To facilitate the population genetic investigation of this species, we developed fourteen polymorphic microsatellite makers from expressed sequence tags of S. maindroni. The number of alleles at ea.. Read More»
Authors: Z.Z. Cao, D. Su, Y.Y. Zhao, M. Liu, M. Gao and X.H. Luan
In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers we.. Read More»