Microsatellite
Development and characterization of polymorphic microsatellite markers in the gray mullet (Mugil cephalus)
Authors: T.-J. Xu, D.-Q. Sun, G. Shi and R.-X. Wang
Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and characterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from .. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr909
- DOI:
- 10.4238/vol9-3gmr909
Determination of ancestral proportions in synthetic bovine breeds using commonly employed microsatellite markers
Authors: H.M.S. Bicalho, C.G. Pimenta, I.K.P. Mendes, H.B. Pena, E.M. Queiroz and S.D.J. Pena
The International Society of Animal Genetics (ISAG) has chosen nine microsatellites (international marker set) as a standard that should be included in all cattle parentage studies. They are BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, and ETH225. We decided to ascertain whether this microsatellite set co.. Read More»
- Genet. Mol. Res. 5(3):
Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae)
Authors: Y. Wang, H. Lu, J. Zheng, K. Long, Y.H. Shi, Z.F. Gu and A.M. Wang
The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.21.5
- DOI:
- 10.4238/2011.December.21.5
Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae)
Authors: M.P.C. Telles, L.V. Resende, R.P.V. Brondani, R.G. Collevatti, M.C. Costa and N.J. Silva J�ºnior
We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr868
- DOI:
- 10.4238/vol9-3gmr868
Molecular analysis of apomixis in cassava
Authors: N.M.A. Nassar, D.C. Kalkmann and R. Collevatti
Cassava is the main staple for more than 800 million people in the tropics. It is propagated vegetatively by stem cuttings, which maintains superior genotypes but favors disease accumulation and spread. In this report, we present the results of the screening of the progeny and the second generation of the clone UnB 307 for apo.. Read More»
- Genet. Mol. Res. 5(3):
Characterization of 12 microsatellite loci for the Pacific lamprey, Entosphenus tridentatus (Petromyzontidae), and cross-amplification in five other lamprey species
Authors: E.K. Spice, T.A. Whitesel, C.T. McFarlane and M.F. Docker
The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish that is of conservation concern in North America and Asia. Data on Pacific lamprey population structure are scarce and conflicting, impeding conservation efforts. We optimized 12 polymorphic microsatellite loci for the Pacific lamprey. Three to 13 alleles per .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.22.2
- DOI:
- 10.4238/2011.December.22.2
Screening and characterization of new microsatellite markers in Fenneropenaeus penicillatus
Authors: J.B. Shangguan, Z.B. Li, Q.H. Li, G. Dai and Y.F. Ning
Fenneropenaeus penicillatus, with high protein and low fat, is a commercially important aquatic product in China. Microsatellite loci were developed according to the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Eight new polymorphic microsatellite markers for F. penicill.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.22
- DOI:
- 10.4238/2014.August.7.22
Development of novel simple sequence repeat markers from a genomic sequence survey database and their application for diversity assessment in Jatropha curcas germplasm from Guatemala
Authors: R.S. Raposo, I.G.B. Souza, M.E.C. Veloso, A.K. Kobayashi, B.G. Laviola and F.M. Diniz
The last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cul.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.25
- DOI:
- 10.4238/2014.August.7.25
Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology
Authors: L.M. Li, L. Wei, H.Y. Jiang, Y. Zhang, X.J. Zhang, L.H. Yuan and J.P. Chen
Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing techno.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.16
- DOI:
- 10.4238/2015.July.14.16
Polymorphic CA microsatellites in the third exon of the bovine BMP4 gene
Authors: X. Zhong, L.S. Zan, H.B. Wang and Y.F. Liu
We examined the variation of the BMP4 gene in four Chinese indigenous cattle breeds and investigated the association of this polymorphism with body measurement traits. Using PCR-SSCP and DNA sequencing, a polymorphic microsatellite was detected in the third exon of the bovine BMP4 gene in 459 samples from four Chinese indigeno.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr732
- DOI:
- 10.4238/vol9-2gmr732