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Microsatellite

Animal Genetics   Subspeciality Notes

Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae)

Authors: R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He

Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three .. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr806
DOI:
10.4238/vol9-2gmr806
Animal Genetics   Research Article

Insights about the genetic diversity and population structure of an offshore group of common bottlenose dolphins (Tursiops truncatus) in the Mid-Atlantic

Authors: C.S. Castilho, F. Pedone-Valdez, F. Bertuol, P. Fruet, R.C. Genoves, J.C. Di Tullio, G. Caon, L.S. Hoffmann and T.R.O. Freitas

Although the genus Tursiops has a worldwide distribution and is globally well-studied, some dolphin populations continue to face high risks of decline. Hence, it is necessary to assess the genetic diversity and structure of this genus to properly assess its conservation status and to implement appropriate management actions. In Brazil, genetic studies on .. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.15.2
DOI:
http://dx.doi.org/10.4238/2015.April.15.2
Animal Genetics   Research Article

Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes

Authors: P.A. Roratto, M.L. Bartholomei-Santos, A.M. Gutierrez, L. Kamenetzky, M.C. Rosenzvit and A. Zaha

Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobil.. Read More»

Genet. Mol. Res. 5(3):
Plant Genetics   Research Article

Screening and characterization of novel microsatellite loci in Lateolabrax japonicus

Authors: B.B. Li, Z.B. Li, J.B. Shangguan, Y.F. Ning, Y. Yuan, Y.S. Huang and X.Q. Mao

Lateolabrax japonicus, an economically important species, is widely consumed in the offshore coasts of China, Korea, and Japan. We identified 10 new L. japonicus microsatellite markers, using a modified protocol of fast isolation by AFLP of sequences containing repeats. Thirty L. japonicus individuals were collected from Xiame.. Read More»

Genet. Mol. Res. 15(4):
gmr15048378
DOI:
10.4238/gmr15048378
Human Genetics   Research Article

A combination of five short tandem repeats of chromosome 15 significantly improves the identification of Prader-Willi syndrome etiology in the Argentinean population

Authors: H.V. Ar�¡oz, M. Torrado, C. Barreiro and L. Chertkoff

Prader-Willi syndrome (PWS) is a multisystemic disorder caused by the loss of expression of paternally transcribed genes in the PWS critical region of chromosome 15. Various molecular mechanisms are known to lead to PWS: deletion 15q11-q13 (75% of cases), maternal uniparental disomy (matUPD15) (23%) and imprinting defects (2%).. Read More»

Genet. Mol. Res. 5(2):
Human Genetics   Research Article

Relationship of the methylenetetrahydrofolate reductase C677T polymorphism with microsatellite instability and promoter hypermethylation in sporadic colorectal cancer

Authors: Alessandra D. Clarizia, Luciana Bastos-Rodrigues, Helo�­sa B. Pena, Charles Anacleto, Benedito Rossi, Fernando A. Soares, Ademar Lopes, Jos�© Cl�¡udio C. Rocha, Ot�¡via Caballero, Anamaria Camargo, Andrew J.G. Simpson and S�©rgio D.J. Pena

The methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism is associated with the expression of a thermolabile enzyme with decreased activity that influences the pool of methyl-donor molecules. Several studies have reported an association between C677T polymorphism and susceptibility to colorectal cancer (CRC). Conside.. Read More»

Genet. Mol. Res. 5(2):
Plant Genetics   Research Article

Development and characterization of polymorphic microsatellite markers for Castanopsis hystrix (Fagaceae)

Authors: Y. Jiang, Z.H. Li, J.Y. Zhu and H.L. Liu

Castanopsis hystrix is one of the most important and dominant species in evergreen broad-leaved forests in subtropical China. However, the population of this species undergone severe decline because of deforestation over the past 2 decades. For both conservation and forestry management, it is essential to develop molecular markers for C. hystrix. We ident.. Read More»

Genet. Mol. Res. 14(1):
http://dx.doi.org/2015.March.30.1
DOI:
http://dx.doi.org/10.4238/2015.March.30.1
Plant Genetics   Research Article

Isolation and characterization of polymorphic microsatellite loci from aerial yam (Dioscorea bulbifera L.)

Authors: Q.Q. Yan, Y. Li, X.Q. Sun, J.L. Guo, Y.Y. Hang and M.M. Li

Dioscorea bulbifera L. is widely distributed in pantropical regions along the equator. The taxonomic treatment of this species is ambiguous due to its extreme polymorphic morphological characters. In order to provide tools to facilitate the study of genetic diversity, population structure, patterns of gene flow, and the mating.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.March.12.3
DOI:
http://dx.doi.org/10.4238/2014.March.12.3
Animal Genetics   Research Article

Fine-scale genetic structure patterns in two freshwater fish species, Geophagus brasiliensis (Osteichthyes, Cichlidae) and Astyanax altiparanae (Osteichthyes, Characidae) throughout a Neotropical stream

Authors: D.G. Ferreira1, S.C. Lima3, W. Frantine-Silva3, J.F. Silva1, C. Apolin�¡rio-Silva3, S.H. Sofia3, S. Carvalho2 and B.A. Galindo1

Streams are very important environments for Neotropical freshwater fish fauna, and possess a high number of species. These small drainages are also highlighted by their intrinsic biological and physicochemical features; however, knowledge on the genetic distribution of fish in these drainages is limited. Therefore, in the pres.. Read More»

Genet. Mol. Res. 15(4):
gmr15048124
DOI:
10.4238/gmr15048124
Plant Genetics   Research Article

Development and characterization of microsatellite loci in Excentrodendron hsienmu (Chun & How) H.T. Chang & R.H. Miau

Authors: W.H. Shen, Z.H. Li, Y.H. Peng, M.H. Yang, Z.Q. Tan and W. Zheng

Microsatellite markers were isolated using dual-suppression-PCR for the endangered species Excentrodendron hsienmu (Tiliaceae) to evaluate the genetic diversity and population structure of this species. A total of 11 polymorphic microsatellite loci were characterized in E. hsienmu. The number of alleles per locus ranged from 2 to 9, with an average of 5.2.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038060
DOI:
10.4238/gmr.15038060