Authors: R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He
Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three .. Read More»
Authors: C.S. Castilho, F. Pedone-Valdez, F. Bertuol, P. Fruet, R.C. Genoves, J.C. Di Tullio, G. Caon, L.S. Hoffmann and T.R.O. Freitas
Although the genus Tursiops has a worldwide distribution and is globally well-studied, some dolphin populations continue to face high risks of decline. Hence, it is necessary to assess the genetic diversity and structure of this genus to properly assess its conservation status and to implement appropriate management actions. In Brazil, genetic studies on .. Read More»
Authors: P.A. Roratto, M.L. Bartholomei-Santos, A.M. Gutierrez, L. Kamenetzky, M.C. Rosenzvit and A. Zaha
Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobil.. Read More»
Authors: B.B. Li, Z.B. Li, J.B. Shangguan, Y.F. Ning, Y. Yuan, Y.S. Huang and X.Q. Mao
Lateolabrax japonicus, an economically important species, is widely consumed in the offshore coasts of China, Korea, and Japan. We identified 10 new L. japonicus microsatellite markers, using a modified protocol of fast isolation by AFLP of sequences containing repeats. Thirty L. japonicus individuals were collected from Xiame.. Read More»
Authors: H.V. Ar�¡oz, M. Torrado, C. Barreiro and L. Chertkoff
Prader-Willi syndrome (PWS) is a multisystemic disorder caused by the loss of expression of paternally transcribed genes in the PWS critical region of chromosome 15. Various molecular mechanisms are known to lead to PWS: deletion 15q11-q13 (75% of cases), maternal uniparental disomy (matUPD15) (23%) and imprinting defects (2%).. Read More»
Authors: Alessandra D. Clarizia, Luciana Bastos-Rodrigues, HeloÃ?Âsa B. Pena, Charles Anacleto, Benedito Rossi, Fernando A. Soares, Ademar Lopes, JosÃ?© ClÃ?¡udio C. Rocha, OtÃ?¡via Caballero, Anamaria Camargo, Andrew J.G. Simpson and SÃ?©rgio D.J. Pena
The methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism is associated with the expression of a thermolabile enzyme with decreased activity that influences the pool of methyl-donor molecules. Several studies have reported an association between C677T polymorphism and susceptibility to colorectal cancer (CRC). Conside.. Read More»
Authors: Y. Jiang, Z.H. Li, J.Y. Zhu and H.L. Liu
Castanopsis hystrix is one of the most important and dominant species in evergreen broad-leaved forests in subtropical China. However, the population of this species undergone severe decline because of deforestation over the past 2 decades. For both conservation and forestry management, it is essential to develop molecular markers for C. hystrix. We ident.. Read More»
Authors: Q.Q. Yan, Y. Li, X.Q. Sun, J.L. Guo, Y.Y. Hang and M.M. Li
Dioscorea bulbifera L. is widely distributed in pantropical regions along the equator. The taxonomic treatment of this species is ambiguous due to its extreme polymorphic morphological characters. In order to provide tools to facilitate the study of genetic diversity, population structure, patterns of gene flow, and the mating.. Read More»
Authors: D.G. Ferreira1, S.C. Lima3, W. Frantine-Silva3, J.F. Silva1, C. Apolin�¡rio-Silva3, S.H. Sofia3, S. Carvalho2 and B.A. Galindo1
Streams are very important environments for Neotropical freshwater fish fauna, and possess a high number of species. These small drainages are also highlighted by their intrinsic biological and physicochemical features; however, knowledge on the genetic distribution of fish in these drainages is limited. Therefore, in the pres.. Read More»
Authors: W.H. Shen, Z.H. Li, Y.H. Peng, M.H. Yang, Z.Q. Tan and W. Zheng
Microsatellite markers were isolated using dual-suppression-PCR for the endangered species Excentrodendron hsienmu (Tiliaceae) to evaluate the genetic diversity and population structure of this species. A total of 11 polymorphic microsatellite loci were characterized in E. hsienmu. The number of alleles per locus ranged from 2 to 9, with an average of 5.2.. Read More»