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Microsatellite

Plant Genetics   Research Article

Isolation and characterization of microsatellite loci for the herbaceous tuber crop, Amorphophallus konjac (Araceae)

Authors: C. Pan Y.N. You Y. Diao Z.L. Hu J.M. Chen

Amorphophallus konjac is an herbaceous tuber crop with tremendous potential for commercial development. We report the development of microsatellite primers for this important crop species. Thirteen polymorphic microsatellite markers were developed and tested in two populations of A. konjac from the Wuling Mountain Region (WL p.. Read More»

Genet. Mol. Res. 11(4):
2012.October.17.8
DOI:
10.4238/2012.October.17.8
Animal Genetics   Research Article

Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus

Authors: G. Chen, S. Huang, J. Gao, X. Bai, W. Wang and X. Cao

The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenB.. Read More»

Genet. Mol. Res. 14(2):
2015.May.29.1
DOI:
10.4238/2015.May.29.1
Microbial Genetics   Research Article

Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata

Authors: Y.F. Ning, Z.B. Li, J.B. Shangguan, Y.S. Huang, P.D. Jiang, A.L. Xu and Y. Yuan

The pen shell, Atrina pectinata, is an economically valuable species that is widely distributed along the coastal waters of temperate and tropical areas, mainly growing in the Indian and Pacific Oceans. Eight novel microsatellite loci from the genome of A. pectinata were developed using fast isolation by amplified fragment length polymorphism of sequence .. Read More»

Genet. Mol. Res. 14(3):
2015.July.27.17
DOI:
10.4238/2015.July.27.17
Plant Genetics   Research Article

Genetic diversity among melon accessions (Cucumis melo) from Turkey based on SSR markers

Authors: Y.A. Ka�§ar O. Simsek I. Solmaz N. Sari Y.Y. Mendi

Melon (Cucumis melo) is an important vegetable crop in Turkey, where it is grown in many regions; the most widely planted lines are local winter types belonging to the var. inodorous. We examined 81 melon genotypes collected from different provinces of Turkey, compared with 15 reference melon genotypes obtained from INRA/France, to determine genetic diversit.. Read More»

Genet. Mol. Res. 11(4):
2012.November.29.2
DOI:
10.4238/2012.November.29.2
Microbial Genetics   Research Article

Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae)

Authors: R.O. Manoel, M.L.M. Freitas, E.V. Tambarussi, J. Cambuim, M.L.T. Moraes and A.M. Sebbenn

Genipa americana is a tropical tree species that is widely distributed in the humid tropical and subtropical regions of Central and South America. This study investigated Mendelian inheritance, genetic linkage, and genotypic disequilibrium at six microsatellite loci developed for G. americana. Adult trees (188) and regenerants (163) were sampled and genot.. Read More»

Genet. Mol. Res. 14(3):
2015.July.27.4
DOI:
10.4238/2015.July.27.4
Animal Genetics   Research Article

Microsatellite analysis of genetic diversity and genetic structure in five consecutive breeding generations of mandarin fish Siniperca chuatsi (Basilewsky)

Authors: T.L. Yi, W.J. Guo, X.F. Liang, M. Yang, L.Y. Lv, C.X. Tian, Y. Song, C. Zhao and J. Sun

In this report, 10 polymorphic microsatellites were applied to assess the genetic diversity and genetic differentiation of 5 consecutive breeding generations of mandarin fish, Siniperca chuatsi (Basilewsky). The results from total number of alleles, average polymorphism information content, and average homozygosity and heterozygosity showed that the genet.. Read More»

Genet. Mol. Res. 14(1):
http://dx.doi.org/2015.March.30.19
DOI:
http://dx.doi.org/10.4238/2015.March.30.19
Medical Genetics   Research Article

Development of polymorphic microsatellite loci in Odontobutis obscura using Illumina paired-end sequencing and the test of cross-species amplification

Authors: H.-G. Bae, J. An, J. Jun, S.-M. Kweon, S. Kim, J.-N. Yu, B.-J. Kim, H. Sakai, Y.J. Lee and H.Y. Suk

Odontobutis obscura is a bottom-dwelling freshwater fish native to East Asia. Its range encompasses southwest China, western Japan, and Geoje Island in South Korea. Despite its widespread range in China and Japan, only a small and spatially isolated population is found in South Korea. We developed a total of 23 novel and polym.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038405
DOI:
10.4238/gmr.15038405
Microbial Genetics   Research Article

Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae)

Authors: H. Li, Z.-S. Zhou, J. Guo and L.H. Lin

We isolated and characterizated 12 polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae). The loci were screened in 37 E. velox individuals. The number of alleles ranged from 6 to 16. The observed heterozygosity ranged from 0.432 to 0.919, and the expected heterozygosity ranged from 0.685 to 0.902. These microsatelli.. Read More»

Genet. Mol. Res. 11(4):
2012.October.9.5
DOI:
10.4238/2012.October.9.5
Cytogenomics   Research Article

Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites

Authors: I.G. Hamoy, E.J.M. Santos and S.E.B. Santos

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtain.. Read More»

Genet. Mol. Res. 7(1):
vol7-1gmr394
DOI:
10.4238/vol7-1gmr394
Animal Genetics   Short communication

Isolation and characterization of tetranucleotide repeat polymorphic microsatellite loci in Larus saundersi (Aves, Laridae)

Authors: H.-X. Jiang, S.L. Ren, Y.-Q. Hou, M. Yang and X.-B. Wu

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscl.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1164
DOI:
10.4238/vol10-3gmr1164