Microsatellite
Molecular characterization of the Andean blackberry, Rubus glaucus, using SSR markers
Authors: M. Marulanda, A.M. L�³pez and M. Uribe
The species Rubus glaucus, also known as the Andean or “Castilla” blackberry, is one of nine edible species of this genus that grow naturally in Central and South America. In Colombia, this species is the most important of all Rubus species for agricultural and commercial purposes. We used 20 SSRs developed for oth.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.10.3
- DOI:
- 10.4238/2012.February.10.3
Isolation and characterization of novel microsatellite markers in commercial selected golden Malaysian arowana fish, Scleropages formosus (Osteoglossidae)
Authors: B. Manoharan, Z. Sulaimen, F. Omar, R.Y. Othman, S.Z. Mohamed and S. Bhassu
Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr944
- DOI:
- 10.4238/vol10-2gmr944
Polymorphisms of the cocaine-amphetamine-regulated transcript (CART) gene and their association with reproductive traits in Chinese goats
Authors: P.Q. Wang, L.M. Deng, B.Y. Zhang, M.X. Chu and J.Z. Hou
Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorp.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1091
- DOI:
- 10.4238/vol10-2gmr1091
Development of polymorphic microsatellites for Sillago sihama based on next-generation sequencing and transferability to Sillago japonica
Authors: R.X. Wu, H.R. Zhang, S.F. Niu, Y. Zhai and X.F. Liu
Sillago sihama (Forsskål, 1775), a commercially important marine fishery species in the Indo-West Pacific, is being developed as a target species for aquaculture and stock enhancement in China. However, due to the limitations of traditional isolation methods, the available microsatellite loci, or simple sequence repeats .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049046
- DOI:
- 10.4238/gmr15049046
Polymorphisms of three new microsatellite sites of the dystrophin gene
Authors: R.F. Sun, Y.S. Zhu, J.L. Feng, Z. Tian, W.J. Kuang, Y. Liu, H.B. Zhang and S.B. Li
To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr962
- DOI:
- 10.4238/vol10-2gmr962
Polymorphic microsatellite loci for the Japanese anchovy Engraulis japonicus (Engraulidae)
Authors: L. Lin, L. Zhu, S.-F. Liu, Y.-Q. Su and Z.-M. Zhuang
We isolated and characterized 14 polymorphic microsatellite loci in the Japanese anchovy (Engraulis japonicus) using a (GT)13-enriched genomic library. The numbers of alleles per locus ranged from 6 to 31, with a mean of 17.8. The observed and expected heterozygosities ranged from 0.180 to 0.949 and from 0.172 to 0.966, with means of 0.731 and 0.825, resp.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1085
- DOI:
- 10.4238/vol10-2gmr1085
Development and characterization of 70 novel microsatellite markers for the sea cucumber (Apostichopus japonicus)
Authors: W. Peng, Z.M. Bao, H.X. Du, J.J. Yan, L.L. Zhang and J.J. Hu
The sea cucumber (Apostichopus japonicus) is an important item in Asian cuisine. It is currently produced through aquaculture, especially in China, after being overexploited in the wild in the 1990s. We isolated 70 novel polymorphic microsatellite loci using an enrichment-colony hybridization protocol. All loci were characteri.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.24.2
- DOI:
- 10.4238/2012.February.24.2
Isolation and characterization of polymorphic microsatellite loci from the invasive plant Solidago canadensis (Asteraceae)
Authors: S.-Y. Zhao, S.-G. Sun, Y.-H. Guo, J.-M. Chen and Q.-F. Wang
Solidago canadensis, a clonal herb originally from North America (common name: Canada goldenrod), is an invasive species in many countries. We developed microsatellite primers for this species. Eleven polymorphic loci were generated and primers were designed. Polymorphism of these 11 loci was assessed in 35 plants from two populations (Wuhan and Shanghai).. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.17.4
- DOI:
- 10.4238/2012.February.17.4
Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor
Authors: Y. Wang, F. Wang, Y.H. Shi, Z.F. Gu, A.M. Wang
The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of allel.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1182
- DOI:
- 10.4238/vol10-2gmr1182
Characterization of 10 novel microsatellite loci for the brown marbled grouper, Epinephelus fuscoguttatus (Serranidae)
Authors: M.A.A. Mokhtar, M.N. Normah, S.V. Kumar and S.N. Baharum
Epinephelus fuscoguttatus is a commercially important marine fish species in southeast Asia. Due to overfishing and water pollution, this species has been declared as near-threatened. Thus, to provide information to help maintain and preserve the species, microsatellites were developed, using an enriched genomic library method.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1117
- DOI:
- 10.4238/vol10-2gmr1117