Microsatellite
Isolation and characterization of new polymorphic microsatellite markers from the cuttlefish Sepiella maindroni (Cephalopoda; Sepiidae)
Authors: B.-Y. Guo, P.Z. Qi, A.Y. Zhu, Z.M. Lv, W.C. Wang and C.W. Wu
Fifteen new polymorphic microsatellite loci were developed for the cuttlefish Sepiella maindroni. In 32 individuals from a wild population of coastal Ningde, Fujian Province, China, the number of alleles at these loci varied between 2 and 12, with an average of 5.86. The mean observed and expected heterozygosities were 0.6917 and 0.5993, respectively. Amo.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.11.1
- DOI:
- 10.4238/2013.July.11.1
Recycling of superfine resolution agarose gel
Authors: T.-Y. Seng, R. Singh, Q.Z. Faridah, S.-G. Tan and S.S.R.S. Alwee
Genetic markers are now routinely used in a wide range of applications, from forensic DNA analysis to marker-assisted plant and animal breeding. The usual practice in such work is to extract the DNA, prime the markers of interest, and sift them out by electrically driving them through an appropriate matrix, usually a gel. The gels, made from polyacrylamid.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.March.11.1
- DOI:
- 10.4238/2013.March.11.1
Characterization and application of newly developed polymorphic microsatellite markers in the Ezo red fox (Vulpes vulpes schrencki)
Authors: T. Tada, Y. Seki, Y. Kameyama, Y. Kikkawa and K. Wada
The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite ma.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049104
- DOI:
- 10.4238/gmr15049104
Development and characterization of microsatellite loci in a threatened marine fish, Cheilinus undulatus (humphead wrasse)
Authors: J. Hu, X.P. Zhu, J. Luo, S.W. Yin, Y.H. Peng, Y.L. Hu and F. Zhu
Cheilinus undulatus (humphead wrasse) is a marine fish distributed widely throughout the tropical Indo-Pacific. It has been listed as vulnerable in the IUCN Red Data Book and in CITES Appendix II four times. Fifteen microsatellite loci were isolated and characterized for this species. The number of alleles ranged from 3 to 15 per locus, and the observed a.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.2
- DOI:
- 10.4238/2013.July.30.2
Transferability of microsatellite markers among economically and ecologically important galliform birds
Authors: N. Bech, C. Novoa, J.F. Allienne and J. Boissier
We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even .. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr760
- DOI:
- 10.4238/vol9-2gmr760
Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)
Authors: Q.H. Li, Z.B. Li, G. Dai, X.J. Chen, L.N. Chen, Y.Y. Cao, J.B. Shangguan and Y.F. Ning
Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were us.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.12
- DOI:
- 10.4238/2013.July.30.12
Development of microsatellite markers for Mytilus coruscus (Mytilidae), an economically important mussel in the East China Sea
Authors: B.-Y. Guo, C.-W. Wu, C.-L. Liu, A.-Y. Zhu and Y.-Y. Ye
Twelve new polymorphic microsatellite loci were developed for the hard-shelled mussel, Mytilus coruscus. In 32 individuals from a wild population of coastal Zhoushan, Zhejiang Province, China, the number of alleles at these loci varied from 3 to 15, with a mean of 5.667. The mean observed and expected heterozygosities were 0.6927 and 0.6591, respectively... Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.5
- DOI:
- 10.4238/2013.July.30.5
Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda
Authors: S.W. Jia, P. Liu, J. Li, J.T. Li and L.Q. Pan
The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.8.1
- DOI:
- 10.4238/2013.August.8.1
Novel tetranucleotide microsatellite markers for Chinese beard eel (Cirrhimuraena chinensis Kaup)
Authors: H. Li, T.M. Ge, S.X. Ding and H.J. Wang
In this study, we characterized 12 novel polymorphic microsatellite loci for Chinese beard eel (Cirrhimuraena chinensis Kaup) from a tetranucleotide microsatellite-enriched library. Loci screened on a sample of 37 individuals from Xiamen wild stocks revealed 8 to 24 alleles per locus, with a mean of 13.83 over all loci. Observed and expected heterozygosit.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.2.3
- DOI:
- 10.4238/2013.August.2.3
Population structure and genetic diversity of maize inbreds derived from tropical hybrids
Authors: E.C.M. Lanes, J.M.S. Viana, G.P. Paes, M.F.B. Paula, C. Maia, E.T. Caixeta and G.V. Miranda
The objectives of this study were to identify the population structure and to assess the genetic diversity of maize inbreds. We genotyped 81 microsatellite loci of 90 maize inbreds that were derived from tropical hybrids and populations. The population structure analysis was based on a Bayesian approach. Each subpopulation was.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.2
- DOI:
- 10.4238/2014.September.12.2