Microsatellite
Novel polymorphic microsatellite markers in Odontobutis potamophila
Authors: Y.B. Shen, D. Li, J.L. Li, R.Q. Wang and Y.F. Xuan
We characterized 16 novel polymorphic loci isolated from a partial genomic DNA library of Odontobutis potamophila enriched for CA repeats. We tested the variability of these microsatellites on 51 unrelated individuals collected in China. All loci were polymorphic. The average allele number was 14.6 per locus, ranging from 2 to 27. The observed heterozygos.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.9.13
- DOI:
- 10.4238/2015.June.9.13
Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)
Authors: S.-L. Liu, Z.-M. Gu, Y.-Y. Jia, J.-L. Zhao, W.-P. Jiang, Q. Li and F. Li
The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)12 and (GA)12 probes. Th.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.14
- DOI:
- 10.4238/2014.September.12.14
Inverted migration of rare whisker sheatfish in Nong-Han Lake, northeastern Thailand: Implications for conservation
Authors: P. Phongkaew, U. Arunyawat, A. Swatdipong and V. Hongtrakul
Nong-Han Lake, Thailand, sustains the whisker sheatfish (Micronema bleekeri Günther, 1864), which is a rare species of freshwater catfish. Wild-caught whisker sheatfish has been intensively harvested to meet market demand; yet, genetic information about this species remains unknown. To assist with the in situ.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.16
- DOI:
- 10.4238/2014.September.12.16
Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)
Authors: Z.B. Li, Q.H. Li, J.B. Shangguan, Y.F. Ning and G. Dai
Lunella coronata granulata, from the family Turbinidae, is an economically important species. The first set of 10 polymorphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.6
- DOI:
- 10.4238/2015.June.11.6
Isolation and characterization of microsatellite markers in Atrina vexillum Born
Authors: Y.F. Ning, Z.B. Li, Y.S. Huang, X.Q. Mao, B.B. Li, Y. Yuan and J.B. Shangguan
Atrina vexillum Born is an economically valuable species, widely distributed in the coastal waters of temperate and tropical areas of the Asia Pacific region. Twenty one novel microsatellite loci were identified in the genome of A. vexillum Born using the protocol for fast isolation by amplified fragment length polymorphism of.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027204
- DOI:
- 10.4238/gmr.15027204
Development of polymorphic microsatellite loci for a new fish species, Chinese sillago (Sillago sinica)
Authors: N. Song, L.Y. Wang, L.N. Yin, Z.Q. Han and T.X. Gao
Sillago sinica is a newly identified species belonging to Sillaginidae, Perciforms, and was found along the coast of China in 2011. In the present study, 81 microsatellite loci were isolated from an enriched genomic library, and 24 positive clones containing microsatellite repeats had adequate flanking sequences for the develo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027212
- DOI:
- 10.4238/gmr.15027212
Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing
Authors: H. Liu, Z.C. Long, L.N. Li, Q.F. Wang, J.M. Chen and K. Liao
To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The numbe.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027886
- DOI:
- 10.4238/gmr.15027886
Cross-species amplification and polymorphism of microsatellite loci in Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) in Brazilian cropping systems
Authors: N.A. Leite, A.S. Corr�ªa, A. Alves-Pereira, J.B. Campos, M.I. Zucchi and C. Omoto
The Old World bollworm Helicoverpa armigera (Hübner) was recently discovered in Brazil. This species is closely related to the New World bollworm H. zea (Boddie), and mating between these species has already been reported under laboratory conditions. Here, we tested the cross-species amplification of 20 microsatellite (SS.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027890
- DOI:
- 10.4238/gmr.15027890
Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines
Authors: A.A. Leal, C.A. Mangolin, A.T. do Amaral J�ºnior, L.S.A. Gon�§alves, C.A. Scapim, A.S. Mott, I.B.O. Eloi, V. Cordov�©s and M.F.P. da Silva
Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr692
- DOI:
- 10.4238/vol9-1gmr692
Microsatellite usefulness is independent of phylogenetic distance in Tyrant flycatchers (Aves: Tyrannidae): a test using two globally threatened species
Authors: B. Mahler, A.R.R. Schneider, A.S. Di Giacomo, A.G. Di Giacomo, J.C. Reboreda and R. Tiedemann
Tyrant flycatchers (Aves: Tyrannidae) are endemic to the New World, and many species of this group are threatened or near-threatened at the global level. The aim of this study was to test the 18 microsatellite markers that have been published for other Tyrant flycatchers in the Strange-tailed Tyrant (Alectrurus risora) and the Sharp-tailed Tyrant (Culiciv.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.12
- DOI:
- 10.4238/2013.August.12.12