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Microsatellite

Human Genetics   Research Article

Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species

Authors: P.F. Cruz, J.S. Batista, K.M. Formiga, M.S. Rafael, W.P. Tadei and J.M.M. Santos

Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity rang.. Read More»

Genet. Mol. Res. 12(3):
2013.March.13.11
DOI:
10.4238/2013.March.13.11
Cytogenomics   Research Article

Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)

Authors: M.P.C. Telles, J.B. Silva, L.V. Resende, R.P. Vianello, L.J. Chaves, T.N. Soares and R.G. Collevatti

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localitie.. Read More»

Genet. Mol. Res. 12(3):
2013.February.6.3
DOI:
10.4238/2013.February.6.3
Animal Genetics   Research Article

Transferability of microsatellite loci from exotic Cervidae to Brazilian brocket deer (Mazama spp, Mammalia: Cervidae)

Authors: A.M.B. Mantellatto, E.A.G. Carnelossi and J.M.B. Duarte

Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were test.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr720
DOI:
10.4238/vol9-1gmr720
Cytogenomics   Research Article

Interpretation of electrophoretograms of seven microsatellite loci to determine the genetic diversity of the Arabian Oryx

Authors: I.A. Arif, H.A. Khan, M. Shobrak, A.A. Al Homaidan, M. Al Sadoon, A.H. Al Farhan and A.H. Bahkali

Microsatellite markers are commonly used for examining population structure, especially inbreeding, outbreeding and gene flow. An array of microsatellite loci, preferably with multiallelic presentation, is preferable for ensuring accurate results. However, artifact peaks or stutters in the electrophoretograms significantly ham.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr714
DOI:
10.4238/vol9-1gmr714
Medical Genetics   Research Article

Isolation and characterization of novel microsatellite markers in Mercenaria mercenaria

Authors: Y.F. Ning, Z.B. Li, X.Q. Mao, B.B. Li, Y.S. Huang, Y. Yuan and J.B. Shangguan

Mercenaria mercenaria, also known as the hard clam, is widely distributed in the coastal waters of temperate and tropical areas in the Asian Pacific region. This species is widely popular in the international market, especially in the United States, Europe, and other Western countries, because of its high protein value, taste,.. Read More»

Genet. Mol. Res. 14(1):
gmr.15017497
DOI:
10.4238/gmr.15017497
Animal Genetics   Short communication

Polyandry in the red-headed river turtle Podocnemis erythrocephala (Testudines, Podocnemididae) in the Brazilian Amazon

Authors: C. Fantin, I.P. Farias, L.A.S. Monjel�³ and T. Hrbek

The genus Podocnemis comprises six living species, including P. erythrocephala (irapuca - red-headed river turtle). Data are available concerning the reproductive biology of the species of the genus Podocnemis, but little is known about their reproductive strategies. Considering the total lack of such data for P. erythrocephal.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr749
DOI:
10.4238/vol9-1gmr749
Bioinformatics   Research Article

Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest

Authors: M. Novello, J.F. Rodrigues, F. Pinheiro, G.C.X. Oliveira, E.A. Veasey and S. Koehler

Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci .. Read More»

Genet. Mol. Res. 12(3):
2013.September.3.3
DOI:
10.4238/2013.September.3.3
Plant Genetics   Research Article

Isolation and characterization of novel microsatellites for Abies koreana and A. nephrolepis (Pinaceae)

Authors: J.K. Hong, J. Lim, B.Y. Lee and M. Kwak

Abies koreana is an endemic and rare species from Korea and is classified as endangered by the International Union for Conservation of Nature. Although the genetic diversity assessment for current population of A. koreana needs to be performed urgently, no microsatellite markers have been developed for this species. In the pre.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027542
DOI:
10.4238/gmr.15027542
Cytogenomics   Research Article

Genetic variability in Melipona scutellaris from Rec�´ncavo, Bahia, Brazil

Authors: J.L. Viana, A.K. Francisco, C.A.L. Carvalho and A.M. Waldschmidt

Bees play a key role in pollination and thereby help maintain plant diversity. The stingless bee Melipona scutellaris is an important pollinator in northeastern Brazil because it is endemic to this region. Both deforestation and timber harvesting have reduced the nesting sites for this species, thus reducing its population and range. Genetic studies may h.. Read More»

Genet. Mol. Res. 12(3):
2013.September.10.1
DOI:
10.4238/2013.September.10.1
Bioinformatics   Research Article

Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species

Authors: Q.-Q. Yan, X.-Q. Sun, J.-L. Guo, Y.-Y. Hang and M.-M. Li

Dioscorea zingiberensis C.H. Wright (Dioscoreaceae) is an endemic species in central and southwestern China. In order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression PCR technique. The number of alleles per locus ranged from 3 to 21, with an average o.. Read More»

Genet. Mol. Res. 12(3):
2013.September.19.10
DOI:
10.4238/2013.September.19.10