Microsatellite
Development and cross-species transferability of unigene-derived microsatellite markers in an edible oil woody plant, Camellia oleifera (Theaceae)
Authors: B.G. Jia, Q. Lin, Y.Z. Feng, X.Y. Hu, X.F. Tan, F.G. Shao and L. Zhang
Camellia oleifera is an important edible oil woody plant in China. Lack of useful molecular markers hinders current genetic research on this tree species. Transcriptome sequencing of developing C. oleifera seeds generated 69,798 unigenes. A total of 6949 putative microsatellites were discovered among 6042 SSRcontaining unigenes. Then, 150 simple sequence .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.33
- DOI:
- 10.4238/2015.June.18.33
Development of novel polymorphic microsatellite markers for the blood clam Tegillarca granosa by pyrosequencing
Authors: Y.H. Dong, H.H. Yao, S.F. Shi, Y.B. Bao and Z.H. Lin
Large amounts of expressed sequence tags (ESTs) generated using next-generation sequencing technologies provide a cost-effective and valuable genomic resource for the development of microsatellite markers. In this study, we isolated 115 novel polymorphic microsatellite markers for the blood clam Tegillarca granosa from ESTs in 454 sequencing data. All the.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.6
- DOI:
- 10.4238/2015.August.7.6
Verification and characterization of chromosome duplication in haploid maize
Authors: E.G. de Oliveira Couto, E.V. Resende Von Pinho, R.G. Von Pinho, A.D. Veiga, M.R. de Carvalho, F. de Oliveira Bustamante and M.S. Nascimento
Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.9
- DOI:
- 10.4238/2015.June.26.9
Paeonia (Paeoniaceae) expressed sequence tag-derived microsatellite markers transferred to Paeonia delavayi
Authors: D.X. Wang, H. Ma, Y.L. Zhang, A.A. Duan, W.J. Li and Z.H. Li
Paeonia Franchet contains approximately 30 species of perennial herbs and is the only member of the family Paeoniaceae. Paeonia delavayi is an endangered ornamental plant that has a compound flower color and is endemic to southwest China. Its optimal habitats have been fragmented and its population size has greatly decreased as a result of human activitie.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.6
- DOI:
- 10.4238/2013.April.17.6
First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers
Authors: H.Y. Ma, S.J. Li, N.N. Feng, C.Y. Ma, W. Wang, W. Chen and L.B. Ma
The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 trip.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026929
- DOI:
- 10.4238/gmr.15026929
Isolation and characterization of microsatellite markers in Ruditapes philippinarum
Authors: Z.B. Li, Y.F. Ning, J.B. Shangguan, B.B. Li, X.Q. Mao, Y.S. Huang and Y. Yuan
Ruditapes philippinarum is considered a commercially valuable species, which is commonly found in tidal flats along West Pacific coasts. In China, it is mainly distributed in the southeast sea. In this study, 16 novel microsatellite loci from the R. philippinarum genome were developed, using the protocol of fast isolation by a.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027742
- DOI:
- 10.4238/gmr.15027742
Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers
Authors: R.F. Peixoto-Junior, S. Creste, M.G.A. Landell, D.S. Nunes, A. Sanguino, M.F. Campos, R. Vencovsky, E.V. Tambarussi and A. Figueira
Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the gen.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.23
- DOI:
- 10.4238/2014.September.26.23
Isolation and characterization of simple sequence repeat markers for the herbaceous species Phyla scaberrima (Verbenaceae)
Authors: C.L. Chaves, E.A. Ruas, C.F. Ruas, J. Delfini, N.C. Bejatto, B.D. G�³es and P.M. Ruas
Phyla scaberrima (Verbenaceae) is a herbaceous species distributed from Mexico to Panama. Because of its well-known sweet properties and other medicinal uses, this species is cultivated in South America and the Caribbean. Phyla scaberrima has been arbitrarily extracted from nature, resulting in a severe reduc.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.24
- DOI:
- 10.4238/2014.September.26.24
Genetic diversity in fragmented populations of Populus talassica inferred from microsatellites: implications for conservation
Authors: X.H. Zhu, S.P. Cheng, T. Liao and X.Y. Kang
Populus talassica Kom. is an ecologically important species endemic to central Asia. In China, its main distribution is restricted to the Ili region in the Xinjiang Autonomous Region. An understanding of genetic diversity and population structure is crucial for the development of a feasible conservation strategy. Twenty-six hi.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027899
- DOI:
- 10.4238/gmr.15027899
Selected representative microsatellite loci for genetic monitoring and population structure analysis of miniature swine
Authors: C. Wang,L.L. Xu,Y.H. Wu,X.Y. Du,X.Y. Huo,J. Gao,Z.K. Li,Y.C. Li and Z.W. Chen
To optimize the combination of microsatellite loci for genetic control of outbred swine stocks, 32 of 100 loci distributed among almost all chromosomes (except 12) were screened out by 1.5% agarose, 8% polyacrylamide gel and capillary electrophoresis scanning among 3 miniature swine outbred stocks, namely Bama (BM), Guizhou (GZ) and Tibeta (TB). The mean .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.5
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.5