Microsatellite
Development of novel microsatellite markers for conservation genetic studies of Vulpes vulpes (Canidae) by using next-generation sequencing method
Authors: J.-N. Yu,C.-U. Chung,K.H. Oh,B.-K. Lee and C.E. Lim
The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles) .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.12
Isolation and characterization of polymorphic microsatellite markers for blue fox (Alopex lagopus)
Authors: Y.M. Li, P.C. Guo, J.Y. Lu, C.Y. Bai, Z.H. Zhao and S.Q. Yan
The blue fox, belonging to the family Canidae, is a coat color variant of the native arctic fox (Alopex lagopus). To date, microsatellite loci in blue fox are typically amplified using canine simple sequence repeat primers. In the present study, we constructed an (AC)n enrichment library, and isolated and identified 17 polymor.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027990
- DOI:
- 10.4238/gmr.15027990
New polymorphic microsatellite loci of threadsail filefish, Stephanolepis cirrhifer (Teleostei, Monacanthidae), from Korean waters
Authors: C.-M. An, H.S. An, J.W. Lee and S.W. Hong
The threadsail filefish, Stephanolepis cirrhifer (Monacanthidae), is found mainly in the western Pacific. It is intensively caught in Korea and is a highly appreciated seafood delicacy. Consequently, the natural population of this species has drastically decreased, despite introductions from hatcheries. To provide information necessary for its conservatio.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.14.8
- DOI:
- 10.4238/2013.May.14.8
Isolation and characterization of microsatellite markers for Osmanthus fragrans (Oleaceae) using 454 sequencing technology
Authors: W.J. Yuan, Y. Li, Y.F. Ma, Y.J. Han and F.D. Shang
Osmanthus fragrans (Oleaceae) is an evergreen shrub or small tree that grows in south China. In this study, Roche 454 FLX+ sequencing combined with the magnetic bead enrichment method was used to isolate microsatellite markers from the genome of O. fragrans. A total of 1471 microsatellites that contained enough flanking sequences for primer pair design we.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.15
- DOI:
- 10.4238/2015.December.16.15
Development and characterization of microsatellite loci for genetic studies of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)
Authors: V.A.C. Pavinato, K.L. Silva-Brand�£o, M. Monteiro, M.I. Zucchi, J.B. Pinheiro, F.L.F. Dias and C. Omoto
We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on averag.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.14.3
- DOI:
- 10.4238/2013.May.14.3
Genetic diversity in wild sweet cherries (Prunus avium) in Turkey revealed by SSR markers
Authors: S. Ercisli, G. Agar, N. Yildirim, B. Duralija, A. Vokurka and H. Karlidag
Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the num.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1196
- DOI:
- 10.4238/vol10-2gmr1196
Candidate gene region for control of rib eye area in Canchim beef cattle
Authors: S.L. Meirelles, G.V. Gouveia, G. Gasparin, M.M. Alencar, J.J.S. Gouveia and L.C.A. Regitano
Investigation of molecular marker effects on production traits is essential to define marker assisted selection strategies in beef cattle. We looked for a possible association of molecular markers and backfat thickness (BFT) and rib eye area (REA) in Canchim (5/8 Charolais + 3/8 Zebu) and MA (offspring of Charolais bulls and 1.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1175
- DOI:
- 10.4238/vol10-2gmr1175
Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus
Authors: P.-Z. Qi, C.-X. Xie, B.-Y. Guo, C.-W. Wu, S.-M. Lu and Y.-J. Duan
Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polym.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.21.7
- DOI:
- 10.4238/2013.May.21.7
Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi
Authors: C.F. Sun, X. Ye, Y.Y. Tian and J.J. Dong
In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild populat.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.15
- DOI:
- 10.4238/2015.August.10.15
Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea)
Authors: X.Q. Wang, D. Zeng, X. Ma, G. Xiong, Z.Y. Wang and T. Sakamoto
The large yellow croaker (Larimichthys crocea) is one of the largest marine net-cage cultured species in the oceans around China. In the present study, we isolated and characterized 13 polymorphic microsatellite markers from genomic libraries of L. crocea. Loci were screened for 10 wild specimens from 2 sites in southeast of China. All loci were polymorph.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.7
- DOI:
- 10.4238/2015.August.14.7