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Microsatellite

Bioinformatics   Research Article

Isolation and characterization of eight novel microsatellite markers in Acanthopagrus schlegelii

Authors: X.Q. Mao, Z.B. Li, Y. Yuan, Y.F. Ning, J.B. Shangguan,Y.S. Huang, M Yang and B.B. Li

Acanthopagrus schlegelii is a warm temperate demersal fish, which inhabits the sediment substrate or rocky reefs in shallow seas. As this fish is a nutritionally endowed species with good palatability, it is a highly valuable commercial species for aquaculture and has a long historical standing in Western Pacific countries. Be.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027902
DOI:
10.4238/gmr.15027902
Human Genetics   Research Article

Structure and genetic diversity of natural Brazilian pepper populations (Schinus terebinthifolius Raddi)

Authors: S.V. ?lvares-Carvalho, J.F. Duarte, T.C. Santos, R.M. Santos, R. Silva-Mann and D. Carvalho

In the face of a possible loss of genetic diversity in plants due the environmental changes, actions to ensure the genetic variability are an urgent necessity. The extraction of Brazilian pepper fruits is a cause of concern because it results in the lack of seeds in soil, hindering its distribution in space and time. It is imp.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028123
DOI:
10.4238/gmr.15028123
Human Genetics   Research Article

Development and characterization of microsatellite markers of the eastern keelback mullet (Liza affinis)

Authors: L. Liu, T.X. Gao, Z.Q. Han, C.H. Li, D.R. Sun and N. Song

Twenty-four polymorphic microsatellite loci were isolated and characterized for Liza affinis using a (GT)13-enriched genomic library. The number of alleles per locus ranged from 3 to 9, with a mean number of 6.250. The observed and expected heterozygosities ranged from 0.417 to 1.000 and from 0.550 to 0.861, with an average of.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028380
DOI:
10.4238/gmr.15028380
Bioinformatics   Short communication

Development and characterization of microsatellite markers for the walking goby (Scartelaos viridis; Gobiidae)

Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang

Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1015
DOI:
10.4238/vol10-1gmr1015
Animal Genetics   Research Article

Genetic parameters and mapping quantitative trait loci associated with tibia traits in broilers

Authors: B.N.N. Ragognetti, N.B. Stafuzza, T.B.R. Silva, T.C.S. Chud, N.V. Grupioni, V.A.R. Cruz, J.O. Peixoto, K. Nones, M.C. Ledur and D.P. Munari

Selection among broilers for performance traits is resulting in locomotion problems and bone disorders, once skeletal structure is not strong enough to support body weight in broilers with high growth rates. In this study, genetic parameters were estimated for body weight at 42 days of age (BW42), and tibia traits (length, width, and weight) in a populati.. Read More»

Genet. Mol. Res. 14(4):
2015.December.21.27
DOI:
10.4238/2015.December.21.27
Bioinformatics   Short communication

Isolation and characterization of 12 polymorphic microsatellite markers for the frog Pelophylax hubeiensis

Authors: J.-C. Yan, F.-H. Zhu and H.-L. Wu

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to .. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1003
DOI:
10.4238/vol10-1gmr1003
Human Genetics   Research Article

Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing

Authors: A.W. Gichira, Z.C. Long, G.W. Hu, R.W. Gituru, Q.F. Wang and J.M. Chen

Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individu.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028168
DOI:
10.4238/gmr.15028168
Plant Genetics   Short communication

Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae)

Authors: C.L. Ma, H.L. Wu, H.Y. Hu, X. Wu, G.C. Ma, Y.G. Fu and Z.Q. Peng

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the po.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1008
DOI:
10.4238/vol10-1gmr1008
Plant Genetics   Research Article

Genetic characterization of papaya plants (Carica papaya L.) derived from the first backcross generation

Authors: H.C.C. Ramos, M.G. Pereira, F.F. Silva, L.S.A. Gon?§alves, F.O. Pinto, G.A. de Souza Filho and T.S.N. Pereira

The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time req.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1004
DOI:
10.4238/vol10-1gmr1004
Plant Genetics   Research Article

Isolation and characterization of 16 microsatellite loci in marble goby (Oxyeleotris marmoratus)

Authors: J. Luo, X.P. Zhu, Y.H. Peng and S.W. Yin

A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.03.. Read More»

Genet. Mol. Res. 12(2):
2013.January.4.2
DOI:
10.4238/2013.January.4.2