Authors: X.Q. Mao, Z.B. Li, Y. Yuan, Y.F. Ning, J.B. Shangguan,Y.S. Huang, M Yang and B.B. Li
Acanthopagrus schlegelii is a warm temperate demersal fish, which inhabits the sediment substrate or rocky reefs in shallow seas. As this fish is a nutritionally endowed species with good palatability, it is a highly valuable commercial species for aquaculture and has a long historical standing in Western Pacific countries. Be.. Read More»
Authors: S.V. ?lvares-Carvalho, J.F. Duarte, T.C. Santos, R.M. Santos, R. Silva-Mann and D. Carvalho
In the face of a possible loss of genetic diversity in plants due the environmental changes, actions to ensure the genetic variability are an urgent necessity. The extraction of Brazilian pepper fruits is a cause of concern because it results in the lack of seeds in soil, hindering its distribution in space and time. It is imp.. Read More»
Authors: L. Liu, T.X. Gao, Z.Q. Han, C.H. Li, D.R. Sun and N. Song
Twenty-four polymorphic microsatellite loci were isolated and characterized for Liza affinis using a (GT)13-enriched genomic library. The number of alleles per locus ranged from 3 to 9, with a mean number of 6.250. The observed and expected heterozygosities ranged from 0.417 to 1.000 and from 0.550 to 0.861, with an average of.. Read More»
Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang
Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences.. Read More»
Authors: B.N.N. Ragognetti, N.B. Stafuzza, T.B.R. Silva, T.C.S. Chud, N.V. Grupioni, V.A.R. Cruz, J.O. Peixoto, K. Nones, M.C. Ledur and D.P. Munari
Selection among broilers for performance traits is resulting in locomotion problems and bone disorders, once skeletal structure is not strong enough to support body weight in broilers with high growth rates. In this study, genetic parameters were estimated for body weight at 42 days of age (BW42), and tibia traits (length, width, and weight) in a populati.. Read More»
Authors: J.-C. Yan, F.-H. Zhu and H.-L. Wu
Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to .. Read More»
Authors: A.W. Gichira, Z.C. Long, G.W. Hu, R.W. Gituru, Q.F. Wang and J.M. Chen
Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individu.. Read More»
Authors: C.L. Ma, H.L. Wu, H.Y. Hu, X. Wu, G.C. Ma, Y.G. Fu and Z.Q. Peng
Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the po.. Read More»
Authors: H.C.C. Ramos, M.G. Pereira, F.F. Silva, L.S.A. Gon?§alves, F.O. Pinto, G.A. de Souza Filho and T.S.N. Pereira
The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time req.. Read More»
Authors: J. Luo, X.P. Zhu, Y.H. Peng and S.W. Yin
A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.03.. Read More»