Microsatellite
Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes
Authors: Y.-J. Xu, M.-J. Liao, Y.-G. Wang, Z.-C. Liu, P. Qin, Z. Zhang, X.-J. Rong and X.-Z. Liu
We investigated the genetic diversity of the southern flounder Paralichthys lethostigma. Microsatellite-enriched libraries were constructed and novel microsatellite markers were developed and applied for genetic detection of wild populations. Cross-species amplification was also conducted in five pleuronectiforme species. Of 45 randomly selected and seque.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.16.2
- DOI:
- 10.4238/2013.December.16.2
Characterization of nine microsatellite loci for the tree species Parapiptadenia rigida (Fabaceae-Mimosoideae) and their transferability
Authors: B.F. Costa, L.A. Rodrigues, E.A. Ruas, L.B. Souza, C.F. Ruas, B.G. Vieira, A.R.O. Conson and P.M. Ruas
Parapiptadenia rigida, locally known as angico, is a tropical tree common in the semideciduous Brazilian forest. Its wood is naturally resistant to insect attack and is useful for construction. Extracts from the tree have medicinal properties. We characterized nine microsatellite loci for P. rigida. Thirty-five alleles were detected in a sample of 45 indi.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.7
- DOI:
- 10.4238/2012.August.13.7
Isolation and characterization of microsatellite loci in hybrid giant tiger grouper
Authors: Y.S. Huang, Z.B. Li, Y.F. Ning, J.B. Shangguan, Y. Yuan, X.Q. Mao and B.B. Li
The hybrid giant tiger grouper is a fish that has considerable commercial value and has become increasingly important for aquaculture in South East Asia since 2008. In order to prevent any reduction in genetic diversity in hybrid grouper as a result of aquaculture, we have identified 21 microsatellite markers that can be used .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.35
- DOI:
- 10.4238/2015.November.18.35
Genetic structure in fragmented populations of Solanum lycocarpum A. St.-Hil. with distinct anthropogenic histories in a Cerrado region of Brazil
Authors: T.M. Moura, K. Martins, P.S. Sujii, A.M. Sebbenn and L.J. Chaves
Solanum lycocarpum is a woody tree widely distributed in the Cerrado that reaches high population densities in disturbed environments. We examined the genetic diversity and population differentiation of six S. lycocarpum populations with different degrees of human disturbance in order to determine if they are negatively affected by anthropogenic activity... Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.16
- DOI:
- 10.4238/2012.July.10.16
Genetic diversity and population structure of Chinese pony breeds using microsatellite markers
Authors: L.X. Xu, S.L. Yang, R.Y. Lin, H.B. Yang, A.P. Li and Q.S. Wan
China is one of the principal origins of ponies in the world. We made a comprehensive analysis of genetic diversity and population structure of Chinese ponies based on 174 animals of five indigenous Chinese pony breeds from five provinces using 13 microsatellite markers. One hundred and forty-four alleles were detected; the mean number of effective allele.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.25.4
- DOI:
- 10.4238/2012.June.25.4
Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species
Authors: Q.H. Gu, Q.Q. Cheng, X.J. Li and C.J. Zhou
Bellamya is a widely distributed freshwater snail genus in China; however, its genetic diversity is completely unknown. Sixty-five novel microsatellite loci were isolated and characterized from a microsatellite-enriched library of Bellamya aeruginosa genomic DNA. Most of the 65 loci were successfully amplified. We fou.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.8
- DOI:
- 10.4238/2015.November.25.8
Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin
Authors: Y.-X. Liu, C.-Y. Ma, H.-Y. Ma, C.-L. Feng, S.-J. Li and L.-B. Ma
Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5'-anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity p.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.8
- DOI:
- 10.4238/2015.November.30.8
Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers
Authors: L. Wei1, W.W. Shao1, H.B. Zhou2, J. Ping2, L.M. Li3 and Y.P. Zhang2
welve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus. We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36). The mean number of observed alleles per locus was 7.3 (range 4 to 13). Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively. One loc.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.32
- DOI:
- 10.4238/2015.October.29.32
Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites
Authors: C. Wang1,2*, S.J. Zhang1*, X.Y. Du1, Y.M. Xu3, X.Y. Huo1, L.F. Liao3 and Z.W. Chen1
The grey hamster has been used in biomedical research for decades. However, effective molecular methods for evaluating the genetic structure of this species are lacking, which hinders its wider usage. In this study, we employed cross-amplification of microsatellite loci of species within the same genus by polymerase chain reaction. Loci screened included .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.19
- DOI:
- 10.4238/2015.November.13.19
Nineteen polymorphic microsatellite markers developed for Trachinotus ovatus
Authors: Z.Z. Xie, M.W. Huang, W. Xu, C. Peng, J.N. He, Z.N. Meng, Y. Zhang, S.S. Li and H.R. Lin
To evaluate the population genetic diversity of the ovate pompano, we isolated and characterized 19 microsatellite markers using a (CA)13-enriched genomic library. Polymorphism was assessed in 30 individuals from a single population collected from the Daya Bay Aquaculture Center, Guangdong, China. The number of alleles per locus ranged from 2 to 18 with a.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.13
- DOI:
- 10.4238/2014.December.12.13