Microsatellite
Contribution of microsatellites markers in the clarification of the origin, genetic risk factors, and implications for conservation of Tunisian native sheep breeds
Authors: Y. Ben Sassi-Zaidy F. Maretto F. Charfi-Cheikhrouha A. Mohamed-Brahmi M. Cassandro
The genetic diversity and genetic relationship of the two main groups of African sheep, thin-tailed and fat-tailed sheep, represented by the indigenous Tunisian sheep breeds “Barbarine” (BAR, fat-tailed) and “Queue Fine de l’Ouest” (QFO, thin-tailed) were investigated. The genotypes of 110 animals belonging to these two breed.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017059
- DOI:
- 10.4238/gmr.15017059
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)
Authors: M.L. Oliveira J.M.B. Duarte
We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20'S, 47°17'W), with the help of a detection dog; then, stored in ethanol and g.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.16.8
- DOI:
- 10.4238/2013.January.16.8
Cross-species amplification of selected zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations
Authors: D. Kaczmarczyk
Fifteen sets of PCR primers designed for the amplification of microsatellite loci from Danio rerio and Phoxinus neogaeus Campostoma DNA that have been proven applicable for molecular studies of several species of cyprinids were tested for amplification of microsatellites from lake minnow DNA. The samples were taken from 298 fish inhabiting 6 distinct popu.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.7
- DOI:
- 10.4238/2013.January.24.7
Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing
Authors: L.A. Fusinatto S. Lopes A. Silva-Ferreira J. Alexandrino C.F.B. Haddad C.F.D. Rocha F. Sequeira
The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.15
- DOI:
- 10.4238/2013.January.24.15
Genetic variability and heterogeneity of Venezuelan equine encephalitis virus vector Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations of the Colombian Atlantic coast, based on microsatellite loci
Authors: F. Bello and V. Becerra
In Colombia, the mosquito Ochlerotatus taeniorhynchus has been identified as an efficient vector of the epidemic-epizootic Venezuelan equine encephalitis virus. We evaluated the genetic variability and heterogeneity of this mosquito in Colombian populations using eight microsatellite DNA loci. Two hundred and te.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr652
- DOI:
- 10.4238/vol8-3gmr652
Isolation and characterization of novel polymorphic microsatellite markers for Epinephelus akaara
Authors: Z.Z. Xie, L.Y. Zheng, L. Tang, Z.J. Tang, S.S. Li, Y. Zhang and H.R. Lin
In the present study, ten novel microsatellite markers were developed from an enriched-(CA)13 genomic library of Epinephelus akaara. The mean number of alleles per locus was 21.6, with a range of 12 to 33. Observed heterozygosity ranged from 0.767 to 0.967, and expected heterozygosity ranged from 0.831 to 0.975, with mean values of 0.877 and 0.923, respec.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.28
- DOI:
- 10.4238/2015.October.28.28
Characterization of eight novel microsatellite markers in the green-lipped mussel Perna viridis (Mytilidae)
Authors: Y.Y. Cao Z.B. Li Q.H. Li X.J. Chen L. Chen G. Dai
The green lipped mussel, also known as the Asian green mussel (Perna viridis) is a fast reproducing and valuable food source, but it is also considered an invasive species and can clog and damage pipes and marine equipment. Eight novel polymorphic microsatellite loci for P. viridis were isolated and characterized. Microsatellite polymorphism was evaluated.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.4
- DOI:
- 10.4238/2013.February.7.4
Polymorphic microsatellite loci for Japanese Spanish mackerel (Scomberomorus niphonius)
Authors: L. Lin, L. Zhu, S.-F. Liu, Q.-S. Tang, Y.-Q. Su and Z.-M. Zhuang
We isolated and characterized 21 polymorphic microsatellite loci in Japanese Spanish mackerel (Scomberomorus niphonius) using a (GT)13-enriched genomic library. Forty individuals were collected from Qingdao, China. We found 3 to 24 alleles per locus, with a mean of 8.8. The observed and expected heterozygosities ranged from 0... Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.8.2
- DOI:
- 10.4238/2012.May.8.2
Chloroplast diversity of Casearia grandiflora in the Cerrado of Piau� State
Authors: M.F. Costa, A.A. Pereira, J.B. Pinheiro, M.I. Zucchi, A.S.F. Ara�ºjo, R.L.F. Gomes, S.E.S. Valente, M.E.A. Oliveira and A.C.A. Lopes
Casearia grandiflora (Salicaceae) is a typical Cerrado species adapted to disturbed environments, making it useful for restoration projects. Knowledge of genetic diversity is important for establishing conservation strategies for this species. This study aimed to compare chloroplast haplotype diversity and structure of C. gran.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019572
- DOI:
- 10.4238/gmr16019572
Characterization of microsatellite markers in cassava based on microsatellite-AFLP technique
Authors: S. Whankaew, S. Sraphet, R. Thaikert, D.R. Smith and K. Triwitayakorn
We developed molecular markers for cassava based on the microsatellite-amplified fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were developed and used for the analysis of 48 samples of Manihot species, consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthagine.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.2
- DOI:
- 10.4238/2012.May.15.2