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Microsatellites

Animal Genetics   Research Article

Development and characterization of microsatellite molecular markers for the eye mask frog Batrachyla taeniata (Girard, 1855)

Authors: Ingrid Cisternas-Medina, Juan Carlos Ortiz and Sandra Ferrada-Fuentes

Batrachyla taeniata (Batrachylidae) is a small frog from Chile and Argentina with a distributional range that spans more than 1500 km. However, their populations are threatened due to the change in land use, deforestation, and human settlements. Through massive sequencing runs in ION Torrent PGM, was built DNA fragment library, from which we fina.. Read More»

Genet. Mol. Res. 18(2):
Plant Genetics   Research Article

Isolation and cross-amplification of fifteen microsatellites for oil-rewarding Calceolaria species (Calceolariaceae)

Authors: M. Mur�ºa, G. Peralta, L. Carrasco, and F. P�©rez

Calceolaria is a diversified American genus whose species are mainly visited by oil-collecting bees, representing a highly specialized plant-pollinator system. However, although this is a unique plant system, different aspects of its ecology and evolution remain unexplored. In this study we characterized fifteen polymorphic mi.. Read More»

Genet. Mol. Res. 17(1):
gmr16039863
DOI:
10.4238/gmr16039863
Plant Genetics   Research Article

Genetic diversity analysis in Malaysian giant prawns using expressed sequence tag microsatellite markers for stock improvement program

Authors: K.H. Atin, A. Christianus, N. Fatin, A.C. Lutas, M. Shabanimofrad and B. Subha

The Malaysian giant prawn is among the most commonly cultured species of the genus Macrobrachium. Stocks of giant prawns from four rivers in Peninsular Malaysia have been used for aquaculture over the past 25 years, which has led to repeated harvesting, restocking, and transplantation between rivers. Consequently, a stock impr.. Read More»

Genet. Mol. Res. 16(3):
gmr16035685
DOI:
10.4238/gmr16035685
Plant Genetics   Research Article

Farmer variety exchange along Amazonian rivers influences the genetic structure of manioc maintained in a regional Brazilian Gene Bank

Authors: S.B. Sousa, G.F. Silva, M.C. Dias, C.R. Clement and N.R. Sousa

Knowledge of agro-morphological and genetic variability of germplasm diversity is a prerequisite for its use in breeding. The aim of this study was to characterize the genetic diversity of the manioc (Manihot esculenta) Active Germplasm Bank (AGB) at Embrapa Amazônia Ocidental using microsatellite markers. We evaluated 4.. Read More»

Genet. Mol. Res. 16(3):
gmr16039690
DOI:
10.4238/gmr16039690
Plant Genetics   Research Article

Genetic variability in banana diploids and nonparametric statistics of fragments associated with natural fruit finger drop

Authors: M.A. Rodrigues, E.P. Amorim, C.F. Ferreira, C.A.S. Ledo and J.R.F. Santana

Natural fruit finger drop in bananas is one of the major concerns of producers as well as consumers. The aim of this study was to estimate the genetic diversity among 15 diploid bananas with different levels of resistance to finger drop (RFD) using SSR and ISSR (inter-simple sequence repeat) markers, and assess the possibility.. Read More»

Genet. Mol. Res. 16(3):
gmr16039554
DOI:
10.4238/gmr16039554
Plant Genetics   Research Article

Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers

Authors: S.I.C. Carvalho, L.B. Bianchetti, C.F. Ragassi, C.S.C. Ribeiro, F.J.B. Reifschneider, G.S.C. Buso and F.G. Faleiro

Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morp.. Read More»

Genet. Mol. Res. 16(3):
gmr16039689
DOI:
10.4238/gmr16039689
Plant Genetics   Research Article

Assessment of allelic diversity among drought-resistant cotton genotypes using microsatellite markers

Authors: A. Javaid, F.S. Awan, F.M. Azhar and I.A. Khan

Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (S.. Read More»

Genet. Mol. Res. 16(2):
gmr16029664
DOI:
10.4238/gmr16029664
Animal Genetics   Research Article

Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer

Authors: J.C. Mar�­n, P. Orozco-terWengel, K. Romero, J.P. V�¡squez, V. Varas and J.A. Vianna

Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully i.. Read More»

Genet. Mol. Res. 13(2):
2014.April.25.4
DOI:
10.4238/2014.April.25.4
Animal Genetics   Research Article

Cross-amplification of nonspecific micro-satellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer

Authors: J.C. Mar�­n, P. Orozco-terWengel, K. Romero, J.P. V�¡squez, V. Varas and J.A. Vianna

Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully i.. Read More»

Genet. Mol. Res. 13(2):
2014.April.25.4
DOI:
10.4238/2014.April.25.4
Animal Genetics   Research Article

Genetic diversity and population structure of different varieties of Morada Nova hair sheep from Brazil

Authors: J.S.B. Ferreira, S.R. Paiva, E.C. Silva, C.M. McManus, A.R. Caetano, D.A.E. Fa�§anha and M.A.N. de Sousa

The aim of this study was to analyze genetic diversity and population structure among varieties of White (N = 40), Red (N = 32), and Black (N = 31) Morada Nova hair sheep from flocks in the northeastern Brazilian semiarid region. Fifteen nuclear microsatellite markers and two regions of mitochondrial DNA were used. The intra-p.. Read More»

Genet. Mol. Res. 13(2):
2014.April.3.20
DOI:
10.4238/2014.April.3.20