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Molecular Marker

Plant Genetics   Research Article

Genetic relationships among Heliconia (Heliconiaceae) species based on RAPD markers

Authors: L.P. Marouelli, P.W. Inglis, M.A. Ferreira and G.S.C. Buso

The family Heliconiaceae contains a single genus, Heliconia, with approximately 180 species of Neotropical origin. This genus was formerly allocated to the family Musaceae, but today forms its own family, in the order Zingiberales. The combination of inverted flowers, a single staminode and drupe fruits is an exclusive charact.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr847
DOI:
10.4238/vol9-3gmr847
Plant Genetics   Research Article

RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil

Authors: I.G.B. Souza, S.E.S. Valente, F.B. Britto, V.A.B. de Souza and P.S.C. Lima

We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from the USA and one from India. Genomic DNA, extracted from leaf material usi.. Read More»

Genet. Mol. Res. 10(4):
2011.December.14.1
DOI:
10.4238/2011.December.14.1
Animal Genetics   Research Article

Development of eight novel microsatellite markers for Huoyan geese

Authors: Z.Z. Cao, D. Su, Y.Y. Zhao, M. Liu, M. Gao and X.H. Luan

In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers we.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.10
DOI:
10.4238/2014.July.25.10
Medical Genetics   Short communication

Comparison of methods for estimates of molecular genetic diversity in genus Croton: influence of coefficients, clustering strategies and data projection

Authors: M.M. Scaldaferri, J.S. Freitas, J.G.P. Vieira, Z.S. Gon�§alves, A.M. Souza and C.B.M. Cerqueira-Silva

We investigated 10 similarity (and disimilarity) coefficients in a set of 40 wild genotypes of Croton linearifolius subjected to analyses using hierarchical grouping methods, grouping methods by optimization and data projection in two-dimensional space. Genotypes were characterized by analyzing DNA polymorphism with t.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.11
DOI:
10.4238/2014.July.25.11
Microbial Genetics   Research Article

Genetic diversity analysis of tree peony germplasm using iPBS markers

Authors: Y.B. Duan, D.L. Guo, L.L. Guo, D.F. Wei and X.G. Hou

We examined the genetic diversity of 10 wild species (populations) and 55 varieties of tree peony using inter-primer binding site (iPBS) markers. From a total of 36 iPBS primers, 16 were selected based on polymorphic amplification. The number of bands amplified by each primer ranged from 9 to 19, with an average of 12.88 bands per primer. The length of ba.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.31
DOI:
10.4238/2015.July.3.31
Plant Genetics   Research Article

Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species

Authors: S.Q. Xuan, J.Y. Zheng, H. Wang, P. Gao, S.B. Zhou, K. Liu and G.-P. Zhu

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs usin.. Read More»

Genet. Mol. Res. 10(4):
2011.December.19.2
DOI:
10.4238/2011.December.19.2
Plant Genetics   Research Article

Molecular characterization of Ephedra species found in Pakistan

Authors: S. Ghafoor, M.M. Shah, H. Ahmad, Z.A. Swati, S.H. Shah, A. Pervez and U. Farooq

Ephedra, also known as “ma huang”, is a dioecious, drought- and frost-resistant, perennial, evergreen shrub with compelling medicinal value. The genus is represented by 42 species around the world, 9 of which were provisionally reported from Pakistan. Species of the genus have a controversial taxonomy due to their .. Read More»

Genet. Mol. Res. 6(4):
Animal Genetics   Research Article

Genetic structure in Brazilian breeding colonies of the Roseate Spoonbill (Platalea ajaja, Aves: Threskiornithidae)

Authors: Mino CI and Del Lama SN

Roseate Spoonbills (Platalea ajaja, Linnaeus) are wading birds present in two of the most important Brazilian wetlands: the Pantanal wetlands and Rio Grande do Sul marshes. Natural populations of these species have not been previously studied with variable nuclear molecular markers. In order to support decision making regardin.. Read More»

Genet. Mol. Res. 6(2):
Human Genetics   Research Article

Association study of c.910A>G and c.1686C>G polymorphisms in XRCC1 gene with risk of hepatocellular carcinoma in the Chinese population

Authors: W.F. Xia, X.P. Ma, X.R. Li, H. Dong and J.L. Yi

XRCC1 (human X-ray repair complementing defective repair in Chinese hamster cell 1) gene is considered a potentially important gene influencing the risk of hepatocellular carcinoma (HCC). Our analyses detected two allelic variants of XRCC1, c.910A>G and c.1686C>G. We aimed to investigate whether these polymorphisms influ.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.February.28.3
DOI:
http://dx.doi.org/10.4238/2014.February.28.3
Medical Genetics   Research Article

Molecular cytogenetic analysis of a ring-Y infertile male patient

Authors: Carvalho FM, Wolfgramm EV, Degasperi I, Verbeno BM, Vianna BA, Chagas FF, Perroni AMS, Paula F and Louro ID

In the present study, we report on the case of a 43-yearold male patient seeking for fertility assistance, who showed a seminal analysis and testicular biopsy of complete azoospermia. Peripheral blood culture for chromosome studies revealed a karyotype of 46 chromosomes with a ring-Y-chromosome that lost the long arm heterochr.. Read More»

Genet. Mol. Res. 6(1):