Molecular Marker
Development and characterization of polymorphic microsatellite markers in the gray mullet (Mugil cephalus)
Authors: T.-J. Xu, D.-Q. Sun, G. Shi and R.-X. Wang
Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and characterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from .. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr909
- DOI:
- 10.4238/vol9-3gmr909
Estimation of genetic diversity in a natural population of cambui tree (Myrciaria tenella O. Berg) using ISSR markers
Authors: J.G.S. Santana, A.L.S. Nascimento, T.S. Costa, T.M.B. de Almeida, A.R.C. Rabbani and A.V.C. Silva
Cambui (Myrciaria tenella O. Berg) is a native species from Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of genetic diversity is fundame.. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048819
- DOI:
- 10.4238/gmr.15048819
Association of BoLA-DRB3.2 alleles with tick (Boophilus microplus) resistance in cattle
Authors: M.L. Martinez, M.A. Machado, C.S. Nascimento, M.V.G.B. Silva, R.L. Teodoro, J. Furlong, M.C.A. Prata, A.L. Campos, M.F.M. Guimar?£es, A.L.S. Azevedo, M.F.A. Pires and R.S. Verneque
Losses caused by bovine tick burdens in tropical countries have a tremendous economic impact on production systems. Besides reducing production, this parasite can cause death in the most susceptible animals. The use of commercial acaricides has been the major method of control, but their misuse has led to tick resistance to ma.. Read More»
- Genet. Mol. Res. 5(3):
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers
Authors: ?.M. Vidal L.J. Vieira, C.F. Ferreira, F.V.D. Souza, A.S. Souza and C.A.S. Ledo
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.2
- DOI:
- 10.4238/2015.July.14.2
Molecular identification of Greek olive (Olea europaea) cultivars based on microsatellite loci
Authors: K. Roubos, M. Moustakas and F.A. Aravanopoulos
Olea europaea is one of the oldest species of domesticated trees. We used microsatellite markers for fingerprinting and for evaluation of genetic similarity and structure of 26 Greek olive cultivars, which cover most of the olive cultivation regions of Greece, including previously undescribed denominations from northern Greece.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr916
- DOI:
- 10.4238/vol9-3gmr916
Analysis of â??Fujiâ?? apple somatic variants from next-generation sequencing
Authors: H.S. Lee1, G.H. Kim1, S.I. Kwon2, J.H. Kim2, Y.S. Kwon2 and C. Choi1
The domesticated apple (Malus x domestica Borkh.) is a major fruit crop of temperate regions of the world. ‘Fuji’ apple (Ralls Genet x Delicious), a famous apple cultivar in Korea, has been very popular since its promotion in Japan in 1958. ‘Fuji’ and its bud mutant cultivars possess variable levels of genetic diversity. Nonetheles.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038185
- DOI:
- 10.4238/gmr.15038185
Identification and evaluation of polymorphisms in FABP3 and FABP4 in beef cattle
Authors: I.M.Z. Blecha, F. Siqueira, A.B.R. Ferreira, G.L.D. Feij?³, R.A.A. Torres Junior, S.R. Medeiros2, I.I. Sousa, G.G. Santiago and A.L.J. Ferraz
Single nucleotide polymorphisms (SNPs) were screened in FABP3 and FABP4 by automatic sequencing of pools of DNA from crossbred animals whose phenotypes belonged to the upper and lower extremes for back fat and marbling, as well as of a pool of DNA from sires used for crossbreeding. Five SNPs were identified in FABP3 and another nine SNPs were identified i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Genetic diversity and relatedness between Canind?© and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers
Authors: T.S. C?¢mara, J.F. Nunes, F.M. Diniz, G.R. Silva, A.M. Ara?ºjo
The aim of this study was to access the genetic diversity and relatedness between Canind?© and British Alpine goat breeds in the States of Piau? and Cear?¡ using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype .. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019569
- DOI:
- 10.4238/gmr16019569
Genetic dissection of agronomic traits in Capsicum baccatum var. pendulum
Authors: M.M. Moulin, R. Rodrigues, C.S. Bento, L.S.A. Gon?§alves,J.O. Santos, C.P. Sudr?© and A.P. Viana
Genetic mapping is very useful for dissecting complex agronomic traits. Genetic mapping allows for identification of quantitative trait loci (QTL), provide knowledge on a gene position and its adjacent region, and enable prediction of evolutionary mechanisms, in addition to contributing to synteny studies. The aim of this study was to predict genetic valu.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.23
- DOI:
- 10.4238/2015.March.20.23
Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays)
Authors: E.C. Oliveira, A.T. Amaral J?ºnior, L.S.A. Gon?§alves, G.F. Pena, S.P. Freitas J?ºnior, R.M. Ribeiro and M.G. Pereira
We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize h.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr767
- DOI:
- 10.4238/vol9-2gmr767