Molecular Marker
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Par�¡ State using ISSR markers
Authors: S.M. Rodrigues, E.F. Moura, G.K.S. Ramos and M.S.P. Oliveira
Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. D.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048887
- DOI:
- 10.4238/gmr15048887
Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae)
Authors: R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He
Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three .. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr806
- DOI:
- 10.4238/vol9-2gmr806
Genome wide selection in Citrus breeding
Authors: I.B. Gois, A. Bor�©m, M. Cristofani-Yaly, M.D.V. de Resende, C.F. Azevedo, M. Bastianel, V.M. Novelli2and M.A. Machado
Genome wide selection (GWS) is essential for the genetic improvement of perennial species such as Citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. This study assessed GWS efficiency in a population of Citrus and compared it with selecti.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048863
- DOI:
- 10.4238/gmr15048863
Prediction of maize single-cross performance by mixed linear models with microsatellite marker information
Authors: M. Balestre, R.G. Von Pinho and J.C. Souza
We evaluated the potential of the best linear unbiased predictor (BLUP) along with the relationship coefficient for predicting the performance of untested maize single-cross hybrids. Ninety S0:2 progenies arising from three single-cross hybrids were used. The 90 progenies were genotyped with 25 microsatellite markers, with nin.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr791
- DOI:
- 10.4238/vol9-2gmr791
Genetic variability of Mahanarva sp (Hemiptera: Cercopidae) collected from different sites in Brazil
Authors: A.M. Auad, R. Domingues, M.A. Machado, L.S. Souza, G.S. Carvalho and S.V. Paula-Moraes
Spittlebugs are the leading cause of damage to tall grasses. Annual losses are estimated to reach 2.1 billion dollars in sugarcane crops and grazing land throughout the world. Correct identification of these species is difficult due to similarities in color, body size and male genitalia. Molecular markers have been useful in t.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr794
- DOI:
- 10.4238/vol9-2gmr794
Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers
Authors: P.P. Batista, J.F. Santos, N.T. Oliveira, A.P.D. Pires, C.M.S. Motta and E.A. Luna-Alves Lima
The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr422
- DOI:
- 10.4238/vol7-3gmr422
Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst
Authors: M.A. Khan, J.L. Cheng, Z.Q. Mei, C.L. Wei and J.J. Fu
Development of sequence-characterized amplified region (SCAR) markers from random-amplified polymorphic DNA (RAPD) fragments is a valuable molecular approach for the genetic identification of different species. By using SCAR markers, molecular analysis is reduced to a simple polymerase chain reaction (PCR) analysis using primers designed from the amplicon.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038536
- DOI:
- 10.4238/gmr.15038536
Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean
Authors: J. Ma, S.C. Guan, D. Yao, Y.F. Wei and P.W. Wang
Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products. We.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1366
- DOI:
- 10.4238/vol10-3gmr1366
Breeding cassava for apomixis
Authors: Nagib M.A. Nassar and Rosane G. Collevatti
Apomixis genes have been successfully transferred to cassava (Manihot esculenta) by hybridizing it with the wild species, M. glaziovii. The interspecific hybrid of cassava and M. glaziovii was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were g.. Read More»
- Genet. Mol. Res. 4(4):
Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae)
Authors: T.J. Xu, D.Q. Sun, H.Y. Li and R.X. Wang
The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1045
- DOI:
- 10.4238/vol10-3gmr1045