Molecular Marker
Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis
Authors: E.C.M. Lanes, C. Nick, K.N. Kuki, R.D. Freitas and S.Y. Motoike
Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethyl.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.9
- DOI:
- 10.4238/2013.September.23.9
Initial assessment of natural diversity in Mexican fig landraces
Authors: M.T. Garc�?a-Ruiz, V.M. Mendoza-Castillo, E. Valadez-Moctezuma and A. Muratalla-L�?ºa
The common fig (Ficus carica L.) was introduced into Mexico by Spanish Franciscan missionaries in the 16th century. It is widely assumed that Mexican figs are the Spanish cultivar Black Mission. We collected and propagated 12 fig plants from six landraces from different states in Central Mexico that represent different climate. All of them were grown in a.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.12
- DOI:
- 10.4238/2013.September.23.12
Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses
Authors: A.C. Leme, M.R.R. Bevilaqua, S.A. Rhoden, C.A. Mangolin, M.F.P.S. Machado and J.A. Pamphile
This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.11
- DOI:
- 10.4238/2013.September.27.11
Construction of an integrated genetic map for Capsicum baccatum L.
Authors: M.M. Moulin, R. Rodrigues, H.C.C. Ramos, C.S. Bento, C.P. Sudr�?©, L.S.A. Gon�?§alves and A.P. Viana
Capsicum baccatum L. is one of the five Capsicum domesticated species and has multiple uses in the food, pharmaceutical and cosmetic industries. This species is also a valuable source of genes for chili pepper breeding, especially genes for disease resistance and fruit quality. However, knowledge of the genetic structure of C. baccatum is limited. A refer.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.12
- DOI:
- 10.4238/2015.June.18.12
Effect of two single nucleotide polymorphisms on milk yield and composition
Authors: J.C. Rinc�?³n, A. L�?³pez-Herrera and J.J. Echeverri
The bovine prolactin (PRL) and bovine growth hormone (bGH) genes exhibit several polymorphisms. Some of them can be detected by molecular techniques using restriction endonucleases, such as RsaI for the PRL gene and MspI for the bGH gene. We examined the relationship between the PRL-RsaI and bGH-MspI polymorphisms and some economically important character.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.15
- DOI:
- 10.4238/2013.April.2.15
Genetic diversity analysis of the Uruguayan Creole cattle breed using microsatellites and mtDNA markers
Authors: E. Armstrong, A. Iriarte, A.M. Mart�?nez, M. Feijoo, J.L. Vega-Pla, J.V. Delgado and A. Postiglioni
The Uruguayan Creole cattle population (N = 600) is located in a native habitat in south-east Uruguay. We analyzed its genetic diversity and compared it to other populations of American Creole cattle. A random sample of 64 animals was genotyped for a set of 17 microsatellite loci, and the D-loop hyper-variable region of mtDNA was sequenced for 28 calves o.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.7
- DOI:
- 10.4238/2013.April.10.7
Evaluation of genetic diversity in fig accessions by using microsatellite markers
Authors: A.D.B. do Val, C.S. Souza, E.A. Ferreira, S.M.L. Salgado, M. Pasqual and G.M.A. Can�?§ado
Fig (Ficus carica L.) is a fruit of great importance worldwide. Its propagation is carried out with stem cuttings, a procedure that favors the occurrence of synonymy among specimens. Thus, molecular markers have become an important tool for studies of DNA fingerprinting, germplasm characterization, and genetic diversity evaluation in this plant species. T.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.9
- DOI:
- 10.4238/2013.April.25.9
SSR-based association mapping of salt tolerance in cotton (Gossypium hirsutum L.)
Authors: Y.L. Zhao, H.M. Wang, B.X. Shao, W. Chen, Z.J. Guo, H.Y. Gong,X.H. Sang, J.J. Wang and W.W. Ye
The identification of simple sequence repeat (SSR) markers associated with salt tolerance in cotton contributes to molecular assisted selection (MAS), which can improve the efficiency of traditional breeding. In this study, 134 samples of upland cotton cultivars were selected. The seedling emergence rates were tested under 0.3.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027370
- DOI:
- 10.4238/gmr.15027370
Genetic variation in Rhodomyrtus tomentosa (Kemunting) populations from Malaysia as revealed by inter-simple sequence repeat markers
Authors: T.S. Hue, T.L. Abdullah, N.A.P. Abdullah and U.R. Sinniah
Kemunting (Rhodomyrtus tomentosa) from the Myrtaceae family, is native to Malaysia. It is widely used in traditional medicine to treat various illnesses and possesses significant antibacterial properties. In addition, it has great potential as ornamental in landscape design. Genetic variability studies are important for the rational management and conserv.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.10
- DOI:
- 10.4238/2015.December.14.10
Selfing rate estimation in sugarcane under unfavorable natural conditions of crossing by using microsatellite markers
Authors: M.L.G. Melloni , M.S. Scarpari, L.R. Pinto, D. Perecin, M.A. Xavier and M.G.A. Landell
The self-fertilization or selfing rate estimation using microsatellite markers and its impact on survival and selection rate were evaluated in families derived from polycrosses that involved parents that were widely used in sugarcane breeding in Brazil. These factors were evaluated under unfavorable natural conditions of flowe.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.8
- DOI:
- 10.4238/2014.March.31.8