Authors: W.J. Yuan, Y. Li, Y.F. Ma, Y.J. Han and F.D. Shang
Osmanthus fragrans (Oleaceae) is an evergreen shrub or small tree that grows in south China. In this study, Roche 454 FLX+ sequencing combined with the magnetic bead enrichment method was used to isolate microsatellite markers from the genome of O. fragrans. A total of 1471 microsatellites that contained enough flanking sequences for primer pair design we.. Read More»
Authors: X.Y. Du, Q.N. Hu, Q.L. Zhang, Y.B. Wang and Z.R. Luo
Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-c.. Read More»
Authors: T.J. Domingos, L.N. Moraes, R.M. Moresco, V.P. Margarido and P.C. Venere
Molecular genetic assessments that consider ecological information, in addition to endogamy levels, genetic diversity, and the genetic differentiation among species and populations, are particularly important for the conservation of biological diversity. Prime candidates for conservation genetic review are those subject to hum.. Read More»
Authors: J.J. Jin, H.Q. Wang, H.P. Kuang, B.B. Kang, Y.H. Liu and J. Wang
Lung cancer is the most common cancer occurring worldwide. The human X-ray repair complementing group 1 (XRCC1) gene is one of the most important candidate genes that influence the susceptibility to lung cancer. The objective of this study was to analyze the potential association between the c.1804C>A genetic variant of XRC.. Read More»
Authors: A.L. Ndoye, O. Sadio and D. Diouf
The genetic variation of 70 individual samples of Casuarina equisetifolia (L. Johnson) subsp equisetifolia and C. equisetifolia subsp incana growing along the northern coast of Senegal was analyzed with RAPD markers. Of the 160 primers tested, five were chosen; they generated 1396 reproducible bands and 61 polymorphic bands th.. Read More»
Authors: M. Balestre, R.G. Von Pinho and J.C. Souza
We examined the usefulness of the best linear unbiased prediction associated with molecular markers for prediction of untested maize double-cross hybrids. Ten single-cross hybrids from different commercial backgrounds were crossed using a complete diallel design. These 10 single-cross hybrids were genotyped with 20 microsatell.. Read More»
Authors: M. Elangbam and A.K. Misra
Tea leaves are rich in plant secondary phenolics, especially flavonoids. Catechins are considered to be the most valuable flavonoids, and the catechin content in tea is an important trait for determining its quality. We have developed cleaved amplified polymorphic sequence (CAPS)-based markers for evaluating total catechin con.. Read More»
Authors: Z.D. Zhao, A.N. Li, S.J. Wei, M.M. Wang, S.J. Li and L.S. Zan
Fatty acid transport protein 1 (FATP1), an integral membrane protein that facilitates long-chain fatty acid influx, is involved in the genetic network for oleic acid synthesis. The aim of this study was to examine the association of FATP1 polymorphisms with live animal meat quality traits in Chinese Qinchuan cattle. Quantitative real-time PCR analysis dem.. Read More»
Authors: S. Ghosh, P.B. Majumder and S. Sen Mandi
The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most imp.. Read More»
Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang
Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences.. Read More»