Molecular Marker
Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery
Authors: L.Y. Yu, J.J. Bai, J.J. Fan, D.M. Ma, Y.C. Quan and P. Jiang
Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.35
- DOI:
- 10.4238/2015.December.29.35
A priori choice of hybrid parents in plants
Authors: Luiz Ant�´nio dos Santos Dias, Edgard Augusto de Toledo Picoli, Rodrigo Barros Rocha and Acelino Couto Alfenas
Plant breeding deals with high-yielding genotypes. However, how best to choose parents of these genotypes remains an unsolved question. Here, we focus on a priori choice based on parental distances by means of agronomic and molecular data. Despite numerous theoretical and empirical studies, a priori choice continues to be a co.. Read More»
- Genet. Mol. Res. 3(3):