Molecular Marker
A RAPD-PCR-based genetic diversity analysis of Helicoverpa armigera and H. zea populations in Brazil
Authors: H.M. Lopes, C.S. Bastos, L.S. Boiteux, J. Foresti and F.A. Suinaga
Helicoverpa armigera is the most significant pest of agriculture in Asia, Europe, Africa, and Australasia, causing damage to crops greater than US$2 billion annually and until 2013 it was not detected in Brazil. Helicoverpa zea is restricted to the American continent and is important to corn and a secondary pest of cotton and .. Read More»
- Genet. Mol. Res. 16(3):
- gmr16038757
- DOI:
- 10.4238/gmr16038757
Analysis of genetic variability of commercial melon cultivars using SSR molecular markers
Authors: N. Carvalho, F.M. Canela, P.H.S. Leite, M.A. Ferreira, V.R. Oliveira, M.F. Santos, N.O.S. Souza and G.S.C. Buso
Taking into account the scenario of melon production (Cucumis melo L.) in Brazil, it is notable the importance of the culture in the fruit market. The preference of the national market is concentrated in the fruits of the yellow type. Therefore, it is possible that the genetic base of the commercial cultivars is relatively nar.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039739
- DOI:
- 10.4238/gmr16039739
Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials
Authors: R.M.O. Pires, M.A.B. Ã?Â?vila, D.G. Leite, H.O. Santos, G.A. Souza and E.V.R. Von Pinho
With the imminence of global climate changes that affect the temperature and the rainfall uniformity, it is growing the concern about the adaptation of crops to the water deficit. Thus, the objective of this study was to evaluate alterations in physiological and enzymatic mechanisms during the germination process of sesame see.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039425
- DOI:
- 10.4238/gmr16039425
An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae)
Authors: S.R. Arruda, D.G. Pereira, M.M. Silva-Castro, M.G. Brito and A.M. Waldschmidt
Some species are characterized by a high content of tannins, alkaloids, and phenols in their leaves. These secondary metabolites are released during DNA extraction and might hinder molecular studies based on PCR (polymerase chain reaction). To provide an efficient method to extract DNA, Mimosa tenuiflora, an important legumino.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039063
- DOI:
- 10.4238/gmr16039063
Identification of Colletotrichum isolates from Capsicum chinense in Amazon
Authors: C.V.S. de Oliveira, K.S. Matos, D.M.C. de Albuquerque, R.E. Hanada and G.F. da Silva
Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colle.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029601
- DOI:
- 10.4238/gmr16029601
Assessment of allelic diversity among drought-resistant cotton genotypes using microsatellite markers
Authors: A. Javaid, F.S. Awan, F.M. Azhar and I.A. Khan
Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (S.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029664
- DOI:
- 10.4238/gmr16029664
High genetic diversity of Jatropha curcas assessed by ISSR
Authors: B.G. DÃ?Âaz, D.M. Argollo, M.C. Franco, S.M. Nucci, W.J. Siqueira, D.M. de Laat and C.A. Colombo
Jatropha curcas L. is a highly promising oilseed for sustainable production of biofuels and bio-kerosene due to its high oil content and excellent quality. However, it is a perennial and incipiently domesticated species with none stable cultivar created until now despite genetic breeding programs in progress in several countri.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029683
- DOI:
- 10.4238/gmr16029683
Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits
Authors: J.C. Costa, G.G.M. Fracetto, F.J.C. Fracetto, M.V.F. Santos and M.A. Lira Junior
Desmanthus is a genus of forage legumes with high nutritional value, productive potential, and ability to obtain nitrogen in association with diazotrophic bacteria. The use of accurate techniques for genotype identification and characterization is essential for breeding programs. Morphological markers are widely used to know t.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029667
- DOI:
- 10.4238/gmr16029667
Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down stream processes
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu, N. Na and Z. Huiyan
We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from etiolated leaves of wheat crop (Triticum aestivum). mtDNA extraction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applicati.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.27
- DOI:
- 10.4238/2014.December.4.27
Polymorphic microsatellite markers for the rare and endangered cactus Uebelmannia pectinifera (Cactaceae) and its congeneric species
Authors: E.M. Moraes1, F.W. Cidade1, G.A.R. Silva1 and M.C. Machado2
The cactus genus Uebelmannia includes 3 narrow endemic species associated with rocky savanna habitats in eastern South America. Because of their rarity and illegal over-collection, all of these species are endangered. Taxonomic uncertainties resulting from dramatic local variation in morphology within Uebelmannia species preclude effective conservation ef.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.31
- DOI:
- 10.4238/2014.December.4.31