Molecular Marker
Genetic-molecular characterization of backcross generations for sexual conversion in papaya (Carica papaya L.)
Authors: H.C.C. Ramos1, M.G. Pereira1, T.N.S. Pereira1, G.B.A. Barros1 and G.A. Ferreguetti2
The low number of improved cultivars limits the expansion of the papaya crop, particularly because of the time required for the development of new varieties using classical procedures. Molecular techniques associated with conventional procedures accelerate this process and allow targeted improvements. Thus, we used microsatellite markers to perform geneti.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.32
- DOI:
- 10.4238/2014.December.4.32
Development of 1047 insertion-deletion markers for rice genetic studies and breeding
Authors: Y.X. Zeng*, Z.H. Wen*, L.Y. Ma, Z.J. Ji, X.M. Li and C.D. Yang
In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and j.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.7
- DOI:
- 10.4238/2013.October.30.7
Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle
Authors: D.B.S. Silva, B.A. Crispim, L.E. Silva, J.A. Oliveira, F. Siqueira, L.O. Seno and A.B. Grisolia
The aim of the study was to detect polymorphisms in the leptin gene and to determine the association of these polymorphisms with growth and carcass traits in Nellore cattle. The single nucleotide polymorphisms (SNPs) -1457 (AJ571671:g.-1457A>G) and A59V (AF536174.1:g. 321C>T), as well as the microsatellite BM1500 (3.9 kb.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.9
- DOI:
- 10.4238/2014.April.16.9
Isolation and characterization of polymorphic microsatellite loci in the swimming crab Portunus trituberculatus (Portunidae) L.P.
Authors: L.P. Ren, Y. Qin, X.C. Li, Y.N. Sun, R.X. Wang
The Swimming crab Portunus trituberculatus (Portunidae) is an important economically food species. To provide molecular markers for P. trituberculatus, we isolated and characterized polymorphic microsatellite markers. We developed a 5'-anchored genomic library of P. trituberculatus DNA, and derived 45 positive clones. We desig.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.19
- DOI:
- 10.4238/2013.November.22.19
Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species
Authors: Y.T. Liu, R.K. Chen, S.J. Lin, Y.C. Chen, S.W. Chin, F.C. Chen and C.Y. Lee
The Orchidaceae is one of the largest and most diverse families of flowering plants. The Dendrobium genus has high economic potential as ornamental plants and for medicinal purposes. In addition, the species of this genus are able to produce large crops. However, many Dendrobium varieties are very similar in outward appearance.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.15
- DOI:
- 10.4238/2014.April.8.15
Isolation and characterization of microsatellites for the yam Dioscorea cayenensis (Dioscoreaceae) and cross-amplification in D. rotundata
Authors: L.R.G. Silva, M.M. Bajay, M. Monteiro, T.F. Mezette, W.F. Nascimento, M.I. Zucchi, J.B. Pinheiro and E.A. Veasey
Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for trans.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.5
- DOI:
- 10.4238/2014.April.14.5
Population variability of Bemisia tabaci (Genn.) in different hosts
Authors: G.E. do Valle, A.L. LourenĂ?§Ă?£o, M.I. Zucchi, J.B. Pinheiro and A.G. de Abreu
The silverleaf whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is a cryptic species complex that contains some of the most damaging pests in tropical and subtropical regions. Recent studies have indicated that this complex is composed of at least 24 distinct and morphologically indistinguishable species that mainly differ in their ability to tr.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.17.4
- DOI:
- 10.4238/2013.October.17.4
Analysis of mitochondrial DNA using amplified fragment length polymorphism markers of isonuclear allocytoplasmic male sterile wheat accessions and their maintainer lines
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu and Z. Xinbo
To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta (T.spelta) -9.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.5
- DOI:
- 10.4238/2013.October.30.5
Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting
Authors: H.S. An, J.Y. Park, J.I. Myeong and C.M. An
Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and H. diversicolor diversicolor, whose morphologic.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.18
- DOI:
- 10.4238/2013.December.4.18
Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)
Authors: J.Y. Zheng, H. Wang, X.X. Chen, P. Wang, P. Gao, X.N. Li and G.P. Zhu
Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellit.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.1
- DOI:
- 10.4238/2012.August.6.1