Molecular Marker
Genetic diversity and structure of natural Dactylis glomerata L. populations revealed by morphological and microsatellite-based (SSR/ISSR) markers
Authors: P. Madesis1, E.M. Abraham2, A. Kalivas, Ã?Â?. Ganopoulos and A. Tsaftaris
Dactylis glomerata L. is an important forage species in the Mediterranean region, and in other regions with a similar climate. Genetic material from 3 locations in north, central, and south Greece was studied, using morphological traits, SSR, and ISSR molecular markers. Morphological analysis revealed differences among the geo.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.8
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.8
Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa
Authors: W.J. Li, H. Ma, Z.H. Li, Y.M. Wan, X.X. Liu and C.L. Zhou
We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism... Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.13
- DOI:
- 10.4238/2012.August.6.13
A new strategy employed for identification of sweet orange cultivars with RAPD markers
Authors: X. Sun, Q. Mu, D. Jiang , C. Wang, X.C. Wang and J.G. Fang
We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.11
- DOI:
- 10.4238/2012.August.6.11
Using SCC8, SCF27 and VMC7f2 markers in grapevine breeding for seedlessness via marker assisted selection
Authors: M. Akkurt, A. ��ak�±r, M. Shidfar, B.P. ��elikkol and G. S�¶ylemezo��lu
We used molecular markers associated with seedlessness in grapes, namely SCC8, SCF27 and VMC7f2, to improve the efficiency of seedless grapevine breeding via marker assisted selection (MAS). DNA from 372 F1 hybrid progeny from the cross between seeded “Alphonse Lavallée” and seedless “Sultani” was amplified by PCR using thre.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.2
- DOI:
- 10.4238/2012.August.13.2
Verification and evaluation of grain QTLs using RILs from TD70 x Kasalath in rice
Authors: Y.D. Zhang, J. Zheng, Z.K. Liang, Y.L. Liang, Z.H. Peng and C.L. Wang
Grain size is an important trait that directly influences the yield of rice. Validation and evaluation of grain genes is important in rice genetic studies and for breeding. In a population of 240 recombinant inbred lines (RILs) derived from a cross between an extra-large grain japonica variety TD70 and a small grain .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.53
- DOI:
- 10.4238/2015.November.18.53
Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers
Authors: Y.S. Lin, C.S. Kuan, I.S. Weng and C.C. Tsai
The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.11
- DOI:
- 10.4238/2015.November.24.11
Genetic structure and diversity analysis of the primary gene pool of chickpea using SSR markers
Authors: P. Choudhary, S.M. Khanna, P.K. Jain, C. Bharadwaj, J. Kumar, P.C. Lakhera and R. Srinivasan
Members of the primary gene pool of the chickpea, including 38 accessions of Cicer arietinum, six of C. reticulatum and four of C. echinospermum grown in India were investigated using 100 SSR markers to analyze their genetic structure, diversity and relationships. We found considerable diversity, with a mean of 4.8 alleles per.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.5
- DOI:
- 10.4238/2012.April.10.5
Genetic variability in local Brazilian horse lines using microsatellite markers
Authors: A.C.M. Silva, S.R. Paiva, M.S.M. Albuquerque, A.A. Egito, S.A. Santos, F.C. Lima, S.T. Castro, A.S. Mariante, P.S. Correa and C.M. McManus
Genetic variability at 11 microsatellite markers was analyzed in five naturalized/local Brazilian horse breeds or genetic groups. Blood samples were collected from 328 animals of the breeds Campeira (Santa Catarina State), Lavradeira (Roraima State), Pantaneira (Pantanal Mato-Grossense), Mangalarga Marchador (Minas Gerais Stat.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.4
- DOI:
- 10.4238/2012.April.10.4
Genetic diversity of cultivated and wild tomatoes revealed by morphological traits and SSR markers
Authors: R. Zhou, Z. Wu, X. Cao and F.L. Jiang
In the current study, morphological traits and molecular markers were used to assess the genetic diversity of 29 cultivated tomatoes, 14 wild tomatoes and seven introgression lines. The three components of the principal component analysis (PCA) explained 78.54% of the total morphological variation in the 50 tomato genotypes assessed. Based on these morpho.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.7
- DOI:
- 10.4238/2015.October.29.7
Natural hybridization between Gossypium mustelinum and exotic allotetraploid cotton species
Authors: I.P.P. de Menezes1, J.O. da Silva1, G. Malafaia1, R.D.D. Silveira1 andP.A.V. Barroso2
Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridiza.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.40
- DOI:
- 10.4238/2015.October.29.40