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Molecular Marker

Plant Genetics   Research Article

An improved strategy based on RAPD markers efficiently identified 95 peach cultivars

Authors: M.L. Yu, W.Y. Wang, R.J. Ma, Z.J. Shen and J.G. Fang

DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11.. Read More»

Genet. Mol. Res. 11(2):
2012.May.7.1
DOI:
10.4238/2012.May.7.1
Microbial Genetics   Research Article

Breeding of a target genotype variety based on identified chalkiness marker-QTL associations in rice (Oryza sativa L.)

Authors: X. Liu, Y.R. Du, X.H. Li, X.L. Li, W.Q. Yang and Y. Wang

The aim of this study was to breed a target genotype variety based on the identified chalkiness marker-QTL (quantitative trait locus) associations in rice. First, a permanent mapping population of rice that consisted of 525 recombinant inbred lines (RILs), which were derived from Zhenshan 97/Minghui 63, was used to identify QTLs with additive effects for .. Read More»

Genet. Mol. Res. 14(4):
2015.October.21.10
DOI:
10.4238/2015.October.21.10
Cytogenomics   Research Article

New microsatellite markers for the abalone Haliotis midae developed by 454 pyrosequencing and in silico analyses

Authors: R. Slabbert J.-A. Hepple C. Rhode A.E. Bester-Van der Merwe R. Roodt-Wilding

Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enrich.. Read More»

Genet. Mol. Res. 11(3):
2012.August.24.2
DOI:
10.4238/2012.August.24.2
Plant Genetics   Research Article

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Authors: P.R. Da-Silva S.P. Brammer D. Guerra S.C.K. Milach A.L. Barcellos M.I. Baggio

Leaf rust is one of the most destructive diseases affecting wheat worldwide. The most effective way to control it is to use resistant cultivars. Resistance based on slow-rusting adult plant resistance (APR) genes has proven to be the best method for developing cultivars with durable resistance. A source of slow-rusting APR for leaf rust is the Brazilian whea.. Read More»

Genet. Mol. Res. 11(3):
2012.August.24.7
DOI:
10.4238/2012.August.24.7
Plant Genetics   Research Article

Molecular characterization of wheat germplasm using microsatellite markers

Authors: S. Ijaz and I.A. Khan

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into di.. Read More»

Genet. Mol. Res. 8(3):
Plant Genetics   Research Article

Genetic distances between popcorn populations based on molecular markers and correlations with heterosis estimates made by diallel analysis of hybrids

Authors: R.E.F. Munhoz1, A.J. Prioli1, A.T. Amaral J�ºnior2, C.A. Scapim1 and G.A. Simon1

Diallel analysis was used to obtain information on com­bining ability, heterosis, estimates of genetic distances by random amplified polymorphic DNA (RAPD) and on their correlations with heterosis, for the popcorn varieties RS 20, UNB2, CMS 43, CMS 42, Zélia, UEM J1, UEM M2, Beija-Flor, and Viçosa, which were crossed to obtain all possib.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr592
DOI:
10.4238/vol8-3gmr592
Plant Genetics   Research Article

Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

Authors: P. Khajudparn T. Prajongjai O. Poolsawat P.A. Tantasawat

Mungbean improvement via hybridization requires the identification of true F1 hybrids from controlled crosses before further generations of selfing/crossing and selection. We utilized inter-simple sequence repeat (ISSR) markers for identifying putative F1 hybrids from six cross combinations whose morphological characteristics were very similar to those of th.. Read More»

Genet. Mol. Res. 11(3):
2012.September.17.3
DOI:
10.4238/2012.September.17.3
Bioinformatics   Research Article

Identification of earl millet cultivars using both microsatellites and enzymatic markers

Authors: R.P. Mendon�§a Neto E.V.R. Von Pinho B.L. Carvalho G.S. Pereira

The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin,.. Read More»

Genet. Mol. Res. 12(1):
2013.January.7.1
DOI:
10.4238/2013.January.7.1
Microbial Genetics   Research Article

Genetic divergence among Brazilian garlic cultivars based on morphological characters and AFLP markers

Authors: R.G.F. Morales J.T.V. Resende F.V. Resende C.A. Delatorre A.S.T. Figueiredo P.R. Da-Silva

Outside its centers of origin, garlic propagates only asexually. Since asexual reproduction leads to the absence of meiotic recombination, the main garlic cultivars available for cultivation have arisen from the accumulation of somatic mutations in early cultivars. Thus, it is common for a single clone to have different names in different regions. This st.. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.1
DOI:
10.4238/2013.February.4.1
Animal Genetics   Research Article

Microsatellite markers for genetic studies of the fall armyworm, Spodoptera frugiperda

Authors: V.A.C. Pavinato S. Martinelli P.F. de Lima M.I. Zucchi C. Omoto

We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrants excha.. Read More»

Genet. Mol. Res. 12(1):
2013.February.8.1
DOI:
10.4238/2013.February.8.1