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Mrna

Microbial Genetics   Research Article

Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara

Authors: Z.W. Zhang X.F. Shi M. Qu S.X. Ding

Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted gro.. Read More»

Genet. Mol. Res. 11(3):
2012.May.11.1
DOI:
10.4238/2012.May.11.1
Animal Genetics   Research Article

Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin, serum concentration of amino acids, and growth performance of pigs

Authors: M. Cervantes-Ram�­rez V. Mendez-Trujillo B.A. Araiza-Pi�±a M.A. Barrera-Silva D. Gonz�¡lez-Mendoza A. Morales-Trejo

Leucine (Leu) participates in the activity of cationic amino acid (aa) transporters. Also, branched-chain aa [Leu, isoleucine (Ile), and valine (Val)] share intestinal transporters for absorption. We conducted an experiment with 16 young pigs (body weight of about 16 kg) to determine whether Leu and Ile affect expression of aa transporters b0,+ and CAT-1 .. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.3
DOI:
10.4238/2013.January.24.3
Microbial Genetics   Research Article

Effects of exogenous protease supplementation on endogenous trypsin activity and gene expression in broilers

Authors: L. Yuan, S.Q. Wang, Z.X. Wang, H. Zhu and K. Huang

Two hundred and forty one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets with 0, 40, 80, or 160 mg/kg of an acid protease preparation from 1 to 42 days of age, in order to investigate the effects of an exogenous enzyme on growth performance, pancreatic trypsin activity and mRNA expression. Average daily gain (ADG) was greatest .. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.25
DOI:
10.4238/2015.October.28.25
Animal Genetics   Research Article

Differential expression of FUT1 and FUT2 in Large White, Meishan, and Sutai porcine breeds

Authors: R.W. Xia1, W.Y. Qin1, L.N. Gan1, J. Wang1, S.L. Wu1,2 and W.B. Bao1,2

To assess the relationship between the expression of a(1,2)-fucosyltransferase (FUT1 and FUT2) genes and resistance to Escherichia coli F18 in weaned pigs, FUT1 and FUT2 expression levels in Large White, Meishan, and Sutai pigs (with resistance to E. coli F18) were determined using real-time PCR. The results revealed that FUT1 and FUT2 expression levels w.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017613
DOI:
10.4238/gmr.15017613
Animal Genetics   Research Article

Molecular characterization, expression, and functional analysis of chicken TRAF6

Authors: J. Jin, J.S. Ran, C.W. Yang, X.S. Jiang, Y.G. Zhou, Z.Q. Feng, Y. Wang, D. Lan, P. Ren and Y.P. Liu

Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial adaptor molecule of the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily, which can trigger downstream signaling cascades involved in innate immunity. The function of TRAF6 has been clarified in mammals but is poorly understood in chicken. .. Read More»

Genet. Mol. Res. 16(1):
gmr16019138
DOI:
10.4238/gmr16019138
Human Genetics   Research Article

Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases

Authors: J. Luo, K. Yang and Y.G. Wen

The incidence of liver cancer has gradually risen to a high level in China, and tumor metastasis occurs via multiple pathways. Alpha fetal protein (AFP) is the main biomarker of liver cancer micrometastases. A recent study showed that glypican-3 (GPC3), which is abundant in hepatoma cells, has promising specificity and could b.. Read More»

Genet. Mol. Res. 16(1):
gmr16018947
DOI:
10.4238/gmr16018947
Plant Genetics   Short commentary

Differential expression analysis of porcine MDH1, MDH2 and ME1 genes in adipose tissues

Authors: S.L. Zhou, M.Z. Li, Q.H. Li, J.Q. Guan and X.W. Li

Malate dehydrogenases 1 and 2 (MDH1 and MDH2), and malic enzyme 1 (ME1) play important roles in the Krebs cycle for energy metabolism. The mRNA abundance changes of MDH1, MDH2 and ME1 genes were measured across six different adipose tissues from the leaner Landrace and fatty Rongchang pig breeds using quantitative real-time PC.. Read More»

Genet. Mol. Res. 11(2):
2012.May.9.4
DOI:
10.4238/2012.May.9.4
Animal Genetics   Research Article

Differential expression of peroxisome proliferator-activated receptor �³, fatty acid synthase, and hormone-sensitive lipase in fat-tailed and thin-tailed sheep breeds

Authors: X.C. Xu, B.B. Li, X. Wei, Y.X. Yang, X.L. Wang, Y.L. Chen

Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan shee.. Read More»

Genet. Mol. Res. 14(4):
Animal Genetics   Research Article

Differential expression of peroxisome proliferator-activated receptor �³, fatty acid synthase, and hormone-sensitive lipase in fat-tailed and thin-tailed sheep breeds

Authors: X.C. Xu,B.B. Li,X. Wei,Y.X. Yang,X.L. Wang,Y.L. Chen

Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan shee.. Read More»

Genet. Mol. Res. 14(4):
2015.December.1.14
DOI:
10.4238/2015.December.1.14
Human Genetics   Research Article

Matrix metalloproteinase-3 gene polymorphism and its mRNA expression in rheumatoid arthritis

Authors: M.J. Ma, H.C. Liu, X.Q. Qu, J.L. Wang

Matrix metalloproteinase-3 (MMP-3) can mediate the occurrence and development of rheumatoid arthritis (RA). The MMP3 promoter gene exhibits polymorphism with 5A/6A alleles. We investigated the correlation between the expression of MMP3 gene polymorphism and RA to provide an objective basis for prognosis evaluation. We enrolled.. Read More»

Genet. Mol. Res. 14(4):
2015.December.1.17
DOI:
10.4238/2015.December.1.17