Polymerase Chain Reaction
Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species
Authors: Asaad Gaber, Hzem Hamed, Essam Elsawy
Low-cost and low-density (LCD) DNA arrays offer an easy way to detect resistance as minimal laboratory instrumentation is needed. Nucleic acid-based amplification tests allow the rapid detection of Mycobacterium tuberculosis. Recently, a real-time PCR assay for M. tuberculosis complex was introduced. Real-Time PCR and DNA-micr.. Read More»
- Genet. Mol. Res. 16(4):
- gmr16039837
- DOI:
- 10.4238/gmr16039837
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa
Authors: V.M. Almeida, G.A. Luz, P.P. Martins, M.F.C. Gomes, M.F. Costa, P.S.C. Lima and S.E.S. Valente
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, ma.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039724
- DOI:
- 10.4238/gmr16039724
Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain
Authors: J.H. Hu1,2*, F.Y. Zhang1*, K.J. Jiang1, Y.B. Fang1,2, J. Wang1,2, M. Zhao1,2, Z.G. Qiao1 and L.B. Ma1
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.19
- DOI:
- 10.4238/2014.December.4.19
Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance
Authors: J. Wang, Y. Liu, W.H. Dong, Y.J. Huo, X.G. Huang, S.L. Wu and W.B. Bao
The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expressi.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.12
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.12
Expression analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle
Authors: Y. Liu, H. Wang, G. Cheng B. Jiang and L. Zan
The Hedgehog (Hh) signaling pathway regulates the differentiation of many kinds of cells and plays a critical role in many embryonic and postnatal developmental processes. Gli1 and Gli2 are two transcription factors of the Hh signaling pathway. In this study, we used quantitative real-time polymerase chain reaction to detect the relative expression of Gli.. Read More»
- Genet. Mol. Res. 13(4):
Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori
Authors: Q.M. Chen, D.J. Cheng, S.P. Liu, Z.G. Ma, X. Tan and P. Zhao
Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven gene.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.13.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.13.2
Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil
Authors: J. Coser, T. da Rocha Boeira, D. Simon, A.S. Kazantzi Fonseca, N. Ikuta and V.R. Lunge
Human papillomavirus (HPV) infection is a common viral sexually transmitted infection and the main cause of cervical cancer in women worldwide. Epidemiological data on the prevalence of HPV in a given population is essential for the establishment of effective prevention strategies. The aim of this study was to determine HPV prevalence in women who attende.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.19.3
- DOI:
- 10.4238/2013.February.19.3
Expression of immune response genes in peripheral blood of cattle infested with Rhipicephalus microplus
Authors: R. Domingues, S. Wohlres-Viana, D.R.L. Reis, H.C. Teixeira, A.P. Ferreira, S.E.F. Guimar�£es, M.C.A. Prata, J. Furlong, R.S. Verneque and M.A. Machado
The bovine tick Rhipicephalus microplus is responsible for severe economic losses in tropical cattle production. Bos indicus breeds are more resistant to tick infestations than are Bos taurus breeds, and the understanding of the physiological mechanisms involved in this difference is important for the development of new method.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.12
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.12
Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear
Authors: J. Tang, J. Lin, B.L. Zhang, Z.H. Wang, X.G. Li and Y.H. Chang
A 1794-bp cDNA fragment was amplified from mRNA isolated from pear (Pyrus pyrifolia NaKai. Cuiguan) leaves by using primers based on the sequences generated during the analysis of the pear transcriptome. The 597-amino acid sequence encoded by the cDNA was compared with the sequences in GenBank, and it was found to be similar to that of members of the sucr.. Read More»
- Genet. Mol. Res. 13(4):
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
Authors: F.J. Bello, N.A. Segura and M. Ruiz-Garcia
Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito’s genetic variability and genetic structure of 122 spec.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.6