Polymerase Chain Reaction
Simultaneous selection for cowpea (Vigna unguiculata L.) genotypes with adaptability and yield stability using mixed models
Authors: F.E. Torres, P.E. Teodoro, E.V. Rodrigues, A. Santos, A.M. Corr?ªa, G. Ceccon
The aim of this study was to select erect cowpea (Vigna unguiculata L.) genotypes simultaneously for high adaptability, stability, and yield grain in Mato Grosso do Sul, Brazil using mixed models. We conducted six trials of different cowpea genotypes in 2005 and 2006 in Aquidauana, Chapadão do Sul, Dourados, and Primave.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028272
- DOI:
- 10.4238/gmr.15028272
Biological correlation between glucose transporters, Ki-67 and 2-deoxy-2-[18F]-fluoro-D-glucose uptake in diffuse large B-cell lymphoma and natural killer/T-cell lymphoma
Authors: Y.M. Liu, X.M. Zhai and Y.W. Wu
The purpose of this study was to investigate the association between cellular 2-deoxy-2-[18F]-fluoro-D-glucose (18F-FDG) uptake and the expression of several subtypes of glucose transporters (GLUT) and Ki-67 in diffuse large B-cell lymphoma (DLBCL) and natural killer (NK)/T-cell lymphoma (NKTCL). Cell lines were histologically.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027242
- DOI:
- 10.4238/gmr.15027242
Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid nitrogen
Authors: C.Y. Michel-L?³pez, D. Gonz?¡lez-Mendoza and O. Grimaldo-Juarez
The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of li.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.10
- DOI:
- 10.4238/2013.September.27.10
Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples
Authors: X. Liu, Y.Y. Yang, X.M. Wang, Z.S. Liu, Z.H. Wang and Y.Z. Ding
The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this metho.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.13
- DOI:
- 10.4238/2015.August.7.13
Decreased microRNA-143 expression and its tumor suppressive function in human oral squamous cell carcinoma
Authors: Z.Y. Ni, F.O. Lin, D.F. Liu and J. Xiao
MicroRNA-143 serves as a tumor suppressor in many human malignancies. However, its involvement in oral squamous cell carcinoma (OSCC) is still unclear. In this study, we investigated the effects of miR-143 in OSCC tumorigenesis and development. Using real-time quantitative reverse transcription-polymerase chain reaction, we detected miR-143 expression in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.2
- DOI:
- 10.4238/2015.June.26.2
Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco
Authors: A.M. Ding, F.X. Li, Y.Q. Chen,, P. Zong, X. Qu, D.P. Gong, G.S. Liu and Y.H. Sun
As a model plant, mechanisms of the cytoplasmic male sterility/restoration of fertility (CMS/Rf) system in tobacco are seldom studied. Using Rf gene sequences from other Solanaceae plants and the draft genome of Nicotiana benthamiana, degenerate primers were designed to amplify the cDNA pool of N. tomentosiformis. In total, si.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.11
- DOI:
- 10.4238/2014.March.31.11
Differential gene expression in drought-tolerant sugarcane roots
Authors: J.S. Vantini, G.C. Dedemo, D.F. Jovino Gimenez, L.F.S. Fonseca, R.I.D. Tezza, M.A. Mutton, J.A. Ferro and M.I.T. Ferro
Drought is one of the most frequent abiotic stresses limiting the productivity and geographical distribution of sugarcane culture. The use of drought-tolerant genotypes is one approach for overcoming the effects of water stress. We conducted a comparative study to identify gene expression profiles under water stress in tolerant sugarcane roots. Two differ.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.13
- DOI:
- 10.4238/2015.June.29.13
Operational taxonomic units (OTUs) of endophytic bacteria isolated from banana cultivars in the Amazon
Authors: ?. Souza, V.B. Nogueira, J.C. Cruz, N.R. Sousa, A.R.L. Proc?³pio and G.F. Silva
Endophytic microorganisms colonize plants, inhibit the growth of pathogens (by competing for nutrients and/or space), or produce antagonistic substances. Fifty-five endophytic bacteria were isolated from the leaf tissue of the FHIA 18 banana cultivar. Genetic diversity analyses were performed using the enterobacterial repetitive intergenic consensus seque.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.28
- DOI:
- 10.4238/2015.August.19.28
HLA-A gene polymorphisms contribute to osteoporosis susceptibility in postmenopausal Han Chinese women
Authors: S.M. Li, H. Guo, H.J. Yang, M.Q. Lv and D.X. Zhou
Osteoporosis is a common disease characterized by low bone mineral density, deterioration in bone microarchitecture, and increased fracture risk and is more prevalent in postmenopausal women. HLA is a complex gene family; previous studies have shown that it plays an important role in the pathogenesis of osteoporosis among Japanese and Greek populations. P.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.18
- DOI:
- 10.4238/2015.August.28.18
Association of TNF-?± G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus
Authors: N. Ghodsian, M. Akhlaghi, V. Ramachandran, F. Heidari, P. Haghvirdizadeh, S.A. Eshkoor, A. Etemad, J.A. Jamaluddin and P. Ismail
This study aims to investigate the effects of tumor necrosis factor alpha (TNF-α) G308A gene polymorphism on essential hypertension (EHT) with or without type 2 diabetes mellitus (T2DM). The project was conducted on buccal epithelial and blood cells for case and control patients, respectively. Epithelial cells were obtained from the inner part of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.4
- DOI:
- 10.4238/2015.December.29.4