Polymerase Chain Reaction
Molecular cloning and cDNA characterization of Camelus dromedarius putative cytochrome P450s 1A, 2C, and 3A
Authors: H.M. Saeed, M.S. Alanazi, M.A. Shalaby, O. Alshahrani, F.S. Ataya, A.A. Pathan and Z.A Abduljaleel
The domesticated one-humped Arabian camel, Camelus dromedarius, is one of the most important animals in the Arabian Peninsula. Most of its life, this animal is exposed to both intrinsic and extrinsic genotoxic factors that are known to cause gross metabolic alterations in many organisms. This study determined the full length c.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.March.17.1
- DOI:
- 10.4238/2014.March.17.1
Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells
Authors: Q.D. Jiang, H.P. Li, F.J. Liu, X.J. Wang, Y.J. Guo, L.F. Wang, W.F. Lu, H.J. Li, X.P. Li and Y.Y. Wang
This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 106 cells per dish). The test was divided into 6 group.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.24.12
- DOI:
- 10.4238/2014.January.24.12
Molecular characterization of tumor necrosis factor receptor-associated factor 6 (TRAF6) in pearl oyster Pinctada martensii
Authors: Y. Jiao, Q.L. Tian, X.D. Du, Q.H. Wang, R.L. Huang, Y.W. Deng and S.L. Shi
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a key signaling adaptor molecule for tumor necrosis factor receptor superfamily and Toll-like receptor/interleukin-1 receptor family members. It signals the upstream receptors and is involved in a wide range of biological functions, such as immunity and bone metabolism. In this report, the TRAF.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.17
- DOI:
- 10.4238/2014.December.12.17
Cloning of flanking sequence in transgenic plants by restriction site-anchored single-primer polymerase chain reaction
Authors: J. Ma, N.N. Wang, S. Ren, Y.P. Fu, S. Lu, Y.P. Wang and P.W. Wang
Determining the insertion position of an exogenous gene in the target plant genome is one of the main issues in the transgenic plant field. This study introduced a simple, rapid, and accurate method to clone the flanking sequences of the transgenic bar gene as the anchoring gene in the transgenic maize genome using single-primer polymerase chain reaction .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.18
- DOI:
- 10.4238/2014.December.12.18
`Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients
Authors: C.L. Wei1, J.L. Cheng, W.C. Yang, L.Y. Li, H.C. Cheng and J.J. Fu1
Y chromosomal microdeletions at the azoospermia factor locus and chromosome abnormalities have been implicated as the major causes of idiopathic male infertility. A marker chromosome is a structurally abnormal chromosome in which no part can be identified by cytogenetics. In this study, to identify the origin of the marker chromosomes and to perform a gen.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.11
- DOI:
- 10.4238/2015.November.18.11
Leishmania (Viannia) braziliensis in dogs in Brazil: epidemiology, co-infection, and clinical aspects
Authors: F.S. Carvalho, A.A. Wenceslau, G.R. Albuquerque, A.D. Munhoz, E. Gross, P.L.S. Carneiro, H.C. Oliveira, J.M. Rocha, I.A. Santos and R.P. Rezende
Leishmaniasis is an endemic disease present in 98 countries. In Brazil, the northeast region accounts for approximately half of the cases in humans, and has experienced an increased number of positive cases in dogs. In this study, we investigated the epidemiology of canine leishmaniasis in the city of Ilhéus, Bahia, usi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.19
- DOI:
- 10.4238/2015.October.5.19
Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil
Authors: R. Strapazzon, F.E. Carneiro, J.C.V. Guerra Jr. and G. Moretto
The mite Varroa destructor is an ectoparasite that is considered a major pest for beekeeping with European honey bees. However, Africanized bee colonies are less threatened by this ectoparasite, because infestation levels remain low in these bees. The low reproductive ability of female mites of the Japanese biotype (J), introduced to Brazil early in .. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr567
- DOI:
- 10.4238/vol8-3gmr567
Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells
Authors: H.-Y. Liu M.-M. Yang Y.-H. Cui T. Ma X.-Q. Liu L. Bai W.-P. Xiao H.-B. Zhao J.-Y. Peng X.-B. Hu B.-Y. Cao
Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fl.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.4
- DOI:
- 10.4238/2013.January.24.4
Association of N-acetyltransferase-2 polymorphism with an increased risk of coronary heart disease in a Chinese population
Authors: J.D. Sun, H. Yuan, H.Q. Hu and H.M. Yu
We investigated the possible correlations between N-acetyltransferase-2 (NAT2) gene polymorphisms and the risk of coronary heart disease (CHD). CHD patients (113) and healthy controls (118) were enrolled from the First People’s Hospital of Yuhang between January 2013 and June 2014. The patients were divided into mild CHD (N = 72) and severe CHD (N =.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016954
- DOI:
- 10.4238/gmr.15016954
Use of simple sequence repeat markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp) cultivars resistant and susceptible to red rot
Authors: U. Hameed, Y.-B. Pan, K. Muhammad, S. Afghan and J. Iqbal
Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers w.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.8.1
- DOI:
- 10.4238/2012.May.8.1