Polymerase Chain Reaction
Differential expression of hENT1 and hENT2 in colon cancer cell lines
Authors: Y. Liu, T. Zuo, X. Zhu, N. Ahuja and T. Fu
Human equilibrative nucleoside transporters (hENT) 1 and 2, encoded by SLC29A1 and SLC29A2, permit the bidirectional passage of nucleoside analogues into cells and may correlate with clinical responses to chemotherapy in patients with colorectal cancer (CRC). The purpose of this study was to evaluate the expression profiles of.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019549
- DOI:
- 10.4238/gmr16019549
Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR
Authors: A.G. Dibbern, B.G. Botaro, M.P. Viziack, L.F.P. Silva and M.V. Santos
The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.6
- DOI:
- 10.4238/2015.January.16.6
Genetic diversity of Saccharum officinarum accessions in Pakistan as revealed by random amplified polymorphic DNA
Authors: F.A. Khan, A. Khan, F.M. Azhar and S. Rauf
Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clon.. Read More»
- Genet. Mol. Res. 8(4):
Expression of the ROR�± gene in Inner Mongolian cashmere goat hair follicles
Authors: Y.H. Zhao, Z.H. Liu, L. Wang, H.M. Xiao, C.G. Du, Y.J. Zhang,R. Su and J.Q. Li
The expression of retinoid-acid-related orphan receptor Ã?± (RORÃ?±) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that RORÃ?±was expressed.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.11
- DOI:
- 10.4238/2015.January.23.11
Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples
Authors: M.I. Espinosa, A. Bertin, F.A. Squeo, A. Cort�©s and N. Gouin
Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control regi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.13
- DOI:
- 10.4238/2015.January.23.13
Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology
Authors: E. Costa-Lira, A.H.V.L. Jacinto, L.M. Silva, P.F.R. Napole�£o,R.A.A. Barbosa-Filho, G.J.S. Cruz, S. Astolfi-Filho and C.M. Borborema-Santos
Sexually transmitted infections are an important cause of morbidity among sexually active women worldwide, and have been implicated as cofactors in the pathogenesis of cervical cancer. We investigated the prevalence of human papillomavirus (HPV), Chlamydia trachomatis (CT), and Trichomonas vaginalis (TV), and accessed the dive.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029626
- DOI:
- 10.4238/gmr16029626
A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea)
Authors: Euglena gracilis; Sodium dodecyl sulfate-Tris;RNA isolation; Reverse transcription-polymerase chain reaction
RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit .. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr590
- DOI:
- 10.4238/vol8-2gmr590
Prevalence of �²-lactamase classes A, C, and D among clinical isolates of Pseudomonas aeruginosa from a tertiary-level hospital in Bangkok, Thailand
Authors: C. Katvoravutthichai, K. Boonbumrung and R. Tiyawisutsri
Pseudomonas aeruginosa is one of the most important causes of nosocomial infection and it has increasing resistance to many antimicrobial agents. β-lactamase production is the most frequent mechanism for β-lactam resistance in P. aeruginosa. We evaluated the prevalence of β-lactamase genes in P. aeruginosa for classes A, C, and D by polymer.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038706
- DOI:
- 10.4238/gmr.15038706
Biological characteristics and mating type distribution of Phytophthora capsici from China
Authors: Y. Du, Z.-H. Gong, G.-Z. Liu, G.-X. Chai and C. Li
Phytophthora capsici from seven provinces of China were investigated for their mating type, hyphal growth, zoospore production, and virulence. All of the morphological characteristics and the results of polymerase chain reaction confirmed that these isolates were indeed Phytophthora capsici. The test of mating type showed that.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.21.7
- DOI:
- 10.4238/2014.January.21.7
LAMP-PCR detection of ochratoxigenic Aspergillus species collected from peanut kernel
Authors: H.M. Al-Sheikh
Over the last decade, ochratoxin A (OTA) has been widely described and is ubiquitous in several agricultural products. Ochratoxins represent the second-most important mycotoxin group after aflatoxins. A total of 34 samples were surveyed from 3 locations, including Mecca, Madina, and Riyadh, Saudi Arabia, during 2012. Fungal contamination frequency was det.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.5
- DOI:
- 10.4238/2015.January.30.5