Polymerase Chain Reaction
Identification of hub genes and pathways associated with retinoblastoma based on co-expression network analysis
Authors: Q.L. Wang, X. Chen, M.H. Zhang, Q.H. Shen, Z.M. Qin
The objective of this paper was to identify hub genes and pathways associated with retinoblastoma using centrality analysis of the co-expression network and pathway-enrichment analysis. The co-expression network of retinoblastoma was constructed by weighted gene co-expression network analysis (WGCNA) based on differentially ex.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.8.4
- DOI:
- 10.4238/2015.December.8.4
Comparison of extraction methods of total microbial DNA from freshwater
Authors: P. Li, S.F. Yang, B.B. Lv, K. Zhao, M.F. Lin, S. Zhou, X. Song and X.M. Tang
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugatio.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.16
- DOI:
- 10.4238/2015.January.30.16
Distribution of Candida albicans in the oral cavity of children aged 3-5 years of Uygur and Han nationality and their genotype in caries-active groups
Authors: N. Wu, J. Lin, L. Wu and J. Zhao
We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur c.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.18
- DOI:
- 10.4238/2015.January.30.18
Chromosome instability in industrial strains of Saccharomyces cerevisiae batch cultivated under laboratory conditions
Authors: Lucena BTL, Silva-Filho EA, Coimbra MRM, Morais JOF, Simoes DA and Morais Jr. MA
Industrial ethanol fermentation is a complex microbiological process to which yeast cells must adapt for survival. One of the mechanisms for adaptation is thought to involve chromosome rearrangements. We found that changes in chromosome banding patterns measured by pulsed-field gel electrophoresis can also be produced in labor.. Read More»
- Genet. Mol. Res. 6(4):
Identification of PML/RAR�± fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization
Authors: A. Choughule S. Polampalli P. Amre S. Shinde S. Banavali K. Prabhash R. Nair P.G. Subramanian S. Gujral P.M. Parikh
Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr488
- DOI:
- 10.4238/vol8-1gmr488
An improved extraction protocol for metagenomic DNA from a soil of the Brazilian Atlantic Rainforest
Authors: J.H. Amorim, T.N.S. Macena, G.V. Lacerda-Junior, R.P. Rezende, J.C.T. Dias, M. Brendel and J.C.M. Cascardo
The need for the prospecting for and identification of new biomolecules is a reality. Molecular techniques allow access to the metabolic potential of microorganisms via the isolation of DNA from environmental samples, i.e., without the application of microbial culture techniques. With its great biological diversity, the Atlantic Rainforest biome has a soi.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr509
- DOI:
- 10.4238/vol7-4gmr509
Mutations in WT1 in boys with sporadic isolated steroid-resistant nephrotic syndrome
Authors: Y. Yang1,2,3*, F. Zhao1,2,3*, X. Tu4 and Z. Yu1,2,3
Mutations in the Wilms’ tumor gene, WT1, can lead to syndromic steroid-resistant nephrotic syndrome and isolated steroid-resistant nephrotic syndrome. WT1 mutations have been identified in the majority of children with Denys-Drash or Frasier syndrome. WT1 mutations have not previously been identified in boys with sporadic isolated steroid-resistant .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017559
- DOI:
- 10.4238/gmr.15017559
Obese gene polymorphism in Pietrain and large white pigs after a divergent selection
Authors: A.C.P. Silveira, R.C. Antunes, J.F. Almeida, T.F. Braga, P.F.A. Freitas, A.S.M. C�©sar and E.C. Guimar�£es
The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fra.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr511
- DOI:
- 10.4238/vol7-4gmr511
Quantitative real-time polymerase chain reaction as an efficient molecular tool for detecting minimal residual disease in Moroccan chronic myeloid leukemia patients
Authors: A. Moumen, H. Dehbi, D. Kottwitz, M. El Amrani,N. Bouchoutrouch, H. El Hadi, A. Quessar, S. Benchekroun,S. Nadifi and H. Sefrioui
Chronic myeloid leukemia (CML) is characterized by BCR-ABL translocation and an increased number and migration of immature myeloid cells into the peripheral blood. The detection limit of the BCR-ABL transcript, particularly after treatment, is controversial. In the present study, we used quantitative real-time reverse transcription-polymerase chain reacti.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.8
- DOI:
- 10.4238/2015.February.6.8
Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR
Authors: E. Zamudio-Moreno, J.H. Ramirez-Prado, O.A. Moreno-Valenzuela and L.A. Lopez-Ochoa
Papaya meleira disease was identified in Brazil in the 1980s. The disease is caused by a double-stranded RNA virus known as Papaya meleira virus (PMeV), which has also been recently reported in Mexico. However, previously reported PMeV primers failed to diagnose the Mexican form of the disease. A genomic approach was used to identify sequences of the Mexi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.18
- DOI:
- 10.4238/2015.February.6.18