Polymerase Chain Reaction
Evaluation of genetic diversity in a natural rosewood population (Dalbergia nigra Vell. Allemao ex Benth.) using RAPD markers
Authors: Juchum FS, Leal JB, Santos LM, Almeida MP, Ahnert D and Correa RX
Dalbergia nigra (rosewood) is a long-lived leguminous species, which is endemic to the Brazilian Atlantic forest. Because of the high economic value of its wood, this species has been over-explored in recent years. Currently, rosewood is included in the IUCN Red List as vulnerable. We examined the genetic diversity of 87 speci.. Read More»
- Genet. Mol. Res. 6(3):
Characterization of mitochondrial genotypes in the foundation herd of the Canchim beef cattle breed
Authors: S.C. M?©o C.R. Ferreira M.R. Chiaratti F.V. Meirelles L.C.A. Regitano M.M. Alencar P.F. Barbosa
The Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis... Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr577
- DOI:
- 10.4238/vol8-1gmr577
Comparison of methods to isolate DNA from Caesalpinia ferrea
Authors: C.C. Sousa, S.O. Gomes, A.C.A. Lopes, R.L.F. Gomes, F.B. Britto, P.S.C. Lima and S.E.S. Valente
Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpini.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.16.7
- DOI:
- http://dx.doi.org/10.4238/2014.June.16.7
Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction
Authors: F.A. Martins P.C.S. Carneiro C.T. Guimar?£es J.V. Magalh?£es J.E.S. Carneiro C.D. Cruz
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, consideri.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr585
- DOI:
- 10.4238/vol8-1gmr585
Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots
Authors: C.R. de Souza F.J. Arag?£o E.C.O. Moreira C.N.M. Costa S.B. Nascimento L.J. Carvalho
Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be neces.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr560
- DOI:
- 10.4238/vol8-1gmr560
Molecular and chromosomal mutations among children with B-lineage lymphoblastic leukemia in Brazilâ??s Federal District
Authors: D.R. Mesquita J.C. C?³rdoba ?.Q. Magalh?£es M.S. C?³rdoba J.R.C. Oliveira A. Gon?§alves I. Ferrari C. Martins-de-S?¡
Acute lymphoblastic leukemia (ALL) accounts for approximately 80% of all acute leukemias during childhood. Chromosomal anomalies resulting from gene fusion, which are frequent in leukemias, create hybrid transcripts, the great majority of which encode transcription factors. We analyzed 88 pediatric patients (median age 7.3 yea.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr582
- DOI:
- 10.4238/vol8-1gmr582
Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense
Authors: S.F. Jiang, Y.P. Zhang, S.M. Sun, Y.S. Gong, Y.W. Xiong, H. Qiao, W.Y. Zhang, S.B. Jin and H.T. Fu
Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends t.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.4
- DOI:
- 10.4238/2015.May.18.4
Determining the role of a probiotic in the restoration of intestinal microbial balance by molecular and cultural techniques
Authors: Affhan Shoaib, W. Dachang and Y. Xin
The human intestine has a vast variety of microorganisms, and their balance is dependent on several factors. Antibiotics affect microfloral balance and allow naturally opportunistic organisms to multiply. Azithromycin is the most widely used macrolide antibiotic, active against a wide number of pathogens including Pseudomonas aeruginosa and Staphylococcus.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.8
- DOI:
- 10.4238/2015.February.20.8
Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica
Authors: H.Y. Yang, X.W. Xia, W. Fang, Y. Fu, M.M. An and M.B. Zhou
Spontaneous leaf color variation in bamboo provides the opportunity to study the mechanisms of leaf color formation and the breeding of ornamental bamboos. Despite the fact that many genes are known to be involved in leaf color variation in model plants, molecular mechanisms governing natural leaf color variation in bamboo hav.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.2.16
- DOI:
- 10.4238/2015.October.2.16
Transcriptional analysis of the porcine TTID gene and association of different TTID genotypes with carcass traits
Authors: J. Wang, Y.-P. Feng, B. Zuo, Y.-Z. Xiong and C.-Y. Deng
The titin immunoglobulin domain (TTID) protein localizes to the Z line in muscle and binds to alpha-actinin and gamma-filamin. It plays an indispensable role in stabilizing and anchoring of thin filaments. In this study, the 5'-regulatory region of the porcine TTID gene was analyzed with bioinformatic methods. Another objectiv.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.27.4
- DOI:
- http://dx.doi.org/10.4238/2014.February.27.4