Polymerase Chain Reaction
Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis
Authors: F. Ahmad, A.I. Khan, F.S. Awan, B. Sadia, H.A. Sadaqat and S. Bahadur
Genetic diversity analysis of chickpea germplasm can provide practical information for the selection of parental material and thus assist in planning breeding strategies. Chickpea seed is a good source of carbohydrates and proteins, constituting 80% of the total dry seed weight. Released cultivars and advanced lines of 30 chic.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr862
- DOI:
- 10.4238/vol9-3gmr862
Temporal and tissue expression of genes involved in buds of earliness cotton cultivar
Authors: V.G.L. Batista, M.P.N. Pinheiro, P.A. Melo Filho, R.C. Santos and L.M. Lima
Gene sequences previously identified in Arabidopsis buds were used as references in order to estimate temporal and tissue expression in buds, leaves, stem, and root tissues in cotton plants. Buds were evaluated during 3 phases: 2-8, 10-12, and 14-20 mm. Primers were designed for the ARF6, ATFY, and SEUSS genes for use in semi-quantitative reverse transcri.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.14
- DOI:
- 10.4238/2015.July.3.14
Optimization of chloroplast microsatellite PCR conditions and primer screening for endangered Rheum officinale, Rheum palmatum, and Rheum tanguticum
Authors: Y. Zhou, Z.J. Guo, L. Han, Y. Li and X.M. Wang
Chloroplast microsatellite primers were developed in order to provide more population genetic information of endangered Rheum officinale, R. palmatum, and R. tanguticum for conservation. The dried roots and rhizomes of these plants are important in traditional Chinese medicine. The results showed that the optimum concentrations .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.29.6
- DOI:
- 10.4238/2014.July.29.6
Evaluation of five protocols for DNA extraction from leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris
Authors: K. Aubakirova1,2, M. Omasheva1, N. Ryabushkina1, T. Tazhibaev2,G. Kampitova2 and N. Galiakparov1
Leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality DNA extraction performance. In this study, five extraction protocols were compared for their ability to produce good quality DNA from fresh and dried (with silica gel) leaves of t.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.27.13
- DOI:
- http://dx.doi.org/10.4238/2014.February.27.13
Genetic analysis of STR markers on chromosome 21 in a Han population from southeast China
Authors: Y.N. Zhu, S.M. Lu, M. Wang, F.X. Shen, Y. Chen and J.J. Hu
Short tandem repeats (STRs) are highly polymorphic sequences and have been extensively used as genetic markers in mapping studies, disease diagnosis, and human identity testing. In this study, 11 STR markers on chromosome 21, including D21S1432, D21S11, D21S1246, D21S1412, D21S1437, D21S1442, D21S2039, D21S1270, D21S1435, D21S1409, and D21S1446, were anal.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.18
- DOI:
- 10.4238/2015.March.6.18
Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design
Authors: B. Zeng, X. Huang, L.K. Huang, J. Zhang, H.D. Yan, D. Luo, H. Liang and Y. Yuan
The effects of 5 factors (template DNA, Mg2+, dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymorphism (SCoT)-PCR system of Dactylis glomerata L., using an orthogonal design L16 (45). A suitable SCoT-PCR system for D. glomerata was established; the 20 μL reaction v.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.15
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.15
Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes
Authors: F.V.M. Rubatino, N.V. Carobin, M.L. Freitas, V.T. de Oliveira, R.X. Pietra, P.P.R. Oliveira, A.A. Bosco and F.S. Jehee
High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, prime.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.12
- DOI:
- 10.4238/2015.July.14.12
Cut-and-paste-based cloning strategy for large gene site-directed mutagenesis
Authors: C. Wang, T.Y. Wang, L.Y. Zhang, X.J. Gao, X.W. Wang and C.J. Jin
Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be perf.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.10
- DOI:
- 10.4238/2015.May.25.10
Copy number and integration sites in growth hormone transgenic goats
Authors: Q. Zhang, J. Lin, Q.H. Yu, W.W. Hu and Q. Yang
Transgenic goats have been utilized for years to produce valuable protein. However, when transgenic goats are produced by random integration of inserted genes into cells, the copy number and integration sites of these genes in the goat genome are typically indefinite. Most polymerase chain reaction (PCR)-based methods that have been utilized to determine .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.10
- DOI:
- 10.4238/2015.March.20.10
14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy
Authors: D.M. Xue, X.Q. Guo, R. Chen, Z.P. Niu and C.S. Xu
14-3-3 Proteins are a ubiquitous family of molecules that participate in protein kinase signaling pathways in all eukaryotic cells. Functioning as phosphoserine/phosphothreonine-binding modules, 14-3-3 proteins participate in the phosphorylation-dependent protein-protein interactions that control progression through the cell cycle, initiation and.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.12
- DOI:
- 10.4238/2015.March.20.12