Polymerase Chain Reaction
Effect of skeletal muscle fibers on porcine meat quality at different stages of growth
Authors: F. Wu, J.J. Zuo, Q.P. Yu, S.G. Zou, H.Z. Tan, J. Xiao, Y.H. Liu and D.Y. Feng
In this study, we examined changes in meat quality and content of muscle types during porcine growth. The influence of the longissimus dorsi muscle fiber composition on meat quality and the correlation between 2 fiber-typing methods (histochemistry and real-time quantitative polymerase chain reaction) were examined. Type IIx and type IIb fibers accounted .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.13
- DOI:
- 10.4238/2015.July.14.13
Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape
Authors: J.J. Fan, P. Wang, X. Xu, K. Liu, Y.Y. Ruan, Y.S. Zhu1, Z.H. Cui and L.J. Zhang
Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage through the repeated application of fungicides. The nucleotide-binding site leucine-rich repeat gene family functions in plant immunoactivity against various pathogens and pests. In this study, the 5'.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.4
- DOI:
- 10.4238/2015.July.17.4
Screening of differentially-expressed genes in the muscles of rabbit breeds with expression profile chip
Authors: W.Z. Wang, T. Li, L.J. Shi, X.R. Yan, Y.L. Pan and X.S. Wu
The molecular mechanism underlying muscle development in rabbits is not well-understood. In the current study, differentially-expressed genes were scanned using an expression profile chip in New Zealand white rabbits (introduced breed) and Fujian yellow rabbits (local breed), and some of the genes were tested using reverse transcription-polymerase chain r.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.12
- DOI:
- 10.4238/2015.July.17.12
Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers
Authors: Alexandre Missiaggia and Dario Grattapaglia
We extended the concept of fluorescent microsatellite genotyping with a single-universal tailed primer to the simultaneous use of three different tailed primers to allow multiplexed 4-color detection for medium throughput genotyping of plant species. The method was tested on Eucalyptus DNA samples using three forward primer se.. Read More»
- Genet. Mol. Res. 5(1):
Expression of IL-1, IL-6, TNF-�±, and iNOS in pregnant women with periodontal disease
Authors: C.C.M. Otenio I. Fonseca M.F. Martins L.C. Ribeiro N.M.S.P. Assis A.P. Ferreira R.A. Ribeiro
Periodontal disease is one of the most prevalent oral diseases. An association between this disease and pregnancy has been suggested, but available findings are controversial. We evaluated the expression levels of interleukins (IL-1β and IL-6), tumor necrosis factor-alpha (TNF-α), and inducible nitric oxide synthase (iNOS) in pregnant women wit.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.20.3
- DOI:
- 10.4238/2012.September.20.3
Epstein-Barr virus DNA associated with gastric adenocarcinoma and adjacent non-cancerous mucosa in patients from Manaus, Brazil
Authors: P.F. de Aquino P.C. Carvalho J.S. da Gama Fischer A.Q.L. de Souza J.S. Viana S.R.S. Chalub A.D.L. de Souza M.G.C. Carvalho
Gastric cancer is one of most frequent causes of death in Brazil. The city of Manaus has one of the highest incidences of this disease in Brazil. The Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that is classified as a group 1 carcinogen by the International Agency for Research on Cancer. We obtained biopsies from 6 control subjects and 10 patient.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.15.3
- DOI:
- 10.4238/2012.October.15.3
Simplified preparation of a DNA ladder using PCR
Authors: T.-Y. Wang, L. Wang and F. Wang
Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1177
- DOI:
- 10.4238/vol10-3gmr1177
Identification of key biomarkers involved in osteosarcoma using altered modules
Authors: Z.Z. Liu, S.T. Cui, B. Tang, Z.Z. Wang and Z.X. Luan
The aim of this study was to screen for key biomarkers of osteosarcoma (OS) by tracking altered modules. Protein-protein interaction (PPI) networks of OS and normal groups were constructed and re-weighted using the Pearson correlation coefficient (PCC), respectively. The condition-specific modules were explored from OS and normal PPI networks using a cliq.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038277
- DOI:
- 10.4238/gmr.15038277
Differential expression of E-cadherin gene in human neuroepithelial tumors
Authors: F.J.N. Motta, E.T. Valera, A.K.B. Lucio-Eterovic, R.G.P. Queiroz,L. Neder, C.A. Scrideli, H.R. Machado, C.G. Carlotti-Junior, S.K.N. Marie and L.G. Tone
Cadherins are cell-to-cell adhesion molecules that play an important role in the establishment of adherent-type junctions by mediating calcium-dependent cellular interactions. The CDH1 gene encodes the transmembrane glycoprotein E-cadherin which is important in maintaining homophilic cell-cell adhesion in epithelial tissues. E.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr424
- DOI:
- 10.4238/vol7-2gmr424
No evidence for association between DRD3 and COMT with schizophrenia in a Malay population
Authors: S.F. Tee, P.Y. Tang and H.C. Loh
Molecular components of the dopamine D3 receptor (DRD3) may play an important role in the pathophysiology of schizophrenia. Previous studies have demonstrated an association between DRD3 Ser9Gly and cathechol-o-methyltransferase (COMT, SNP = rs165656) polymorphisms and schizophrenia but the results were inconclusive. We invest.. Read More»
- Genet. Mol. Res. 10(3):