Polymerase Chain Reaction
Molecular characteristics and expression of calmodulin cDNA from the freshwater pearl mussel, Hyriopsis schlegelii.
Authors: L.-G. Zeng, J.-H. Wang, Y.-J. Li, J.-Q. Sheng, Q. Gu and Y.-J. Hong
Calmodulin (CaM) is a multifunctional intracellular calcium ion receptor protein that participates in a range of cellular processes, including calcium metabolism in mussels. To investigate the role of CaM in freshwater mollusk shell calcium metabolism, the full-length CaM cDNA was isolated from the freshwater pearl mussel, Hyr.. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.9.5
- DOI:
- http://dx.doi.org/10.4238/2012.January.9.5
KIR gene polymorphism study in the Uygur population in Xinjiang, China
Authors: G.-Y. Lin and Y.-B. Wang
We analyzed killer cell immunoglobulin-like receptor (KIR) gene polymorphisms and genotype and haplotype characteristics in the Uygur population, a non-nomadic ethnic group found in Xinjiang, China, to provide a basis for studies on relationships between KIRs and diseases in this group. Sequence-specific primer PCR was used to detect the KIR gene in 84 Uy.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.9
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.9
Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites
Authors: I.G. Hamoy, E.J.M. Santos and S.E.B. Santos
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtain.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr394
- DOI:
- 10.4238/vol7-1gmr394
A/G Gln20Arg (exon 1) and G/A Val156Met (exon 5) polymorphisms of the human orosomucoid 1 gene in Mexico
Authors: L. GarcÃ?Âa-Ortiz, G. Vargas-AlarcÃ?³n, J.M. Fragoso, J. Granados, L. Maldonado Noriega, A. Navas PÃ?©rez , E. Huerta Reyes, J.C. Zenteno-Ruiz and E. MartÃ?Ânez-Cordero
The human orosomucoid 1 gene (ORM1) codes an alpha-1-acid glycoprotein that has been classified as an acute-phase reactive protein, and a major drug-binding serum component, as well as an immunomodulatory protein with genetic polymorphisms. Evaluation of ORM variation through isoelectric focusing and immunobloting has revealed a world-wide distribution of.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr348
- DOI:
- 10.4238/vol7-1gmr348
Molecular basis for porcine parvovirus detection in dead fetuses
Authors: V.H.G. Wolf , M. Menossi, G.B. Mour�£o and M.S.V. Gatti
Reproductive failures are still common grounds for complaint by commercial swine producers. Porcine parvovirus (PPV) is associated with different clinical reproductive signs. The aim of the present study was to investigate PPV fetal infection at swine farms having ongoing reproductive performance problems. The presence of viru.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr440
- DOI:
- 10.4238/vol7-2gmr440
Identification of significantly different modules between permanent and deciduous teeth by network and pathway analyses
Authors: J. Kang, R. Bai, K. Liu, X.P. Ji, Y. Li and J.Y. Han
The differences in genetic backgrounds between deciduous and permanent teeth might contribute to the differences in developmental processes, histological characteristics, and tooth life cycles. Here, we attempted to identify significantly different modules between permanent and deciduous teeth via network and pathway analyses... Read More»
- Genet. Mol. Res. 15(4):
- gmr15047959
- DOI:
- 10.4238/gmr15047959
Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA
Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.20
- DOI:
- 10.4238/2015.July.28.20
Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds
Authors: G.E. Oliveira, E.V.R. Von Pinho, T. Andrade, J.C. Souza, F. Caixeta and R.A.D.C. Ferreira
In this study, we analyzed heterosis, amylase enzyme gene expression, and the physiological quality of maize seeds with different genotypes and sizes, which were subjected to aging and not subjected to aging. We used seeds from 2 maize lines that differed with regard to physiological quality, the hybrid, and the reciprocal hybrid; they were classified int.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.10
- DOI:
- 10.4238/2015.July.31.10
Genetic characterization of hatchery populations of Korean spotted sea bass (Lateolabrax maculatus) using multiplex polymerase chain reaction assays
Authors: H.S. An, H.Y. Kim, J.B. Kim, D.S. Chang, K.D. Park, J.W. Lee, J.I. Myeong and C.M. An
The spotted sea bass, Lateolabrax maculatus, is an important commercial and recreational fishery resource in Korea. Aquacultural production of this species has increased because of recent resource declines, growing consumption, and ongoing government-operated stock release programs. Therefore, the genetic characterization of h.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.14
- DOI:
- 10.4238/2014.August.28.14
Genetic polymorphism at the KIR gene locus: determination of gene, genotype, and haplotype frequencies in the Xinjiang Han population
Authors: G.-Y. Lin, B. Yu, W.-J. Hu, Y.-Z. Zhang, X.-J. Zuo and Y.-B. Wang
The aim of this study was to explore the genetic polymorphism, genotype, and haplotype characteristics of the KIR locus in the Xinjiang Han population in order to establish a foundation for future analysis of the relationship between KIR genes and disease. KIR genes were detected by sequence-specific primer-polymerase chain reaction in 184 randomly select.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.9.7
- DOI:
- 10.4238/2015.June.9.7