Polymerase Chain Reaction
Genetic variations of 21 STR markers on chromosomes 13, 18, 21, X, and Y in the south Iranian population
Authors: J. Saberzadeh, M.R. Miri, M.B. Tabei, M. Dianatpour and M. Fardaei
Quantitative fluorescent polymerase chain reaction (QF-PCR), in recent years, has been accepted as a rapid, high throughput, and sensitive method for prenatal diagnosis of common chromosomal aneuploidies. Since short tandem repeats (STRs) are the cornerstone of QF-PCR technique, selection of the most polymorphic STR markers is.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049065
- DOI:
- 10.4238/gmr15049065
Study of the correlation between GH gene polymorphism and growth traits in sheep
Authors: J.L. Jia, L.P. Zhang, J.P. Wu, Z.J. Ha and W.W. Li
The growth hormone gene plays an important role in the physiological function of an organism. The current study aimed to investigate the correlation between polymorphisms in the 5êÂ?Â? regulatory region, exon 4, and 3êÂ?Â? untranslated region (UTR) of the sheep GH gene and sheep growth traits. The DNA from 510 adult sheep wa.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.5.5
- DOI:
- 10.4238/2014.September.5.5
Effect of MDR1 polymorphism on multidrug resistance expression in breast cancer patients
Authors: M. Taheri, F. Mahjoubi and R. Omranipour
One of the limitations in the treatment of cancer patients with chemotherapy is the development of multidrug resistance (MDR). A well-known mechanism responsible for drug resistance is over-expression of ABC-transporter genes such as MDR1. This gene encodes p-glycoprotein (P-gp), a transmembrane glycoprotein that transports ma.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr669
- DOI:
- 10.4238/vol9-1gmr669
A rapid method for isolation of total DNA from pathogenic filamentous plant fungi
Authors: D. GonzÃ?¡lez-Mendoza, R. Argumedo-Delira, A. Morales-Trejo, A. Pulido-Herrera, L. Cervantes-DÃ?Âaz, O. Grimaldo-Juarez and A. AlarcÃ?³n
DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terre.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr680
- DOI:
- 10.4238/vol9-1gmr680
Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia
Authors: C. Guti�©rrez-Vergara, J. Quintero, J.F. Duarte, J.P. Suesc�ºn and A. L�³pez-Herrera
Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.31.20
- DOI:
- 10.4238/2015.March.31.20
Association between the TRAIL single nucleotide polymorphism rs1131580 and type 2 diabetes mellitus in a Han Chinese population
Authors: M.Y. Yu, P.Q. Zhao, X.H. Yan, B. Liu, Q.Q. Zhang, R. Wang, C.H. Ma, X.H. Liang, F.L. Zhu and L.F. Gao
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is expressed in different tissues and cells, including the pancreas and lymphocytes, and it can selectively induce apoptosis in tumor cells but not in most normal cells. TRAIL plays critical roles in type 1 diabetes mellitus, and is involved in type 2 diabetes mellitus (T2DM). We recent.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.10.2
- DOI:
- 10.4238/2013.September.10.2
A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression
Authors: H. Li, Q. Jiang, Z.H. Ju, J.M. Huang, X.G. Wang, C.H. Yang, Y. Sun, G.W. Yang, J.F. Zhong and C.F. Wang
The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain react.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027723
- DOI:
- 10.4238/gmr.15027723
Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction
Authors: Z.J. Wei, F.Y. Wang, M.P. Guo, Z.Z. Duan, N.L. Zou, P. Liu, Q.G. Yan, X.T. Wen, S.J. Cao and Y. Huang
Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.9
- DOI:
- 10.4238/2015.June.11.9
A simplified genomic DNA extraction protocol for pre-germination genotyping in rice
Authors: Y.B. Duan, F.L. Zhao, H.D. Chen, H. Li, D.H. Ni, P.C. Wei, W. Sheng, J.T. Teng, A.M. Zhang and J.P. Xue
Genotyping is a critical step for molecular markerassisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, whi.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.12
- DOI:
- 10.4238/2015.June.11.12
Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil
Authors: A.C. Santini, J.T. Magalh�£es, J.C.M. Cascardoâ� and R.X. Corr�ªa
Chromobacterium violaceum is a free-living Gram-negative bacillus usually found in the water and soil in tropical regions, which causes infections in humans. Chromobacteriosis is characterized by rapid dissemination and high mortality. The aim of this study was to detect the genetic variability among C. violaceum type strain A.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027955
- DOI:
- 10.4238/gmr.15027955