Proliferation
Regulatory role of microRNA184 in osteosarcoma cells
Authors: G.R. Yin1,2, Q. Wang3, X.B. Zhang2 and S.J. Wang1
Osteosarcoma is a highly malignant cancer that often appears in teenagers. It is the most frequently occurring primary bone tumor, and can easily metastasize, resulting in high mortality. MicroRNAs express abnormally in osteosarcoma, and may function as oncogenes or tumor suppressors. Recent studies showed that microRNA184 (miR-184) is abnormally expresse.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.8
- DOI:
- 10.4238/2015.November.13.8
Effect of progranulin (PGRN) on the proliferation and senescence of cervical cancer cells
Authors: X.Y. Zhang1, Z.X. Pan1, H. Liu1, J.L. Yu1, G.X. Li2, H.Y. Wang3 and M.M. Liu4
We investigated the effect of progranulin (PGRN) expression on the proliferation and senescence of cervical cancer cells. PGRN small interfering RNA (siRNA) was introduced into the SiHa and HeLa cell lines of human cervical carcinoma using liposome-mediated transfection. The expression levels of PGRN in each cell line after transfection of PGRN siRNA were.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.18
- DOI:
- 10.4238/2015.November.13.18
Effect of Bcl-2-siRNA on proliferation and apoptosis of pediatric acute B lymphoblastic leukemia (A-BLL) cells
Authors: W.B. Meng, J.P. Liu, X.W. Wang and L.H. E
This study analyzed the effect of small interfering RNA specific for the Bcl-2 gene (siRNA Bcl-2) on the proliferation and chemotherapeutic sensitivity of pediatric A-BLL cells. Marrow samples were obtained from sixty newly-diagnosed A-BLL pediatric patients. The Bcl-2 mRNA expression in these samples was quantified by real ti.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.9
- DOI:
- 10.4238/2015.October.16.9
Inhibition of invasion and migration of prostate cancer cells by miRNA-509-5p via targeting MDM2
Authors: X.M. Tian, Y.Z. Luo, P. He, J. Li, Z.W. Ma and Y. An
Prostate cancer is a common malignancy of the male reproductive-urinary system. MDM2 is an oncogene, whose expression can be regulated by microRNA (miRNA). The present study investigated the expression and correlation of miRNA-509-5p and MDM2 to determine the mechanism of their function in invasion and migration of prostate ca.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019195
- DOI:
- 10.4238/gmr16019195
Effects of Cx43 gene modification on the proliferation and migration of the human lung squamous carcinoma cell line NCI-H226
Authors: J.-P. Zang and R. Wei
In this study, the human lung squamous carcinoma cell line NCI-H226 was transfected with the recombinant plasmid pBudCE4.1_Cx43 to explore the role of the Cx43 gene in cell growth, cell cycle, and tumor migration. pBudCE4.1-Cx43 was transfected into human lung squamous carcinoma NCI-H226 cells using Lipofectamine TM2000. The mRNA and protein expressions o.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.7
- DOI:
- 10.4238/2015.October.26.7
Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase
Authors: Y.-J. Shi K.-K. Huo
Anaphase-promoting complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which catalyses ubiquitination of cell-cycle regulators. Studying this complex could reveal important information regarding its application in cancer research and therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were transfected into HEK293T ce.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.6
- DOI:
- 10.4238/2012.August.24.6
Inhibition of miR-660-5p expression suppresses tumor development and metastasis in human breast cancer
Authors: Y. Shen, Y.F. Ye, L.W. Ruan, L. Bao, M.W. Wu and Y. Zhou
Breast cancer, which derives from the epithelium of the mammary glands, is one of the most common cancers diagnosed in women globally. To date, the authors of many studies have reported that the deregulation of microRNAs (miRNAs) plays a crucial role in the occurrence, development, and metastasis of tumors. Here, we discovered.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019479
- DOI:
- 10.4238/gmr16019479
Effects of miRNA-143 and the non-coding RNA MALAT1 on the pathogenesis and metastasis of HeLa cells
Authors: L. Zhang, H.E.X.D. Niyazi, H.R. Zhao, X.P. Cao, M.N.S. Abudula, W.J. Ye, S.A. Zhang, R.H.M. Yiming, Y. Zhang, W.P. Su, R. Chen, Y. Ouyang, N. Miao and Y.X. Bao
Cervical cancer is a common female malignancy of global dimensions. MicroRNAs (miRNAs) play crucial roles in the development, differentiation, proliferation, and apoptosis of tumors. The non-coding RNA MALAT1 participates in various physiological processes that are important for proper functioning of the body. Here, we analyze.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019269
- DOI:
- 10.4238/gmr16019269
Paracrine effect of bone marrow mesenchymal stem cells on proliferation, apoptosis, and alpha-actin-2 expression in hepatic stellate cells
Authors: H.-J. Cao, M.-D. Wang, S.-G. Li, L. Zhu, J.-H. Zheng
We investigated the paracrine effects of bone marrow mesenchymal stem cells (BMSCs) on the proliferation, apoptosis, and alpha-actin-2 (ACTA2) expression of hepatic stellate cells (HSCs), and explored the possible mechanisms of hepatocyte growth factor (HGF). We established a co-culture system by culturing BMSCs on the upper layer and HSCs on the lower la.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019201
- DOI:
- 10.4238/gmr16019201
Lenalidomide affect expression level of cereblon protein in multiple myeloma cell line RPMI8226
Authors: D.Y. Yang, J.H. Ren, X.N. Guo, X.L. Guo, X.Y. Cai, X.F. Guo and J.N. Zhang
We investigated the mechanisms of action of immunomodulatory drug (lenalidomide) on the protein expression of cereblon (CRBN) and their therapeutic targets in the multiple myeloma cell line RPMI8226. The multiple myeloma cell line RPMI8226 was cultured and treated with different concentrations of lenalidomide and bortezomib to determine the proliferation .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.19
- DOI:
- 10.4238/2015.October.28.19