Promoter
Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba
Authors: F. Xu X.H. Huang L.L. Li G. Deng H. Cheng X.F. Rong J.B. Li S.Y. Cheng
Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome walking. The .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.3
- DOI:
- 10.4238/2013.February.4.3
Characterization of a rice germin-like protein gene promoter
Authors: T. Mahmood T. Yasmin M.I. Haque S.M.S. Naqvi
Germin and germin-like proteins (GLPs) are water-soluble extracellular proteins that are expressed in response to specific environmental and developmental signals. Although some enzymatic activities have also been associated with germin/GLPs, their role in overall metabolism is not fully understood. However, insight into their function may be gained by an.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.6
- DOI:
- 10.4238/2013.February.7.6
Cloning and characterization of SPL-family genes in the peanut (Arachis hypogaea L.)
Authors: M. Li1*, S.Z. Zhao1*, C.Z. Zhao1, Y. Zhang1, H. Xia1, J. Lopez-Baltazar2, S.B. Wan1 and X.J. Wang1
SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and anal.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017344
- DOI:
- 10.4238/gmr.15017344
Highly conserved regions in the 5� region of human olfactory receptor genes
Authors: H.F. Tobar P.A. Moreno P.E. V�©lez
Regulation of human olfactory receptor (hOR) genes is a complex process of control and signalization with various structures and functions that are not clearly understood. To date, nearly 390 functional hOR genes and 462 pseudogenes have been discovered in the human genome. Enhancer models and trans-acting elements for the reg.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr550
- DOI:
- 10.4238/vol8-1gmr550
Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots
Authors: C.R. de Souza F.J. Arag�£o E.C.O. Moreira C.N.M. Costa S.B. Nascimento L.J. Carvalho
Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be neces.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr560
- DOI:
- 10.4238/vol8-1gmr560
A new inducible expression system in a transformed green alga, Chlorella vulgaris
Authors: Y.F. Niu, M.H. Zhang, W.H. Xie, J.N. Li, Y.F. Gao, W.D. Yang, J.S. Liu and H.Y. Li
Genetic transformation is useful for basic research and applied biotechnology. However, genetic transformation of microalgae is usually quite difficult due to the technical limitations of existing methods. We cloned the promoter and terminator of the nitrate reductase gene from the microalga Phaeodactylum tricornutum and used .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.21.1
- DOI:
- 10.4238/2011.October.21.1
Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)
Authors: J.D. Wu, C.P. Jiang, H.S. Zhu, H.Y. Jiang, B.J. Cheng and S.W. Zhu
The ZmDULL1 gene encodes a starch synthase and is a determinant of the structure of endosperm starch in maize (Zea mays L.). However, little is known regarding the regulatory mechanism of the ZmDULL1 gene. In this study, we isolated and characterized the ZmDULL1 promoter (PDULL1), which is the 5' flanking region of ZmDULL1 in maize. Sequence analysis show.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.17
- DOI:
- 10.4238/2015.May.22.17
rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.
Authors: X.Y. Cui, Z.Y. Chen, L. Wu, X.Q. Liu, Y.Y. Dong, F.W. Wang and H.Y. Li
The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco pl.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.15
- DOI:
- 10.4238/2015.July.3.15
Evaluation of three different promoters driving gene expression in developing chicken embryo by using in vivo electroporation
Authors: C.Q. Yang1, X.Y. Li2, Q. Li3, S.L. Fu2, H. Li2, Z.K. Guo3, J.T. Lin2,3 and S.T. Zhao1
To investigate the variance of exogenous gene expression driven by different promoters by in vivo electroporation, 3 plasmid vectors carrying different promoters were selected, and their driving strength was compared in developing chicken embryos. The 3 promoters included: 1) the CAG promoter (containing the cytomegalovirus (C.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.27.12
- DOI:
- http://dx.doi.org/10.4238/2014.February.27.12
Expression patterns and promoter activity analysis of UGP2 in pigs.
Authors: J.Y. Yu, S.M. Shao, K. Chen, M.G. Lei, and Y.Z. Xiong,
The function of the UDP-glucose pyrophosphorylase 2 gene (UGP2) in pig is not clear. In the present study, we used RNA isolated from Large White pigs and Chinese indigenous MeiShan pigs to examine the temporal coordination of changes in gene expression within muscle tissues. We cloned both the complete genomic DNA sequence and.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.28.8
- DOI:
- http://dx.doi.org/10.4238/2014.February.28.8