Authors: Improving yield and quality traits of durum wheat by introgressing chromosome segments from hexaploid wheat
Durum wheat (Triticum turgidum durum; 2n = 4x = 28; genome AABB) has long been an important food resource for human diets. The projected increase of the world’s population to 9.1 billion by 2050 has highlighted the importance and urgency for improving the yield and quality performance of durum wheat. A backcrossed popula.. Read More»
Authors: X.B. Qiao, K.Y. Xu, B. Li, X. Luan, T. Xia and X.Z. Fan
We analyzed meat samples of nine pure lines of rabbit and its 37 hybrid combinations by sequencing and single-strand conformation polymorphism techniques to explore genetic polymorphisms of all the three exon regions and part of the 5'-regulatory region of the myostatin (MSTN) gene. Thus, we detected a single nucleotide mutati.. Read More»
Authors: H. Li1,2, J. Li1,2, X.H. Cong2, Y.B. Duan2,3, L. Li2, P.C. Wei2, X.Z. Lu2 and J.B. Yang2
The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block,.. Read More»
Authors: X.H. Du, Q.F. Gan, Z.R. Yuan, X. Gao, L.P. Zhang, H.J. Gao,J.Y. Li and S.Z. Xu1
Myogenic determination factor 1 (MyoD1) and myogenic factor 6 (Myf6) genes belong to the myogenic differentiation (MyoD) gene family, which play key roles in growth and muscle development. The study aimed to investigate the effects of variants in cattle MyoD1 and Myf6 on carcass and meat traits. We screened single nucleotide polymorphisms (SNPs) of both g.. Read More»
Authors: X.D. Zhang, S.J. Zhang, Y.Y. Ding, Y.F. Feng, H.Y. Zhu, L. Huang, T. Wu, J. Zhou and Z.J. Yin
In this study, meat quality traits were compared between Chinese lard- and European lean-type pigs. The association between expression of four genes (ADSL, GARS-AIRS-GART, DGAT1, and DECR1) and meat quality traits was also investigated. Meat quality traits were found to differ significantly between pig breeds.. Read More»
Authors: X. Liu, X.Y. Guo, X.Z. Xu, M. Wu, X. Zhang, Q. Li, P.P. Ma, Y. Zhang, C.Y. Wang, F.J. Geng, C.H. Qin, L. Liu, W.H. Shi, Y.C. Wang and Y. Yu
DNA methylation is essential for adipose deposition in mammals. We screened SNPs of the bovine DNA methyltransferase 3b (DNMT3b) gene in Snow Dragon beef, a commercial beef cattle population in China. Nine SNPs were found in the population and three of six novel SNPs were chosen for genotyping and analyzing a possible association with 16 meat quality trai.. Read More»
Authors: A.A. Droval, E. Binneck, S.R.R. Marin, F.G. Pai�£o1, A. Oba, A.L. Nepomuceno and M. Shimokomaki
Some genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a .. Read More»
Authors: M. Meng, X. Gao, L.J. Han, X.Y. Li, D. Wu, H.Z. Li and Q.J. Chen
To understand the relationships between single nucleotide polymorphisms (SNPs) in the waxy gene and starch parameters in common rye, we performed sequence characterization, enzyme activity testing, amylopectin/amylose ratio evaluation, starch property testing, and correlation analysis. Specific primers were used to clone waxy .. Read More»
Authors: L.Y. Shen1, J. Luo1, H.G. Lei1, Y.Z. Jiang2, L. Bai1, M.Z. Li1, G.Q. Tang1, X.W. Li1, S.H. Zhang1 and L. Zhu1
The myosin heavy chain (MyHC) composition, glycolytic potential, mitochondrial content, and gene expression related to energy metabolism were analyzed in eight muscles from Tibetan pigs, to study how meat quality develops in different muscle tissues. The muscles were classified into three clusters, based on MyHC composition: masseter, trapezius, and latis.. Read More»
Authors: P. Klomtong, K. Chaweewan, Y. Phasuk and M. Duangjinda
Mutations in melanocortin 1 receptor (MC1R) gene and v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) gene have been shown to affect coat color patterns in pigs. Additional functional marker genes, such as insulin like growth factor-2 (IGF2) and orphan nuclear receptor, germ cell nuclear factor (NR6A1), have.. Read More»