Rapd
DNA Extraction from silica gel preserved common bean (Phaseolus vulgaris L.) leaves
Authors: Male. A.S, Kato. F, Mukankusi. C.M
Extraction of high-quality DNA from field specimen requires collection under liquid nitrogen which is not readily available in resource constrained laboratories in low- and middle-income countries (LMICs). A method of extracting DNA from silica gel preserved common bean (Phaseolus vulgaris L.) leaves is presented. Our method which does not involve the use.. Read More»
- Genet. Mol. Res. 17(4):
- http://dx.doi.org/gmr16039926
- DOI:
- http://dx.doi.org/10.4238/gmr16039926
Variability among Capsicum baccatum accessions from Goi�¡s, Brazil, assessed by morphological traits and molecular markers
Authors: A.L.A. Martinez, J.S.P. Ara�ºjo, C.F. Ragassi, G.S.C. Buso and F.J.B. Reifschneider
Capsicum peppers are native to the Americas, with Brazil being a significant diversity center. Capsicum baccatum accessions at Instituto Federal (IF) Goiano represent a portion of the species genetic resources from central Brazil. We aimed to characterize a C. baccatum working collection comprising 27 accessions and 3 commerci.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039074
- DOI:
- 10.4238/gmr16039074
Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis
Authors: A.R.E.O. Xavier, A.C. Almeida, C.N. Souza, L.M.V Silva, A.X.A. Ruas, D.A. Sanglard, A.F.M. J�ºnior , A.M.E. Oliveira and M.A.S. Xavier
The Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of ant.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029709
- DOI:
- 10.4238/gmr16029709
Evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic DNA markers associated with the resistant trait in Chinese wild Vitis
Authors: J. Zhang, Y. Zhang, H. Yu1, and Y. Wang
The resistance of wild Vitis germplasm, including Chinese and American wild Vitis and Vitis vinifera cultivars, to powdery mildew (Uncinula necator Burr.) was evaluated for two consecutive years under natural conditions. Most of the Chinese and North American species displayed a resistant phenotype, whereas all of the European.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.3
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.3
A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat
Authors: W.L. Du, J. Wang, L.M. Wang, Y.H. Pang, J. Wu, J.X. Zhao, Q.H. Yang and X.H. Chen
In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search t.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.16
- DOI:
- 10.4238/2013.October.18.16
Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer
Authors: D.Y. Lin, T.Y. Chiang, C.C. Huang, H.D. Lin, S.J. Tzeng, S.R. Kang, H.M. Sung and M.C. Wu
Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.7
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.7
Genetic diversity of spineless Cereus jamacaru accessions using morphological and molecular markers
Authors: F.I.C. Oliveira, P.N. Bordallo, A.C.R. Castro and D. Correia
This is the first study to examine the genetic diversity of mandacaru cactus (Cereus jamacaru P. DC.). Plants of spineless mandacaru are commonly found in gardens and parks of urban areas in northeastern Brazil. In addition to exploring their ornamental potential, morphological, and genetic characterization may contribute to the development of plant mater.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.17.1
- DOI:
- 10.4238/2013.October.17.1
Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers
Authors: I.G. Almeida, P. Ianella, M.T. Faria, S.R. Paiva and A.R. Caetano
Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.17
- DOI:
- 10.4238/2013.December.4.17
Genetic diversity of Cosmos species revealed by RAPD and ISSR markers
Authors: A. RodrÃ?Âguez-Bernal, J.L. PiÃ?±a-Escutia, L.M. VÃ?¡zquez-GarcÃ?Âa and A.M. Arzate-FernÃ?¡ndez
The genus Cosmos is native of America and is constituted by 34 species; 28 of them are endemic of Mexico. The cosmos are used as a nematicide, antimalarial, and antioxidative agent. The aim of this study was to estimate the genetic diversity among 7 cosmos species based on random amplified polymorphic DNA (RAPD) and inter-simple sequences repeats (ISSR) m.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.13
- DOI:
- 10.4238/2013.December.4.13
Identification and authentication of Rosa species through development of species-specific SCAR marker(s)
Authors: K.M.I. Bashir, F.S. Awan, I.A. Khan, A.I. Khan and M. Usman
Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the i.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.8