Rapd
A simple boiling-based DNA extraction for RAPD profiling of landfarm soil to provide representative metagenomic content
Authors: J.H. Amorim, R.O. Vidal, G.V. Lacerda-Junior, J.C.T. Dias, M. Brendel, R.P. Rezende and J.C.M. Cascardo
Landfarm soils are employed in industrial and petrochemical residue bioremediation. This process induces selective pressure directed towards microorganisms capable of degrading toxic compounds. Detailed description of taxa in these environments is difficult due to a lack of knowledge of culture conditions required for unknown .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.27.5
- DOI:
- http://dx.doi.org/10.4238/2012.January.27.5
Leaf morphological and genetic divergence in populations of Drimys (Winteraceae) in Chile
Authors: P. Jara-Arancio, M.R. Carmona, C. Correa, F.A. Squeo and G. Arancio
The genus Drimys is distributed in Chile from semi-arid zones to sub-Antarctic forests; there are three species of this tree, D. andina, D. confertifolia and D. winteri, the latter with varieties chilensis and winteri. Northern populations are found in small disjunct natural refuges, specifically mountain cloud forests and the.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.3.3
- DOI:
- 10.4238/2012.February.3.3
Development of a species-specific sequence-characterized amplified region marker for roses
Authors: S. Riaz, B. Sadia, F.S. Awan, I.A. Khan, H.A. Sadaqat and I.A. Khan
DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.24.3
- DOI:
- 10.4238/2012.February.24.3
RAPD-based genetic diversities and correlation with morphological traits in Camellia (Theaceae) cultivars in China
Authors: X.F. Wang, H.Y. Zheng, W.H. Zheng, C.Q. Ao, H.Y. Jin, L.H. Zhao, N. Li and L.R. Jia
Camellia is an economically important ornamental plant that has many uses, such as in beverages, foods and medicines. We examined 15 Camellia cultivars in Wenzhou, China, using RAPD markers and measurements of three traits (petal color, flower diameter, blooming period). PCR amplification with 15 random primers produced 1935 b.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1207
- DOI:
- 10.4238/vol10-2gmr1207
Genetic diversity and mycelial compatibility groups of the plant-pathogenic fungus Sclerotinia sclerotiorum in Brazil
Authors: C.G. Litholdo J�ºnior, E.V. Gomes, M. Lobo J�ºnior, L.C.B. Nasser and S. Petrofeza
The genetic variability of 40 Sclerotinia sclerotiorum isolates from various fields widely distributed throughout Brazil and different host crops was analyzed using RAPD markers and mycelial compatibility groupings (MCGs). The isolates were characterized using 16 random primers of the OPERON series, which produced 121 DNA frag.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr937
- DOI:
- 10.4238/vol10-2gmr937
Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints
Authors: J. Lin, X.C. Wang, Y.H. Chang and J.G. Fang
Accurate and reliable cultivar identification of crop species is essential to guarantee plant material identity for purposes of registration, cultivar protection and production. To facilitate identification of plant cultivars, we developed a novel strategy for efficient recording of DNA molecular fingerprints in genotyped plan.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1097
- DOI:
- 10.4238/vol10-2gmr1097
Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran
Authors: Z. Noormohammadi, H. Samadi-Molayousefi and M. Sheidai
Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 .. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.19.4
- DOI:
- 10.4238/2012.March.19.4
Molecular characterization and RAPD analysis of Juniperus species from Iran
Authors: J. Kasaian, J. Behravan, M. Hassany, S.A. Emami, F. Shahriari and M.H. Khayyat
The genus Juniperus L. (Cupressaceae), an aromatic evergreen plant, consists of up to 68 species around the world. We classified five species of Juniperus found in Iran using molecular markers to provide a means for molecular identification of Iranian species. Plants were collected (three samples of each species) from two diff.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1021
- DOI:
- 10.4238/vol10-2gmr1021
Genetic analysis of Apuleia leiocarpa as revealed by random amplified polymorphic DNA markers: prospects for population genetic studies
Authors: K.H. Lencina, E.R. Konzen, S.M. Tsai and D.A. Bisognin
Apuleia leiocarpa (Vogel) J.F. MacBride is a hardwood species native to South America, which is at serious risk of extinction. Therefore, it is of prime importance to examine the genetic diversity of this species, information required for developing conservation, sustainable management, and breeding strategies. Although scarce.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049058
- DOI:
- 10.4238/gmr15049058
Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans
Authors: S.C. Chang, D.P.C. Mac�ªdo, C.M. Souza-Motta and N.T. Oliveira
Fusarium verticillioides is a pathogen of agriculturally important crops, especially maize. It is considered one of the most important pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and animals. Moreover, it is recognized as a cause of localized infections in immunocompete.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.2
- DOI:
- 10.4238/2013.August.12.2